Gas6 CRISPR/Cas9 KO Plasmid (m): sc-420490
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Gas6 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Gas6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Gas6 CRISPR/Cas9 KO Plasmid (m) | sc-420490 | 20 µg | $397.00 |
Overview
Growth arrest–specific 6 (Gas6) is a vitamin K–dependent secreted ligand that engages TAM receptor tyrosine kinases, primarily AXL, MERTK, and TYRO3, to regulate cell survival, proliferation, and migratory responses. Through TAM signaling, Gas6 influences downstream PI3K–AKT, MAPK/ERK, and NF-κB pathways and supports clearance of apoptotic cells via phosphatidylserine-dependent recognition. In mouse systems, Gas6 is implicated in innate immune homeostasis, macrophage efferocytosis, and modulation of inflammatory tone, with relevance to processes including fibrosis, thrombosis, and tumor–immune interactions in the microenvironment.
Gas6 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Gas6 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Gas6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Gas6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Gas6 protein expression.
This CRISPR knockout system enables efficient generation of Gas6-deficient cell models for investigation of Gas6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Gas6 exon(s) critical for Gas6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Gas6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Gas6 CRISPR/Cas9 KO Plasmid (m) and Gas6 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Gas6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Gas6 HDR Plasmid (m) and Gas6 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Gas6 homology arms to support homology-directed repair at defined Gas6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.