GM130 Antibody (NN 2C10/1): sc-53296
- GM130 Antibody (NN 2C10/1) is a mouse monoclonal IgG2a, cited in 3 publications, provided at 200 µg/ml
- raised against recombinant GM130 of rat origin
- recommended for detection of GM130 of mouse and rat origin by WB, IP, IF and IHC(P)
- See GM130 (H-7): sc-55590 for GM130 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for GM130 Antibody (NN 2C10/1). This work is in progress.
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GM130 Antibody (NN 2C10/1) is a mouse monoclonal IgG2a antibody that detects GM130 in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. GM130 plays a crucial role in the organization and function of the Golgi apparatus, serving as a cis-Golgi matrix protein essential for docking transport vesicles to target membranes. GM130′s amino-terminus specifically binds to p115, facilitating the formation of a membrane docking site, while GM130′s carboxy-terminus anchors to Golgi membranes. This interaction is vital for vesicle tethering, a key step in protein transport from the endoplasmic reticulum to Golgi compartments. GM130 undergoes mitotic phosphorylation at Serine 25 by Cdc2, disrupting p115 binding and contributing to Golgi fragmentation during mitosis. This phosphorylation occurs in prophase and persists through metaphase and anaphase, with dephosphorylation by PP2A in telophase marking Golgi reassembly. Dynamic regulation of GM130 through phosphorylation highlights GM130′s importance in maintaining cellular organization during cell division, making anti-GM130 antibody (NN 2C10/1) an invaluable tool for studying Golgi function and dynamics in various biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GM130 Antibody (NN 2C10/1) References:
- The role of the tethering proteins p115 and GM130 in transport through the Golgi apparatus in vivo. | Seemann, J., et al. 2000. Mol Biol Cell. 11: 635-45. PMID: 10679020
- Identification of the interaction of VP1 with GM130 which may implicate in the pathogenesis of CVB3-induced acute pancreatitis. | Li, X., et al. 2015. Sci Rep. 5: 13324. PMID: 26314804
- Herpes Simplex Virus 1-Induced Blood-Brain Barrier Damage Involves Apoptosis Associated With GM130-Mediated Golgi Stress. | He, Q., et al. 2020. Front Mol Neurosci. 13: 2. PMID: 32038167
- Loss of GM130 does not impair oocyte meiosis and embryo development in mice. | Jiang, Y., et al. 2020. Biochem Biophys Res Commun. 532: 336-340. PMID: 32873390
- The Role of GM130 in Nervous System Diseases. | Huang, B., et al. 2021. Front Neurol. 12: 743787. PMID: 34777211
- GM130 protects against blood-brain barrier disruption and brain injury after intracerebral hemorrhage by regulating autophagy formation. | Deng, S., et al. 2022. Exp Gerontol. 163: 111772. PMID: 35331826
- Associations of IFT20 and GM130 protein expressions with clinicopathological features and survival of patients with lung adenocarcinoma. | Li, L., et al. 2022. BMC Cancer. 22: 809. PMID: 35869490
- Generation of GM130 Conditional Knockout Mouse. | Mei, M. and Bao, S. 2023. Methods Mol Biol. 2557: 61-81. PMID: 36512210
- WDR38, a novel equatorial segment protein, interacts with the GTPase protein RAB19 and Golgi protein GM130 to play roles in acrosome biogenesis. | Gao, Q., et al. 2023. Acta Biochim Biophys Sin (Shanghai). 55: 1561-1570. PMID: 37635409
- RNA scaffolds the Golgi ribbon by forming condensates with GM130. | Zhang, Y. and Seemann, J. 2024. Nat Cell Biol. 26: 1139-1153. PMID: 38992139
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GM130 Antibody (NN 2C10/1) | sc-53296 | 200 µg/ml | $322.00 |