GRK 3 Double Nickase Plasmid (h): sc-403415-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GRK 3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GRK 3 Double Nickase Plasmid (h) and GRK 3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GRK3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GRK 3 Antibody (C-11): sc-365197
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GRK 3 Double Nickase Plasmid (h) | sc-403415-NIC | 20 µg | $410.00 | |||
GRK 3 Double Nickase Plasmid (h2) | sc-403415-NIC-2 | 20 µg | $410.00 |
GRK3 (also known as ADRBK2) encodes G protein-coupled receptor kinase 3, a serine/threonine kinase that phosphorylates activated GPCRs to promote β-arrestin recruitment, receptor desensitization, and endocytic trafficking. Through this canonical GRK–arrestin axis, GRK3 modulates the intensity and duration of downstream second-messenger signaling, including cAMP/PKA and MAPK/ERK pathways, and can influence chemokine receptor and neurotransmitter receptor responses. GRK3 activity integrates into broader networks controlling signal termination, receptor resensitization, and biased signaling decisions. Dysregulated GPCR desensitization and altered GRK3 expression or function have been investigated in the context of neuropsychiatric phenotypes, immune-cell migration, and cancer-associated signaling rewiring, supporting its relevance for pathway-focused mechanistic studies.
GRK 3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GRK3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GRK3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GRK3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GRK3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.