Date published: 2025-9-8
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G6PD Antibody (G-12) - Western Blotting - Image 402012
G6PD 抗体 (G-12) Alexa Fluor® 647: sc-373886 AF647. 对 MCF7 (A), A549 (B), SK-BR-3 (C) 和 HeLa (D) 全细胞裂解液及人睾丸组织提取物 (E) 中 G6PD 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker™ MW Tag-Alexa Fluor® 488 检测 Cruz Marker™ 分子量标准: sc-516790.
G6PD 抗体 (G-12) Alexa Fluor® 647: sc-373886 AF647. 对 MCF7 (A), A549 (B), SK-BR-3 (C) 和 HeLa (D) 全细胞裂解液及人睾丸组织提取物 (E) 中 G6PD 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker™ MW Tag-Alexa Fluor® 488 检测 Cruz Marker™ 分子量标准: sc-516790.

G6PD 抗体 (G-12): sc-373886

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寫評論提問

说明书
  • G6PD 抗体 G-12 是小鼠单克隆 IgG1 κ，G6PD抗体, 在40篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列356-515位于human物种的G6PD的C-terminus
  • G6PD 抗体 (G-12) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 G6PD
  • 抗G6PD抗体（G-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取G6PD (G-12): sc-373886的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是G6PD Antibody (G-12) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与G6PD Antibody (G-12) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

G6PD抗体（G-12）是一种IgG1κ小鼠单克隆G6PD抗体（也称为葡萄糖-6-磷酸1-脱氢酶抗体或G6PD1抗体），可通过WB、IP、IF、IHC（P）和ELISA检测人源的G6PD蛋白。G6PD抗体（G-12）可以以非偶联抗G6PD抗体形式和多种偶联抗G6PD抗体形式提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。葡萄糖-6-磷酸1-脱氢酶（G6PD）在磷酸戊糖途径中起着重要作用。它是葡萄糖-6-磷酸脱氢酶家族蛋白的成员。G6PD是一种普遍存在的酶，产生戊糖用于核酸合成，但也参与碳水化合物的降解，因为它是NADPH还原力的主要生产者之一。G6PD以NADP作为辅因子和结构元素。它可以以同源二聚体或同源四聚体的形式存在，主要在淋巴母细胞、粒细胞和精子中检测到。G6PD的缺陷可导致慢性非球形溶血性贫血（CNSHA），尤其是在疟疾流行的地区。G6PD缺陷水平较高的个体发生急性溶血发作的风险较高。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

G6PD 抗体 (G-12) 参考文献:

  1. 树胶糖和 6-氨基己酸可使因糖化和盐酸胍而失活的葡萄糖-6-磷酸脱氢酶稳定和复性。  |  Ganea, E. and Harding, JJ. 2005. Biol Chem. 386: 269-78. PMID: 15843172
  2. 慢性细小病毒感染和伪装成钻石-贝克范贫血症的 G6PD 缺乏症。  |  Parekh, S., et al. 2005. Am J Hematol. 79: 54-7. PMID: 15849759
  3. 葡萄糖-6-磷酸和 NADP+ 与人类葡萄糖-6-磷酸脱氢酶结合的结构研究。  |  Kotaka, M., et al. 2005. Acta Crystallogr D Biol Crystallogr. 61: 495-504. PMID: 15858258
  4. 与成人非结合性高胆红素血症有关的遗传因素。  |  Huang, CS., et al. 2005. Pharmacogenet Genomics. 15: 43-50. PMID: 15864125
  5. 甲状腺上皮细胞单层培养物中葡萄糖-6-磷酸脱氢酶的活性：促甲状腺激素和氧化氮合酶抑制剂会影响酶活性和组织化学分析的氧敏感性。  |  Kayser, L. and Thomsen, J. 2005. Acta Histochem. 107: 31-41. PMID: 15866284
  6. G6PD 缺乏症的诊断和管理。  |  Frank, JE. 2005. Am Fam Physician. 72: 1277-82. PMID: 16225031
  7. 编码人类 6-磷酸葡萄糖脱氢酶的 X 连锁基因的结构分析。  |  Martini, G., et al. 1986. EMBO J. 5: 1849-55. PMID: 2428611
  8. 葡萄糖 6 磷酸酶脱氢酶（G6PD）与神经退行性疾病：绘制诊断和治疗机会图。  |  Tiwari, M. 2017. Genes Dis. 4: 196-203. PMID: 30258923
  9. 人葡萄糖-6-磷酸脱氢酶（G6PD）cDNA 克隆的分离：蛋白质的一级结构和不寻常的 5' 非编码区。  |  Persico, MG., et al. 1986. Nucleic Acids Res. 14: 2511-22. PMID: 3515319
  10. 葡萄牙人口中葡萄糖-6-磷酸脱氢酶 (G6PD) 缺乏症的分子异质性。  |  Manco, L., et al. 2023. Acta Med Port. 36: 81-87. PMID: 36150187
  11. G6PD 活性与细胞凋亡信号之间的乙酰化依赖性耦合。  |  Wu, F., et al. 2023. Nat Commun. 14: 6208. PMID: 37798264
  12. 槲皮素抑制 G6PD 可促进表皮生长因子受体 T790M 突变的降解。  |  Ge, Z., et al. 2023. Cell Rep. 42: 113417. PMID: 37950872

