Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
GRP 75 Antibody (D-9) - Western Blotting - Image 356183
GRP 75 抗体 (D-9): sc-133137. K-562 (A), Jurkat (B), NIH/3T3 (C), Neuro-2A (D), KNRK (E) 和 RIN-m5F (F) 全细胞裂解液中 GRP 75 表达的 Western 印迹分析.
GRP 75 抗体 (D-9): sc-133137. K-562 (A), Jurkat (B), NIH/3T3 (C), Neuro-2A (D), KNRK (E) 和 RIN-m5F (F) 全细胞裂解液中 GRP 75 表达的 Western 印迹分析.

GRP 75 抗体 (D-9): sc-133137

4.6(25)
寫評論提問

说明书
  • GRP 75 抗体 D-9 是小鼠单克隆 IgG1 κ，GRP 75抗体, 在74篇文献中引用，规格为200 µg/ml
  • 免疫human物种的GRP 75的氨基酸525-679
  • GRP 75 抗体 (D-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 GRP 75
  • 抗GRP 75抗体（D-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取GRP 75 (D-9): sc-133137的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 GRP 75 抗体 (D-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与GRP 75 抗体 (D-9) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外Glucose-Regulated Protein抗体，包括GRP 75, GRP 78和GRP 94

GRP 75抗体（D-9）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的GRP 75蛋白。GRP 75 (D-9) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。GRP 75，也称为热休克蛋白家族A（Hsp70）成员9，在线粒体基质中起着至关重要的作用，GRP 75协助来自核DNA和线粒体DNA的新生多肽链的转运和正确折叠。这一功能对于维持线粒体的完整性和功能至关重要，因为错误折叠的蛋白质会导致线粒体功能障碍，并引发包括神经退行性疾病和癌症在内的多种疾病。在细胞压力条件下，如葡萄糖缺乏，GRP 75的表达尤为重要，它有助于确保线粒体必需蛋白的正确折叠和功能，从而支持细胞的存活和代谢平衡。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GRP 75 抗体 (D-9) 参考文献:

  1. 伴侣素 Hsp60 防止蛋白质在热应力下变性  |  Martin, J., et al. 1992. Science. 258: 995-8. PMID: 1359644
  2. 低温对小鼠 FM3A 细胞中 hsp70 mRNA 诱导及 hsp70 和 hsp105 积累的影响  |  Hatayama, T., et al. 1992. J Biochem. 111: 484-90. PMID: 1618738
  3. IP3R1/GRP75/VDAC1复合物介导糖尿病性心房重塑的内质网应激-线粒体氧化应激。  |  Yuan, M., et al. 2022. Redox Biol. 52: 102289. PMID: 35344886
  4. GRP75-纤毛线粒体相关ER膜（MAM）完整性控制卵巢癌患者的顺铂耐药性  |  Li, J., et al. 2022. Int J Biol Sci. 18: 2914-2931. PMID: 35541901
  5. TGR5 通过抑制 GRP75 介导的糖尿病视网膜病变内质网-线粒体耦合，抑制 cGAS/STING 通路。  |  Li, Y., et al. 2023. Cell Death Dis. 14: 583. PMID: 37658045
  6. 用氯丙嗪衍生物靶向 GRP75 可通过 GRP75-IP3R-Ca2+-AMPK 轴抑制子宫内膜癌进展  |  Wang, Q., et al. 2024. Adv Sci (Weinh). 11: e2304203. PMID: 38342610
  7. GRP75 触发白色脂肪组织褐变，促进癌症相关恶病质的形成。  |  Chen, X., et al. 2024. Signal Transduct Target Ther. 9: 253. PMID: 39327432
  8. 克隆大鼠 grp75（一种 hsp70 家族成员）及其在正常和缺血性脑中的表达。  |  Massa, SM., et al. 1995. J Neurosci Res. 40: 807-19. PMID: 7629893
  9. 真核生物中蛋白质介导的蛋白质成熟。  |  Haas, IG. 1995. FEBS Lett. 369: 72-5. PMID: 7641888
  10. Hsp70 蛋白能充当产生力的马达吗？  |  Glick, BS. 1995. Cell. 80: 11-4. PMID: 7813006
  11. 人类线粒体 hsp70 的克隆和亚细胞定位。  |  Bhattacharyya, T., et al. 1995. J Biol Chem. 270: 1705-10. PMID: 7829505
  12. grp94（内质蛋白）的自身磷酸化。  |  Csermely, P., et al. 1995. J Biol Chem. 270: 6381-8. PMID: 7890776

