GRK 5 Double Nickase Plasmid (m): sc-420661-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GRK 5 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GRK 5 Double Nickase Plasmid (m) and GRK 5 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Grk5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GRK 5 Antibody (D-9): sc-518005
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GRK 5 Double Nickase Plasmid (m) | sc-420661-NIC | 20 µg | $410.00 |
Mouse Grk5 encodes G protein-coupled receptor kinase 5 (GRK5), a serine/threonine kinase that phosphorylates activated GPCRs to initiate β-arrestin recruitment, receptor desensitization, and internalization. Through this role, GRK5 helps tune signaling amplitude and duration across pathways downstream of adrenergic, muscarinic, and chemokine receptors, influencing second-messenger dynamics and downstream transcriptional programs. GRK5 activity also intersects with broader regulatory processes including membrane trafficking and signal compartmentalization, and has been linked in the literature to cardiovascular signaling, inflammatory responses, and neuronal GPCR regulation. These connections make Grk5 a useful target for dissecting GPCR feedback control and mapping pathway rewiring under stress or disease-relevant stimuli in mouse model systems.
GRK 5 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Grk5 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Grk5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Grk5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Grk5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.