Ggta1 CRISPR/Cas9 KO Plasmid (m): sc-420545
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ggta1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Ggta1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ggta1 CRISPR/Cas9 KO Plasmid (m) | sc-420545 | 20 µg | $397.00 |
Overview
Ggta1 encodes alpha-1,3-galactosyltransferase 1, a Golgi-localized glycosyltransferase that catalyzes transfer of galactose to generate the Galα1-3Gal epitope on glycoproteins and glycolipids. By shaping terminal glycan structures, Ggta1 influences protein maturation, membrane glycosylation, and cell–cell or cell–matrix interactions that can affect immune recognition and inflammatory signaling. In mice, Ggta1 activity is relevant to studies of xenoantigen biology and complement/antibody-mediated responses, and it serves as a model for glycosylation-dependent mechanisms in immunity. Disruption of this enzyme is therefore useful for interrogating how glycan epitopes regulate receptor binding, tissue compatibility, and downstream immune pathways.
Ggta1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ggta1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ggta1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ggta1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Ggta1 protein expression.
This CRISPR knockout system enables efficient generation of Ggta1-deficient cell models for investigation of Ggta1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ggta1 exon(s) critical for Ggta1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ggta1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Ggta1 CRISPR/Cas9 KO Plasmid (m) and Ggta1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ggta1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Ggta1 HDR Plasmid (m) and Ggta1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ggta1 homology arms to support homology-directed repair at defined Ggta1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.