订购信息

产品名称产品编号规格价格数量收藏夹

GRP 75 抗体 (D-9)

sc-133137
200 µg/ml
$316.00

GRP 75 (D-9): m-IgGκ BP-HRP 套装

sc-521247
200 µg Ab, 40 µg BP
$354.00

GRP 75 (D-9): m-IgG1 BP-HRP 套装

sc-533486
200 µg Ab; 20 µg BP
$354.00

GRP 75 抗体 (D-9) AC

sc-133137 AC
500 µg/ml, 25% agarose
$416.00

GRP 75 抗体 (D-9) HRP

sc-133137 HRP
200 µg/ml
$316.00

GRP 75 抗体 (D-9) FITC

sc-133137 FITC
200 µg/ml
$330.00

GRP 75 抗体 (D-9) PE

sc-133137 PE
200 µg/ml
$343.00

GRP 75 抗体 (D-9) Alexa Fluor® 488

sc-133137 AF488
200 µg/ml
$357.00

GRP 75 抗体 (D-9) Alexa Fluor® 546

sc-133137 AF546
200 µg/ml
$357.00

GRP 75 抗体 (D-9) Alexa Fluor® 594

sc-133137 AF594
200 µg/ml
$357.00

GRP 75 抗体 (D-9) Alexa Fluor® 647

sc-133137 AF647
200 µg/ml
$357.00

GRP 75 抗体 (D-9) Alexa Fluor® 680

sc-133137 AF680
200 µg/ml
$357.00

GRP 75 抗体 (D-9) Alexa Fluor® 790

sc-133137 AF790
200 µg/ml
$357.00

Do you have an isotype control for this antibody?

Asked by: Flow
Thank you for your question. The isotype control corresponding to this antibody would be normal mouse IgG1: sc-3877.
Answered by: Tech Support Europe
Date published: 2022-11-30

Can GRP 75 (D-9): sc-133137 monoclonal antibody be used in IHC with paraffin-embedded samples?

Asked by: jenni
Thank you for your question. Yes, GRP 75 (D-9): sc-133137 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 6
Date published: 2017-01-10
  • y_2025, m_8, d_28, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_133137, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 118ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Amazing!!I requested a free sample of this antibody and I tried it on western blot... It works perfectly!
Date published: 2020-12-21
Rated 4 out of 5 by from works OkWorks well for mice and human samples. however, with same brain samples, we sometimes got non-replicable results
Date published: 2019-10-15
Rated 4 out of 5 by from Funzionamento buono!L'anticorpo anti GRP 75 sc-133137 (Lot #A3119) è stato testato su 2 e 5 µg di lisato proteico, estratto da mitocondri provenienti da cellule HeLa. Come si può notare dall'immagine del Western Blotting allegata, l' anticorpo fornisce un segnale intenso e corrispondente al peso molecolare della proteina da esso riconosciuta.
Date published: 2019-10-03
Rated 5 out of 5 by from It works very well in westernI use 1:500 in western blot,the band is clear,the Molecular Weight is 72-95kd of HUVECs,it is a good antibody.I like santa.
Date published: 2018-03-09
Rated 5 out of 5 by from good antibodyWorking in hek cells 1:100 concentration for WB perfectly
Date published: 2018-02-20
Rated 5 out of 5 by from Nicely working antibodyI received a sample of this antibody and tested it against C. elegans lysates. It works very nicely. Gives strong band for total lysates (it recognizes mitochondrial protein), I used 1:100 dilution
Date published: 2018-02-14
Rated 5 out of 5 by from Very goodUsed for WB on HCT116 cell lysate at a 1:500 dilution in 1%BSA/TBS-T. Unique and strong band at expected size (75 kDa) after few seconds of exposure.
Date published: 2017-10-18
Rated 5 out of 5 by from Super anticorpsFonctionne très bien en WB. Testé au 1/200 sur extraits totaux de cellules COV434. Une seule bande visible au bon poids moléculaire. Bande très intense dès 1 s d'exposition.
Date published: 2017-10-11
  • y_2025, m_8, d_29, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_25
  • loc_zh_CN, sid_133137, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 8ms
  • REVIEWS, PRODUCT
GRP 75 抗体 (D-9) is rated 4.6 out of 5 by 25.
  • y_2025, m_8, d_29, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_25
  • loc_zh_CN, sid_133137, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 106ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。