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Golgi Marker 抗体 (AE-6): sc-58770

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说明书
  • Golgi Marker 抗体 AE-6 是小鼠单克隆 IgG1 κ，Golgi Marker抗体, 在9篇文献中引用，规格为200 µg/ml
  • 针对human来源的SU-DHL-1大细胞淋巴瘤
  • Golgi Marker 抗体 (AE-6) 推荐用于IF, IHC(P) 和 FCM，检测human 来源的 Golgi Zone
  • 抗Golgi Marker抗体（AE-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Golgi Marker (AE-6): sc-58770的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是Golgi Marker Antibody (AE-6) for IHC(P) applications. 的首选辅助检测试剂，这些试剂现与Golgi Marker Antibody (AE-6) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

高尔基体标记物抗体（AE-6）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）检测人类高尔基体标记物蛋白。高尔基体标记物 (AE-6) 抗体有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。高尔基体标记蛋白在细胞加工和蛋白质与脂质的运输过程中起着至关重要的作用，因此高尔基体标记蛋白对维持细胞的平衡和功能至关重要。高尔基体标记蛋白位于高尔基体内，参与内质网中合成的大分子的修饰、分拣和包装，然后被分送到细胞内外的最终目的地。高尔基体的结构是一系列扁平的、与膜结合的囊泡，被称为 "囊泡"（cisternae），分为三个功能区：顺面（cis face）、内侧（medial region）和反面（trans face）。每个区域都含有特定的酶，可促进蛋白质成熟所需的翻译后修饰，如糖基化和磷酸化，这对蛋白质的稳定性和活性至关重要。高尔基体在分泌细胞（如浆 B 细胞）中尤为突出，浆 B 细胞中的高尔基体标记蛋白负责抗体的产生。了解高尔基体的动态和标记对于研究细胞过程、疾病机制和治疗靶点的研究人员来说至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Golgi Marker 抗体 (AE-6) 参考文献:

  1. 利用新型高尔基体标记 PfGRASP 重新定义恶性疟原虫的高尔基体复合体。  |  Struck, NS., et al. 2005. J Cell Sci. 118: 5603-13. PMID: 16306223
  2. 高尔基体中的 H+ 转移 ATP 酶。作为液泡 H+-ATPase 的特征及其亚基结构。  |  Moriyama, Y. and Nelson, N. 1989. J Biol Chem. 264: 18445-50. PMID: 2530222
  3. 大鼠肝癌细胞中高尔基体标记物硫胺素焦磷酸酶的动力学分布受溴苯胍 A 的调节。  |  Fujiwara, T., et al. 1989. Cell Struct Funct. 14: 605-16. PMID: 2559814
  4. 高尔基体各隔室之间蛋白质转运的特征：无细胞系统中供体和受体活动的不对称特性。  |  Balch, WE. and Rothman, JE. 1985. Arch Biochem Biophys. 240: 413-25. PMID: 2990347
  5. 豚鼠睾丸的双睾丸样结构缺乏半乳糖基转移酶，而半乳糖基转移酶是高尔基体的标记。  |  Morré, DJ., et al. 1985. Cell Tissue Res. 240: 35-40. PMID: 3922620
  6. 雄性黑线大鼠肝细胞结节中内质网和高尔基体的分离与鉴定  |  Eriksson, LC., et al. 1983. Cancer Res. 43: 3335-47. PMID: 6189597
  7. 糖核苷酸进入大鼠肝脏高尔基体的运输。一种新的高尔基体标记活性  |  Sommers, LW. and Hirschberg, CB. 1982. J Biol Chem. 257: 10811-7. PMID: 7050120
  8. 恶性疟原虫将高尔基体标记物鞘磷脂合成酶输出到成熟红细胞胞质中的管状网络中。  |  Elmendorf, HG. and Haldar, K. 1994. J Cell Biol. 124: 449-62. PMID: 8106545
  9. 参与从高尔基体到内质网的囊泡运输的新驱动蛋白 KIF1C 的特征。  |  Dorner, C., et al. 1998. J Biol Chem. 273: 20267-75. PMID: 9685376

订购信息

产品名称产品编号规格价格数量收藏夹

Golgi Marker 抗体 (AE-6)

sc-58770
200 µg/ml
$316.00

Golgi Marker (AE-6): m-IgG Fc BP-HRP 套装

sc-526914
200 µg Ab; 10 µg BP
$354.00

Golgi Marker (AE-6): m-IgG1 BP-HRP 套装

sc-532287
200 µg Ab; 20 µg BP
$354.00

Golgi Marker 抗体 (AE-6) AC

sc-58770 AC
500 µg/ml, 25% agarose
$416.00

Golgi Marker 抗体 (AE-6) HRP

sc-58770 HRP
200 µg/ml
$316.00

Golgi Marker 抗体 (AE-6) FITC

sc-58770 FITC
200 µg/ml
$330.00

Golgi Marker 抗体 (AE-6) PE

sc-58770 PE
200 µg/ml
$343.00

Golgi Marker 抗体 (AE-6) Alexa Fluor® 488

sc-58770 AF488
200 µg/ml
$357.00

Golgi Marker 抗体 (AE-6) Alexa Fluor® 546

sc-58770 AF546
200 µg/ml
$357.00

Golgi Marker 抗体 (AE-6) Alexa Fluor® 594

sc-58770 AF594
200 µg/ml
$357.00

Golgi Marker 抗体 (AE-6) Alexa Fluor® 647

sc-58770 AF647
200 µg/ml
$357.00

Golgi Marker 抗体 (AE-6) Alexa Fluor® 680

sc-58770 AF680
200 µg/ml
$357.00

Golgi Marker 抗体 (AE-6) Alexa Fluor® 790

sc-58770 AF790
200 µg/ml
$357.00

Which Golgi protein/s does this antibody target? I am working a cell line in which a key Golgi protein is deleted and this deletion affects several other proteins.

Asked by: ZeDe
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2024-04-29

Hello. I am searching for an anti-mouse antibody to use as Golgi marker in mouse fibroblasts in vitro for IF experiments. I will be grateful if you indicate to me which is the best option. Thank you in advance. Paula

Asked by: Paula5
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

Can Golgi Marker (AE-6): sc-58770 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Golgi Marker (AE-6): sc-58770 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good antibodyUsed this antibody for immunofluorescence in HEK 293 cells- produced weak but detectable perinuclear-cytoplasmic punctae-like staining when used at 1:20 dilution. Staining was not too strong and so was detectable only on a good confocal microscope and not regular fluorescence microscope.
Date published: 2016-10-27
Rated 4 out of 5 by from This antibody was used in a publication forThis antibody was used in a publication for IF on human C4-2 prostate cancer cells, A431 cell and Ishikawa cells - SCBT Publication Review
Date published: 2015-02-19
Rated 4 out of 5 by from Publishable WB data with human MELAS fibroblastsPublishable WB data with human MELAS fibroblasts - SCBT Publication Review
Date published: 2013-07-18
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Golgi Marker 抗体 (AE-6) is rated 4.0 out of 5 by 3.
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