GPR174 Double Nickase Plasmid (h): sc-413678-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR174 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GPR174 Double Nickase Plasmid (h) and GPR174 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GPR174. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR174 Double Nickase Plasmid (h) | sc-413678-NIC | 20 µg | $410.00 | |||
GPR174 Double Nickase Plasmid (h2) | sc-413678-NIC-2 | 20 µg | $410.00 |
GPR174 encodes a G protein-coupled receptor expressed prominently in immune cell subsets, where it contributes to signal transduction that shapes T cell activation, cytokine production, and immune homeostasis. As a membrane receptor, GPR174 engages heterotrimeric G proteins to modulate second-messenger pathways such as cAMP/PKA signaling and downstream transcriptional programs that influence cell state and trafficking. Dysregulated GPCR signaling in lymphocytes is relevant to inflammatory and autoimmune biology, and altered GPR174 activity has been linked to immune phenotype variation in genetic studies. Accordingly, GPR174 serves as a useful node for investigating receptor-mediated control of adaptive immune responses and lymphocyte functional differentiation.
GPR174 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GPR174 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GPR174. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GPR174 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GPR174-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.