Date published: 2025-9-7
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GM130 Antibody (H-7) - Western Blotting - Image 15003
GM130 抗体 (H-7): sc-55590. Hep G2 (A) 和 NIH/3T3 (B) 全细胞裂解液及大鼠肝组织提取物 (C) 中 GM130 表达的 Western 印迹分析.
GM130 Antibody (H-7) - Western Blotting - Image 15003
GM130 抗体 (H-7): sc-55590. Hep G2 (A) 和 NIH/3T3 (B) 全细胞裂解液及大鼠肝组织提取物 (C) 中 GM130 表达的 Western 印迹分析.
GM130 Antibody (H-7) - Immunohistochemistry - Image 17010
GM130 抗体 (H-7): sc-55590. 福尔马林固定, 石蜡包埋的人胃组织的免疫过氧化物酶染色, 低倍 (A) 和高倍 (B) 放大后显示腺细胞的胞浆染色. 瑞典人类蛋白质图谱 (HPA) 计划友情提供.
GM130 Antibody (H-7) - Western Blotting - Image 391197
GM130 抗体 (H-7) Alexa Fluor® 488: sc-55590 AF488. 直接荧光西部印迹分析 HeLa 全细胞裂解液中 GM130 的表达. 用 UltraCruz® 阻断试剂阻断: sc-516214.
GM130 抗体 (H-7): sc-55590. Hep G2 (A) 和 NIH/3T3 (B) 全细胞裂解液及大鼠肝组织提取物 (C) 中 GM130 表达的 Western 印迹分析.

GM130 抗体 (H-7): sc-55590

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说明书
  • GM130 抗体 H-7 是小鼠单克隆 IgG2a κ，GM130抗体, 在25篇文献中引用，规格为200 µg/ml
  • 针对氨基酸191-255的图谱，在GM130的内部区域，human起源
  • GM130 抗体 (H-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 GM130
  • 抗GM130抗体（H-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取GM130 (H-7): sc-55590的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 GM130 抗体 (H-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与GM130 抗体 (H-7) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

GM130抗体（H-7）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类的GM130。GM130 (H-7) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。GM130是一种重要的顺式高尔基基质蛋白，在组织和维持高尔基体的功能方面发挥着至关重要的作用，对于细胞内蛋白质和脂质的正常处理和分类至关重要。GM130的结构包括一个独特的氨基端，可与p115蛋白结合，促进运输囊泡与高尔基膜对接，而其羧基端则锚定在高尔基结构上。这种双重结合能力可维持高尔基体的完整性和功能，使GM130能够与Giantin等蛋白一起参与囊泡连接。GM130与p115和Giantin的相互作用对于将物质从内质网运送到高尔基体至关重要，凸显了GM130在细胞运输过程中的重要性。GM130经历翻译后修饰，例如有丝分裂期间的磷酸化，从而调节功能和相互作用，进一步强调了GM130在细胞动力学中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GM130 抗体 (H-7) 参考文献:

  1. 系链蛋白 p115 和 GM130 在体内通过高尔基体的运输过程中的作用。  |  Seemann, J., et al. 2000. Mol Biol Cell. 11: 635-45. PMID: 10679020
  2. 膜拴系成分的结合关系。giantin N末端和GM130 N末端竞争性地与p115 C末端结合。  |  Linstedt, AD., et al. 2000. J Biol Chem. 275: 10196-201. PMID: 10744704
  3. 顺式高尔基基质蛋白 GM130 的有丝分裂磷酸化周期  |  Lowe, M., et al. 2000. J Cell Biol. 149: 341-56. PMID: 10769027
  4. p115 活性蛋白 GM130 和 giantin 参与了内质网-高尔基体的运输。  |  Alvarez, C., et al. 2001. J Biol Chem. 276: 2693-700. PMID: 11035033
  5. 确定哺乳动物新型 Sec23p 相互作用蛋白 p125 的功能区。  |  Mizoguchi, T., et al. 2000. Biochem Biophys Res Commun. 279: 144-9. PMID: 11112430
  6. GM130是一种顺式高尔基体蛋白，它能调节小鼠卵母细胞减数分裂纺锤体的组装和不对称分裂。  |  Zhang, CH., et al. 2011. Cell Cycle. 10: 1861-70. PMID: 21552007
  7. GM130 是一种平行四聚物，具有柔性杆状结构，N 端呈开放（Y 型）和终止子（I 型）构象。  |  Ishida, R., et al. 2015. FEBS J. 282: 2232-44. PMID: 25787021
  8. 乳腺癌细胞中 GM130 的缺失及其对细胞迁移、侵袭和极性的影响。  |  Baschieri, F., et al. 2015. Cell Cycle. 14: 1139-47. PMID: 25892554
  9. GM130 通过蜗牛调节上皮细胞向间质转化和胃癌细胞的侵袭。  |  Zhao, J., et al. 2015. Int J Clin Exp Pathol. 8: 10784-91. PMID: 26617790
  10. GM130 可调节肺泡 II 型细胞中肺部表面活性蛋白的分泌。  |  Pang, Q., et al. 2022. Sci China Life Sci. 65: 193-205. PMID: 33740186
  11. 囊泡对接蛋白p115以一种有丝分裂调控方式与顺式高尔基基质蛋白GM130结合。  |  Nakamura, N., et al. 1997. Cell. 89: 445-55. PMID: 9150144
  12. Cdc2 激酶直接磷酸化顺式高尔基体基质蛋白 GM130，是有丝分裂过程中高尔基体破碎所必需的。  |  Lowe, M., et al. 1998. Cell. 94: 783-93. PMID: 9753325

