Glutathione Peroxidase 5/GPX5 Double Nickase Plasmid (h): sc-405279-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Glutathione Peroxidase 5/GPX5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Glutathione Peroxidase 5/GPX5 Double Nickase Plasmid (h) and Glutathione Peroxidase 5/GPX5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GPX5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Glutathione Peroxidase 5/GPX5 Antibody (D-3): sc-390092
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Glutathione Peroxidase 5/GPX5 Double Nickase Plasmid (h) | sc-405279-NIC | 20 µg | $410.00 | |||
Glutathione Peroxidase 5/GPX5 Double Nickase Plasmid (h2) | sc-405279-NIC-2 | 20 µg | $410.00 |
Human GPX5 encodes glutathione peroxidase 5, a selenium-dependent antioxidant enzyme that reduces hydrogen peroxide and lipid hydroperoxides using glutathione to limit oxidative damage. GPX5 activity supports redox homeostasis and helps constrain oxidative stress–driven effects on membrane integrity, protein oxidation, and downstream signaling pathways linked to inflammation and apoptosis. Although GPX5 is best characterized in the male reproductive tract, its regulation and antioxidant function connect it to broader cellular responses to reactive oxygen species and oxidative injury. Dysregulated redox control involving glutathione peroxidases is frequently studied in contexts such as infertility, metabolic stress, and tumor biology where reactive oxygen species influence genomic stability and cell fate decisions.
Glutathione Peroxidase 5/GPX5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GPX5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GPX5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GPX5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GPX5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.