GPR105 Double Nickase Plasmid (m): sc-431244-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPR105 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GPR105 Double Nickase Plasmid (m) and GPR105 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting P2ry14. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPR105 Double Nickase Plasmid (m) | sc-431244-NIC | 20 µg | $410.00 | |||
GPR105 Double Nickase Plasmid (m2) | sc-431244-NIC-2 | 20 µg | $410.00 |
Mouse P2ry14 encodes the G protein–coupled receptor GPR105 (P2Y14), a nucleotide sugar receptor that is activated by UDP-sugars such as UDP-glucose and couples primarily to Gi signaling. GPR105 engagement can modulate cAMP levels and influence downstream pathways that shape chemotaxis, cytokine production, and innate immune activation, with reported roles in myeloid cell function and barrier-associated inflammation. In murine systems, P2ry14 activity has been linked to inflammatory responses and metabolic stress signaling, making it relevant to studies of airway and intestinal inflammation, adipose tissue immunometabolism, and microglia-associated neuroinflammatory processes. As a GPCR positioned at the interface of extracellular nucleotide metabolism and immune signaling, P2ry14 is frequently used to interrogate purinergic regulation of tissue homeostasis and disease-relevant inflammatory circuits.
GPR105 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the P2ry14 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within P2ry14. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt P2ry14 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of P2ry14-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.