Date published: 2026-10-19

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GATA-2 抗体 (H-6): sc-515178

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说明书
  • GATA-2 抗体 H-6 是小鼠单克隆 IgM κ,GATA-2抗体, 在6篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的GATA-2的内部区域内的氨基酸263-287之间
  • 推荐用于 mouse, rat 和 human 来源的GATA-2 WB, IP, IF 和 ELISA检测
  • 另提供琼脂糖偶联抗体,规格为琼脂糖装,可用于IP实验;HRP 偶联抗体用于WB, IHC(P)和ELISA实验;以及藻红蛋白、FITC、Alexa Fluor® 488 或Alexa Fluor® 647 偶联抗体,规格为用于IF、IHC(P)和FCM实验
  • 可用于ChIP的TransCruz试剂(sc-515178 X, 200 µg/0.1 ml)
  • m-IgGκ BP-HRP是GATA-2 Antibody (H-6) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与GATA-2 Antibody (H-6) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。
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関連項目

GATA-2抗体(H-6)是一种小鼠单克隆IgM抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的GATA-2蛋白。抗GATA-2抗体(H-6)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。GATA-2在造血过程中起着至关重要的作用,对于各种血细胞系的发展至关重要,特别是在胚胎发育过程中调节基因表达。GATA-2主要在胚胎脑和肝组织以及内皮细胞和红细胞中表达,这凸显了其在发育和成人造血过程中的重要性。GATA-2与其他GATA家族成员(如GATA-1和GATA-3)相互作用,这些成员参与特定的谱系分化,表明其具有更广泛的调节能力。GATA-2与WGATAR共识序列结合,可以调节对细胞命运决定至关重要的靶基因,使GATA-2成为调控造血干细胞维持和分化的转录网络中的重要组成部分。了解GATA-2的功能和相互作用对于阐明血细胞发育的机制及其在血液疾病中的潜在影响至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GATA-2 抗体 (H-6) 参考文献:

  1. GATA-1/GATA-2转录因子的水平调节胚胎和胎儿血红蛋白的表达。  |  Ikonomi, P., et al. 2000. Gene. 261: 277-87. PMID: 11167015
  2. GATA-2 regulates granulocyte-macrophage progenitor cell function.  |  Rodrigues, NP., et al. 2008. Blood. 112: 4862-73. PMID: 18840712
  3. GATA-2在GATA-1缺失的情况下强化巨核细胞发育。  |  Huang, Z., et al. 2009. Mol Cell Biol. 29: 5168-80. PMID: 19620289
  4. 树突状细胞、单核细胞、B和NK淋巴细胞缺乏定义了新的GATA-2依赖性骨髓增生异常综合征的丢失谱系。  |  Bigley, V. and Collin, M. 2011. Haematologica. 96: 1081-3. PMID: 21810969
  5. GATA-2 通过收费样受体 4/MD88/MAPK 依赖性机制转导 LPS 诱导的巨噬细胞中 il-1β 基因表达。  |  Wu, TT., et al. 2013. PLoS One. 8: e72404. PMID: 23940812
  6. Hyperosmolarity-Induced Down-Regulation of Claudin-2 Mediated by Decrease in PKCβ-Dependent GATA-2 in MDCK Cells.  |  Ikari, A., et al. 2015. J Cell Physiol. 230: 2776-87. PMID: 25825272
  7. 氯喹诺通过下调GATA-2和GATA-3来增加白介素-8的表达。  |  Katsuyama, M., et al. 2018. Neurotoxicology. 67: 296-304. PMID: 29966605
  8. 人类白血病突变会破坏但不会消除GATA-2的功能。  |  Katsumura, KR., et al. 2018. Proc Natl Acad Sci U S A. 115: E10109-E10118. PMID: 30301799
  9. GATA-2缺陷的肥大细胞限制了人类受试者中IgE介导的即时超敏反应。  |  Desai, A., et al. 2019. J Allergy Clin Immunol. 144: 613-617.e14. PMID: 31102699
  10. 急性髓细胞白血病中的GATA 2单基因功能不足:超越显而易见的。  |  Radhakrishnan, N., et al. 2020. Indian Pediatr. 57: 570-571. PMID: 32562402

订购信息

产品名称产品编号规格价格数量收藏夹

GATA-2 抗体 (H-6)

sc-515178
200 µg/ml
$322.00

GATA-2 (H-6): m-IgGκ BP-HRP 套装

sc-552716
200 µg Ab; 40 µg BP
$361.00

GATA-2 抗体 (H-6) AC

sc-515178 AC
500 µg/ml, 25% agarose
$424.00

GATA-2 抗体 (H-6) Alexa Fluor® 488

sc-515178 AF488
200 µg/ml
$364.00

GATA-2 抗体 (H-6) Alexa Fluor® 647

sc-515178 AF647
200 µg/ml
$364.00

GATA-2 抗体 (H-6) FITC

sc-515178 FITC
200 µg/ml
$336.00

GATA-2 抗体 (H-6) HRP

sc-515178 HRP
200 µg/ml
$322.00

GATA-2 (H-6) 中和勝肽

sc-515178 P
100 µg/0.5 ml
$69.00

GATA-2 抗体 (H-6) PE

sc-515178 PE
200 µg/ml
$349.00

GATA-2 抗体 (H-6) X

sc-515178 X
200 µg/0.1 ml
$322.00

What application is the blocking peptide sc-515178 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by Dai Y from Good ICC stainingNice, specific ICC staining with 100% EtOH fixed mouse ECs
Date published: 2023-06-27
Rated 5 out of 5 by sri hari from GATA-2I used for immunofluroscence staining and the antibody worked fine
Date published: 2017-09-22
Rated 1 out of 5 by Ben Copeland from Poor choice for WB in my handsTried a test sample of H-6 in a WB, but the secondary wouldn't pick it up at all. Assuming because it's an IgM. Switched to Mouse anti-GATA2 (sc-267) and it works beautifully, strong distinct bands with no background.
Date published: 2017-06-06
Rated 5 out of 5 by doctor wang from 挺好用用了脐静脉内皮细胞做阳性对照,做蛋白免疫印记,抗体稀释比例大概在1:5000,阳性对照出的条带很清晰,条带分子量的位置也正确,基本上没有杂带,单克隆抗体特异性比较好。因课题内部要求,就不附图了。
Date published: 2017-03-13
Rated 5 out of 5 by cjMara from Validated for WB in different speciesThis monoclonal GATA-2 Antibody (H-6) has been validated in WB using transfected lysates, and for endogenous detection of GATA-2 in mouse, rat and human cell lysates.
Date published: 2017-01-19
Rated 1 out of 5 by dtlo from No supershiftThis antibody did not work in EMSA to super-shift GATA-2 band generated with K562 nuclear extract.
Date published: 2016-11-11
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GATA-2 抗体 (H-6) is rated 3.7 out of 5 by 6.
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