Grancalcin Antibody (D-12): sc-393792

Grancalcin Antibody (D-12) is a mouse monoclonal IgG1 antibody that detects Grancalcin in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Grancalcin is a calcium-binding protein predominantly found in human neutrophils and is a member of the penta EF-hand (PEF) subfamily of EF-hand proteins, which includes calpain, sorcin, peflin, and ALG-2. Grancalcin undergoes significant conformational changes upon calcium binding, exposing hydrophobic amino acid residues that facilitate interaction with various hydrophobic surfaces within the cell. Grancalcin forms a homodimer through the association of unpaired EF5 hands, enhancing stability and functional capacity. Grancalcin′s interaction with L-plastin, a protein recognized for actin bundling activity, suggests a vital role in regulating neutrophil adhesion, essential for immune response and inflammation. Grancalcin is primarily associated with cells derived from the bone marrow, exhibiting high abundance in neutrophils and monocytes, while only trace amounts are found in lymphocytes, underscoring significance in innate immunity and cellular signaling pathways. Anti-Grancalcin antibody (D-12) serves as an invaluable tool for researchers investigating neutrophil function dynamics and immune response mechanisms.

The preferred Grancalcin antibody is Grancalcin Antibody (G-3): sc-365808.