订购信息

产品名称产品编号规格价格数量收藏夹

GM130 抗体 (H-7)

sc-55590
200 µg/ml
$316.00

GM130 (H-7): m-IgG Fc BP-HRP 套装

sc-525644
200 µg Ab; 10 µg BP
$354.00

GM130 (H-7): m-IgG2a BP-HRP 套装

sc-546351
200 µg Ab; 10 µg BP
$354.00

GM130 抗体 (H-7) AC

sc-55590 AC
500 µg/ml, 25% agarose
$416.00

GM130 抗体 (H-7) HRP

sc-55590 HRP
200 µg/ml
$316.00

GM130 抗体 (H-7) FITC

sc-55590 FITC
200 µg/ml
$330.00

GM130 抗体 (H-7) PE

sc-55590 PE
200 µg/ml
$343.00

GM130 抗体 (H-7) Alexa Fluor® 488

sc-55590 AF488
200 µg/ml
$357.00

GM130 抗体 (H-7) Alexa Fluor® 546

sc-55590 AF546
200 µg/ml
$357.00

GM130 抗体 (H-7) Alexa Fluor® 594

sc-55590 AF594
200 µg/ml
$357.00

GM130 抗体 (H-7) Alexa Fluor® 647

sc-55590 AF647
200 µg/ml
$357.00

GM130 抗体 (H-7) Alexa Fluor® 680

sc-55590 AF680
200 µg/ml
$357.00

GM130 抗体 (H-7) Alexa Fluor® 790

sc-55590 AF790
200 µg/ml
$357.00

I want a free sample of this product (sc-55590). Do I have to buy another product in order to get the free sample?

Asked by: Lorrie Delehanty
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

I purchased this antibody and used it for western blot ( 1:1000) and (1:500) in 2%milk in TBST but didn't work at a ll. I use whole cells lysate of Hela lysate as a positive control. Can you recommend what concentration to use, please? 

Asked by: Wafaa
Thank you for your question. We recommend using the antibody at a starting dilution of 1:200 in 5% nonfat milk in TBST. However, since you have not observed any signal, we would recommend starting at 1:100. We have not tested the antibody in HeLa whole cell lysate so we do not know about the suitability of this cell line as a positive control for GM130. However, we do recommend using either Hep G2 or NIH/3T3 whole cell lysates, or rat liver tissue extract as a positive control. We recommend loading 50 µg protein per lane. A link to our complete recommended protocol for Western blotting with this antibody is provided below. Please contact our Technical Service Department for additional troubleshooting support. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-13

Can you recommend a positive control to use with GM130 (H-7): sc-55590 monoclonal antibody for Western blot?

Asked by: jenniferc
We recommend Hep G2 (sc-2227) or NIH/3T3 (sc-2210) whole cell lysates to use as positive controls as we have Western blot data showing specific detection of GM130 in these cell lines.
Answered by: Technical Support 2
Date published: 2017-01-11

What secondary is best to use with GM130 (H-7): sc-55590 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with GM130 (H-7): sc-55590, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 2
Date published: 2017-01-11
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Rated 1 out of 5 by from DOES NOT work for IF/ICCWe tested this antibody on HeLa cells using different fixation methods (PFA and MeOH) and dilutions down to 1:50, and different secondary antibodies; however, they DO NOT work for IF/ICC.
Date published: 2023-02-08
Rated 1 out of 5 by from Nessun segnale, altro clone SantaCruz B10 funzionaTestato su campioni di cellule hiPSC e loro vescicole extracellulari (human). Risultato negativo (nessun segnale). Usati 20 ug di proteine totali. Diluizione primario 1:500 in latte 5% PBS-T (0,1% Tween). Invece clone B-10 SC-55591 Anti-GM130 Antibody ha dato segnale sugli stessi campioni.
Date published: 2021-11-30
Rated 1 out of 5 by from did not work in IFThis antibody did not work in IF for Caco-2 cells.
Date published: 2018-05-10
Rated 5 out of 5 by from UPENNGα 12 Antibody is very good. The band is very stronger.
Date published: 2018-04-19
Rated 1 out of 5 by from Does not work in immunofluorescenceI tested this antibody in RPE-1 cells, MetOH fixed, for dilution from 1/100 to 1/500. Does not give a clear signal.
Date published: 2018-03-27
Rated 3 out of 5 by from testé en immunofluorescence et Western Blotj'ai testé cet anticorps sur des cellules MCF7, j'ai obtenue un signal d'intensité correct à une dilution de 1/50, je n'ai pas obtenue de signal en western blot sur des lysats de MCF7
Date published: 2018-02-19
Rated 1 out of 5 by from did not work for IFNo signal in immunofluorescent staining. Used 1:50, overnight (4C), on mouse N2A cells, fixed with 4% PFA. Disappointing.
Date published: 2017-11-22
Rated 1 out of 5 by from No IF signals in mice/rat mandible ameloblastsThis antibody doesn't show bindings on PFA fixed and paraffin embedded sections from mice/rat mandible ameloblasts.
Date published: 2017-07-06
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GM130 抗体 (H-7) is rated 3.3 out of 5 by 17.
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