GABA_A Rβ3 Antibody (D-12): sc-376252

GABAA Rβ3 Antibody (D-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects GABAA R beta 3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). GABAA Rβ3 monoclonal antibody (D-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GABAA R beta 3 protein plays a crucial role in the central nervous system as a component of the GABAA receptor, which is essential for mediating inhibitory neurotransmission. GABAA R beta 3 is primarily located in the postsynaptic membrane of neurons, where binding with gamma-aminobutyric acid (GABA) facilitates chloride ion influx, leading to neuron hyperpolarization and decreased excitability. Proper functioning of GABAA receptors, including GABAA R beta 3 subunit, is vital for maintaining the balance between excitation and inhibition in the brain, which is critical for various physiological processes such as anxiety regulation, muscle relaxation, and seizure prevention. Dysregulation of GABAA receptor function has been implicated in several neurological disorders, making anti-GABAA Rβ3 antibody (D-12) an important tool for research into these conditions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

GABA_A Rβ3 Antibody (D-12) References:

1. Molecular heterogeneity of the gamma-aminobutyric acid (GABA) transport system. Cloning of two novel high affinity GABA transporters from rat brain.  |  Borden, LA., et al. 1992. J Biol Chem. 267: 21098-104. PMID: 1400419
2. GABA: an excitatory transmitter in early postnatal life.  |  Cherubini, E., et al. 1991. Trends Neurosci. 14: 515-9. PMID: 1726341
3. Cloning of the human brain GABA transporter.  |  Nelson, H., et al. 1990. FEBS Lett. 269: 181-4. PMID: 2387399
4. The GABRB3 Polymorphism and its Association with Schizophrenia.  |  Liu, Y., et al. 2018. J Mol Neurosci. 64: 75-79. PMID: 29196882
5. Structural mapping of GABRB3 variants reveals genotype-phenotype correlations.  |  Johannesen, KM., et al. 2022. Genet Med. 24: 681-693. PMID: 34906499
6. Gabrb3 endothelial cell-specific knockout mice display abnormal blood flow, hypertension, and behavioral dysfunction.  |  Agrud, A., et al. 2022. Sci Rep. 12: 4922. PMID: 35318369
7. Molecular and clinical descriptions of patients with GABAA receptor gene variants (GABRA1, GABRB2, GABRB3, GABRG2): A cohort study, review of literature, and genotype-phenotype correlation.  |  Maillard, PY., et al. 2022. Epilepsia. 63: 2519-2533. PMID: 35718920
8. Gabrb3 is required for the functional integration of pyramidal neuron subtypes in the somatosensory cortex.  |  Babij, R., et al. 2023. Neuron. 111: 256-274.e10. PMID: 36446382
9. Targeting of the 67-kDa isoform of glutamic acid decarboxylase to intracellular organelles is mediated by its interaction with the NH2-terminal region of the 65-kDa isoform of glutamic acid decarboxylase.  |  Dirkx, R., et al. 1995. J Biol Chem. 270: 2241-6. PMID: 7836456
10. GABAC receptors in the vertebrate retina.  |  Lukasiewicz, PD. 1996. Mol Neurobiol. 12: 181-94. PMID: 8884747
11. Expression cloning of GABA(B) receptors uncovers similarity to metabotropic glutamate receptors.  |  Kaupmann, K., et al. 1997. Nature. 386: 239-46. PMID: 9069281
12. GABA(A)-receptor subtypes: clinical efficacy and selectivity of benzodiazepine site ligands.  |  Korpi, ER., et al. 1997. Ann Med. 29: 275-82. PMID: 9375983

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GABA_A Rβ3 Antibody (D-12) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-GABA_A Rβ3 Antibody (D-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GABA_A Rβ3 Antibody (D-12) Specifications

Alternate Product Name: GABAA Rβ3

Product Clone Name: D-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 243-481

Epitope Target: GABA_A Rβ3

Epitope Species: human

Product Reactivity/Detection: GABA_A Rβ3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for GABA_A Rβ3 Antibody (D-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin