Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
GPR84 Double Nickase Plasmid (h): sc-401953-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR84 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GPR84 Double Nickase Plasmid (h) and GPR84 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GPR84. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: GPR84 Antibody (1D9): sc-293447
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR84 Double Nickase Plasmid (h) | sc-401953-NIC | 20 µg | $410.00 |
GPR84 encodes a rhodopsin-like G protein–coupled receptor that is activated by medium-chain fatty acids and couples predominantly to Gi/o signaling to modulate cAMP and downstream inflammatory programs. In myeloid and other immune-responsive cell types, GPR84 influences chemotaxis, cytokine output, and metabolic-inflammation crosstalk through pathways that converge on MAPK and NF-κB-dependent transcriptional regulation. Altered GPR84 activity has been associated with dysregulated innate immune responses and inflammatory microenvironments in multiple disease contexts, supporting its use as a molecular handle for studying immunometabolic signaling. As a surface receptor integrating lipid-derived cues, GPR84 is also relevant for dissecting how nutrient availability shapes immune cell state and tissue remodeling.
GPR84 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GPR84 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GPR84. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GPR84 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GPR84-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.