GCN2 Antibody (F-7): sc-374609

GCN2 Antibody (F-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects GCN2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). GCN2 Antibody (F-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GCN2, also known as EIF2AK4, is a critical component of the cellular stress response, functioning as a kinase that phosphorylates the eukaryotic translation initiation factor 2α (eIF2α) on Ser 51. This phosphorylation event inhibits the guanine nucleotide exchange factor eIF2B, thereby regulating protein synthesis under stress conditions. GCN2 is particularly responsive to amino acid deprivation and UV radiation, making GCN2 essential for cellular adaptation to environmental stressors. GCN2 is expressed at high levels in liver and brain tissues, highlighting GCN2′s importance in metabolic regulation and neuronal function. The unique structure of GCN2 allows sensing of uncharged tRNA levels, which is crucial for maintaining cellular homeostasis during nutrient scarcity. The interaction of GCN2 with other stress-responsive kinases, such as HRI, PKR, and PERK, underscores GCN2′s integral role in the broader network of stress signaling pathways.

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GCN2 Antibody (F-7) References:

1. Characterization of a mammalian homolog of the GCN2 eukaryotic initiation factor 2alpha kinase.  |  Berlanga, JJ., et al. 1999. Eur J Biochem. 265: 754-62. PMID: 10504407
2. Phosphorylation of the alpha subunit of eukaryotic initiation factor 2 is required for activation of NF-kappaB in response to diverse cellular stresses.  |  Jiang, HY., et al. 2003. Mol Cell Biol. 23: 5651-63. PMID: 12897138
3. Preservation of liver protein synthesis during dietary leucine deprivation occurs at the expense of skeletal muscle mass in mice deleted for eIF2 kinase GCN2.  |  Anthony, TG., et al. 2004. J Biol Chem. 279: 36553-61. PMID: 15213227
4. GCN2 phosphorylation of eIF2alpha activates NF-kappaB in response to UV irradiation.  |  Jiang, HY. and Wek, RC. 2005. Biochem J. 385: 371-80. PMID: 15355306
5. PKR and GCN2 kinases and guanine nucleotide exchange factor eukaryotic translation initiation factor 2B (eIF2B) recognize overlapping surfaces on eIF2alpha.  |  Dey, M., et al. 2005. Mol Cell Biol. 25: 3063-75. PMID: 15798194
6. Translational control of hippocampal synaptic plasticity and memory by the eIF2alpha kinase GCN2.  |  Costa-Mattioli, M., et al. 2005. Nature. 436: 1166-73. PMID: 16121183
7. PERK and GCN2 contribute to eIF2alpha phosphorylation and cell cycle arrest after activation of the unfolded protein response pathway.  |  Hamanaka, RB., et al. 2005. Mol Biol Cell. 16: 5493-501. PMID: 16176978
8. GCN2 drives macrophage and MDSC function and immunosuppression in the tumor microenvironment.  |  Halaby, MJ., et al. 2019. Sci Immunol. 4: PMID: 31836669
9. Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2α axis.  |  Li, C., et al. 2022. Cell Stem Cell. 29: 1119-1134.e7. PMID: 35803229
10. GCN2 inhibition sensitizes arginine-deprived hepatocellular carcinoma cells to senolytic treatment.  |  Missiaen, R., et al. 2022. Cell Metab. 34: 1151-1167.e7. PMID: 35839757
11. The tRNA-GCN2-FBXO22-axis-mediated mTOR ubiquitination senses amino acid insufficiency.  |  Ge, MK., et al. 2023. Cell Metab. 35: 2216-2230.e8. PMID: 37979583
12. Multiple mechanisms activate GCN2 eIF2 kinase in response to diverse stress conditions.  |  Misra, J., et al. 2024. Nucleic Acids Res. 52: 1830-1846. PMID: 38281137

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GCN2 Antibody (F-7) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-GCN2 Antibody (F-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GCN2 Antibody (F-7) Specifications

Alternate Product Name: GCN2

Product Clone Name: F-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1350-1649

Epitope Target: GCN2

Epitope Species: human

Product Reactivity/Detection: GCN2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for GCN2 Antibody (F-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin