Date published: 2025-9-7

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Giantin 抗体 (2388C3a): sc-81279

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说明书
  • Giantin 抗体 2388C3a 是小鼠单克隆 IgG1 κ,Giantin抗体, 在1篇文献中引用,规格为100 µg/ml
  • 针对与human的Giantin的内部区域相对应的部分重组蛋白
  • 推荐用于 human 来源的Giantin WB 和 IP检测
  • 目前,我们还没有完成Giantin 抗体 (2388C3a)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

Giantin抗体(2388C3a)是一种IgG1 κ小鼠单克隆Giantin抗体(也称为GOGB1抗体、Golgin B1抗体、GCP372抗体、高尔基体自身抗原Golgin B亚家族大Golgin 1抗体、Golgin B1高尔基体整体膜蛋白抗体、372 KDa高尔基体相关蛋白抗体、高尔基体整体膜蛋白1抗体、Golgin B亚家族成员1抗体、大Golgin抗体、GOLIM1或GCP抗体),可通过WB和IP检测人源Giantin蛋白。Giantin抗体(2388C3a)为非偶联抗Giantin抗体。GM130是一种顺式高尔基体基质蛋白,与p115特异性相互作用并提供膜停靠位点。GM130和p115都参与囊泡与高尔基体膜的锚定。蛋白质p115还结合p400,也被称为giantin。Giantin的大部分质量投射到细胞质中,通过p115将COPI囊泡停靠到高尔基体膜上。Giantin也被称为大Golgin或高尔基体相关蛋白,参与高尔基体复合体中的交叉桥形成。Giantin可以形成同源二聚体,它是一种单次跨膜I型膜蛋白,是Sjögren综合征和慢性类风湿性关节炎的抗原。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Giantin 抗体 (2388C3a) 参考文献:

  1. 针对蛋白质运输和信使 RNA 处理途径的自身抗体:内体, 溶酶体, 高尔基复合体, 蛋白酶体, 装配体, 外泌体和 GW 体。  |  Stinton, LM., et al. 2004. Clin Immunol. 110: 30-44. PMID: 14962794
  2. 高尔基体中存在针对巨蛋白的循环自身抗体,并伴有进行性肌病。  |  Sahashi, K., et al. 2004. Neurology. 62: 1891-3. PMID: 15159505
  3. 肌醇多磷酸 5-磷酸酶 Ocrl 与部分包裹有凝集素的内体缔合作用。  |  Ungewickell, A., et al. 2004. Proc Natl Acad Sci U S A. 101: 13501-6. PMID: 15353600
  4. Golgin tethers 界定了 COPI 囊泡的亚群。  |  Malsam, J., et al. 2005. Science. 307: 1095-8. PMID: 15718469
  5. p115 系链蛋白中一个隐秘的 Rab1 结合位点。  |  Beard, M., et al. 2005. J Biol Chem. 280: 25840-8. PMID: 15878873
  6. 基因敲除模型揭示了高尔基体功能与糖基转移酶表达之间的反馈回路。  |  Stevenson, NL., et al. 2017. J Cell Sci. 130: 4132-4143. PMID: 29093022
  7. 在软骨形成过程中,需要 Giantin 来协调生成 aggrecan, 连接蛋白和 XI 型胶原蛋白。  |  Katayama, K., et al. 2018. Biochem Biophys Res Commun. 499: 459-465. PMID: 29577904
  8. 肝细胞高尔基体在酒精作用后的恢复需要 Giantin  |  Casey, CA., et al. 2018. Biomolecules. 8: PMID: 30453527
  9. 高尔基蛋白 Giantin 调节高尔基体堆之间的相互连接  |  Satoh, A., et al. 2019. Front Cell Dev Biol. 7: 160. PMID: 31544102
  10. I 型胶原蛋白的细胞内 N 终止子加工需要 Giantin。  |  Stevenson, NL., et al. 2021. J Cell Biol. 220: PMID: 33944912
  11. 376 千道尔顿高尔基复合体膜蛋白(巨蛋白)的分子遗传分析。  |  Seelig, HP., et al. 1994. Mol Cell Biol. 14: 2564-76. PMID: 7511208
  12. 定位在高尔基复合体中的人 372-kDA 蛋白的分子克隆和序列分析。  |  Sohda, M., et al. 1994. Biochem Biophys Res Commun. 205: 1399-408. PMID: 7802676

订购信息

产品名称产品编号规格价格数量收藏夹

Giantin 抗体 (2388C3a)

sc-81279
100 µg/ml
$333.00

Could you answer the question about the epitope here? What is the range of amino acids used as antigen? Just rough range is fine.

Asked by: Not Maohan
Thank you for your question. The antibody was not epitope mapped . Here is the uniprot page for more information on the protein: https://www.uniprot.org/uniprotkb/Q14789/entry
Answered by: BlakeJ
Date published: 2024-05-02

Could you tell me the epitopes of the antigen? Just rough range is fine. I need to map where the antibody binds at Giantin. Thank you!

Asked by: Not Maohan
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2024-05-01

I am using Giantin (2388C3a): sc-81279 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Randy McDonald
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Good for Western BlotAntibody detects human recombinant Giantin by Western blot. -SCBT QC
Date published: 2023-09-14
Rated 4 out of 5 by from Published immunofluorescence data using HepG2Published immunofluorescence data using HepG2 and MDCK cells. -SCBT Publication Review
Date published: 2015-06-17
Rated 3 out of 5 by from Clean band present in western Blot analysisClean band present in western Blot analysis of human recombinant Giantin fusion protein. -SCBT QC
Date published: 2014-01-08
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Giantin 抗体 (2388C3a) is rated 4.0 out of 5 by 3.
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