Date published: 2025-9-8

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granzyme K 抗体 (GM6C3): sc-56125

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说明书
  • granzyme K 抗体 GM6C3 是小鼠单克隆 IgG2b κ,granzyme K抗体, 在25篇文献中引用,规格为200 µg/ml
  • 免疫human物种的granzyme K
  • granzyme K 抗体 (GM6C3) 推荐用于FCM,检测human 来源的 granzyme K transiently expressed on the cell surface of transfected BOSC cells as well as the native protein in peripheral blood mononuclear cells
  • 抗granzyme K抗体(GM6C3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素偶联抗体,可用于WB、IHC(P)和ELISA实验
  • 关于如何获取granzyme K (GM6C3): sc-56125免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2b BP-HRP">m-IgG2b BP-HRP是granzyme K Antibody (GM6C3). 的首选辅助检测试剂,这些试剂现与granzyme K Antibody (GM6C3) 打包提供(请参阅下面的订购信息)。

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関連項目

颗粒酶K抗体(GM6C3)是一种小鼠单克隆IgG2b kappa轻链抗体,可通过流式细胞术(FCM)检测人类颗粒酶K。颗粒酶K抗体(GM6C3)有非结合 以及各种结合形式,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物,可满足不同实验需求。颗粒酶K(Granzyme K)也称为GMZK或NK-tryptase-2,是一种丝氨酸蛋白酶,在免疫反应中起着至关重要的作用,特别是在自然杀伤细胞和细胞毒性T淋巴细胞的颗粒中。GMZK主要在胸腺、肺、脾脏和外周血白细胞中表达,在细胞介导的免疫反应中,GMZK有助于靶细胞的裂解。GMZK包含一个肽酶S1结构域,该结构域对于酶活性至关重要,使GMZK能够切割特定的底物,并诱导感染或恶性细胞凋亡。了解颗粒酶K的功能至关重要,因为颗粒酶K不仅有助于消除有害细胞,而且有助于深入了解免疫调节机制和增强各种疾病免疫反应的潜在治疗靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

granzyme K 抗体 (GM6C3) 参考文献:

  1. 从大肠杆菌包涵体中生成具有催化活性的粒酶 K,并鉴定人体血浆中有效的粒酶 K 抑制剂。  |  Wilharm, E., et al. 1999. J Biol Chem. 274: 27331-7. PMID: 10480954
  2. 人类原粒酶 K 的 2.2-A 晶体结构揭示了一种具有不寻常特征的刚性酶原。  |  Hink-Schauer, C., et al. 2002. J Biol Chem. 277: 50923-33. PMID: 12384499
  3. 纯化三种细胞毒性淋巴细胞颗粒丝氨酸蛋白酶,它们通过不同的底物和靶细胞相互作用诱导细胞凋亡。  |  Shi, L., et al. 1992. J Exp Med. 176: 1521-9. PMID: 1460416
  4. 从分子角度理解氧张力和患者可变性对体内外扩增 T 细胞的影响。  |  Haddad, H., et al. 2004. Biotechnol Bioeng. 87: 437-50. PMID: 15286980
  5. 在大肠杆菌中表达具有酶活性的人类颗粒酶 3,以分析其底物特异性。  |  Hirata, Y., et al. 2006. Arch Biochem Biophys. 446: 35-43. PMID: 16405860
  6. 从人类 IL-2 激活的杀伤细胞中分离出的三种丝氨酸酯酶的特征。  |  Hameed, A., et al. 1988. J Immunol. 141: 3142-7. PMID: 3262682
  7. 克隆人类颗粒酶 3 的 cDNA。  |  Przetak, MM., et al. 1995. FEBS Lett. 364: 268-71. PMID: 7758581
  8. 第二种人类自然杀伤细胞颗粒胰蛋白酶 HNK-Tryp-2/granzyme 3 的克隆与表达。  |  Sayers, TJ., et al. 1996. J Leukoc Biol. 59: 763-8. PMID: 8656064

订购信息

产品名称产品编号规格价格数量收藏夹

granzyme K 抗体 (GM6C3)

sc-56125
200 µg/ml
$316.00

granzyme K (GM6C3): m-IgG Fc BP-HRP 套装

sc-525648
200 µg Ab; 10 µg BP
$354.00

granzyme K (GM6C3): m-IgG2b BP-HRP 套装

sc-549604
200 µg Ab; 10 µg BP
$354.00

granzyme K 抗体 (GM6C3) AC

sc-56125 AC
500 µg/ml, 25% agarose
$416.00

granzyme K 抗体 (GM6C3) HRP

sc-56125 HRP
200 µg/ml
$316.00

granzyme K 抗体 (GM6C3) FITC

sc-56125 FITC
200 µg/ml
$330.00

granzyme K 抗体 (GM6C3) PE

sc-56125 PE
200 µg/ml
$343.00

granzyme K 抗体 (GM6C3) Alexa Fluor® 488

sc-56125 AF488
200 µg/ml
$357.00

granzyme K 抗体 (GM6C3) Alexa Fluor® 546

sc-56125 AF546
200 µg/ml
$357.00

granzyme K 抗体 (GM6C3) Alexa Fluor® 594

sc-56125 AF594
200 µg/ml
$357.00

granzyme K 抗体 (GM6C3) Alexa Fluor® 647

sc-56125 AF647
200 µg/ml
$357.00

granzyme K 抗体 (GM6C3) Alexa Fluor® 680

sc-56125 AF680
200 µg/ml
$357.00

granzyme K 抗体 (GM6C3) Alexa Fluor® 790

sc-56125 AF790
200 µg/ml
$357.00

granzyme K 抗体 (GM6C3) B

sc-56125 B
200 µg/ml
$320.00

Does SCBT offer custom antibody conjugations? we would be interested in a biotinylated format

Asked by: kpaj
Thank you for your question.To request a biotinylated form please email specialorders@scbt.com.
Answered by: BlakeJ
Date published: 2024-03-21

Do you offer this as a biotinylated antibody?

Asked by: Anna
Thank you for your question.To request a biotinylated form please email specialorders@scbt.com.
Answered by: BlakeJ
Date published: 2024-03-21

Hello, I want to integrate Anti-granzyme K Antibody (GM6C3): sc-56125 to my mass cytometry panel. I saw that there is gellatin in the composition. Do you thin that it is a problem for coupling this antibody with metals? Thank you in advance Naoill

Asked by: Naoill
Thank you for your question. Gelatin will negatively impact the efficiency of conjugation kits or experiments. Please contact our technical service directly, then we can issue a quote for a custom formulation without gelatin for this antibody. Thanks!
Answered by: Tech Support Europe
Date published: 2021-11-22

It says the concentration is 200ug/ml but what volume does the antibody come in?

Asked by: perri
Thank you for your question. Granzyme K (GM6C3): sc-56125 is provided at 200 μg IgG2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support
Date published: 2018-12-05

Can granzyme K (GM6C3): sc-56125 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
  • y_2025, m_8, d_27, h_5CST
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Rated 5 out of 5 by from Good for FCMThe granzyme K Antibody (GM6C3): sc-56125 works well for indirect Flow Cytometry applications. This antibody has also been cited for further verification.
Date published: 2017-01-27
Rated 4 out of 5 by from Clear indirect FCM analysis of BOCS23 cellsClear indirect FCM analysis of BOCS23 cells stained with granzyme K . -SCBT QC
Date published: 2013-01-23
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granzyme K 抗体 (GM6C3) is rated 4.5 out of 5 by 2.
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