订购信息

产品名称产品编号规格价格数量收藏夹

G6PD 抗体 (G-12)

sc-373886
200 µg/ml
$316.00

G6PD (G-12): m-IgG Fc BP-HRP 套装

sc-529597
200 µg Ab; 10 µg BP
$354.00

G6PD (G-12): m-IgGκ BP-HRP 套装

sc-522542
200 µg Ab, 40 µg BP
$354.00

G6PD (G-12): m-IgG1 BP-HRP 套装

sc-543599
200 µg Ab; 20 µg BP
$354.00

G6PD 抗体 (G-12) AC

sc-373886 AC
500 µg/ml, 25% agarose
$416.00

G6PD 抗体 (G-12) HRP

sc-373886 HRP
200 µg/ml
$316.00

G6PD 抗体 (G-12) FITC

sc-373886 FITC
200 µg/ml
$330.00

G6PD 抗体 (G-12) PE

sc-373886 PE
200 µg/ml
$343.00

G6PD 抗体 (G-12) Alexa Fluor® 488

sc-373886 AF488
200 µg/ml
$357.00

G6PD 抗体 (G-12) Alexa Fluor® 546

sc-373886 AF546
200 µg/ml
$357.00

G6PD 抗体 (G-12) Alexa Fluor® 594

sc-373886 AF594
200 µg/ml
$357.00

G6PD 抗体 (G-12) Alexa Fluor® 647

sc-373886 AF647
200 µg/ml
$357.00

G6PD 抗体 (G-12) Alexa Fluor® 680

sc-373886 AF680
200 µg/ml
$357.00

G6PD 抗体 (G-12) Alexa Fluor® 790

sc-373886 AF790
200 µg/ml
$357.00

Please help me that if i have the mouse anti-human G6PD Antibody (G-12) HRP (373886 HRP) for the secondary antibody of ELISA assay. Which primary antibody should i use for coating? Thank you in advance

Asked by: xtltam
Thank you for your question. The G6PD mouse monoclonal antibody you have, sc-373886 HRP, is a primary antibody already conjugated to HRP, so you do not need to use a secondary antibody. We do not offer paired antibodies for ELISA, but you should be able to use any other G6PD antibody as long as the epitope is different.
Answered by: Tech Service
Date published: 2020-01-03

I am using G6PD (G-12): sc-373886 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good antibodyWe used this antibody to assess G6PD levels in human non-small cell lung cancer cells by western blot. We got a good signal with 1:1000 dilution and below, that decreased in treated cells, indicating a high specificity.
Date published: 2022-01-21
Rated 5 out of 5 by from Well done!We used this antibody to assess G6PD silencing efficiency in human myeloid cells by western blot. We got a good signal with 1:1000 dilution and below, that decreased in silenced cells, indicating a high specificity.
Date published: 2021-05-26
Rated 1 out of 5 by from NOt working on mouse epithelial cellsAlong with sc-373887, this G6PD antibody could not detect G6PD protein band in mouse thymic epithelial cell while it reacted with human origin positive cell lines and antibody from different company could show.
Date published: 2018-02-01
Rated 5 out of 5 by from Great antibody for western blotThe batch of antibody I have worked well onHeLa, MCF7, Jurkat and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 500 times into 5% BSA, TBST and incubated over-night at 4°C.
Date published: 2018-01-04
Rated 5 out of 5 by from Great antibodyAntibody works well - clear, specific band for western blot with 25 ug U87 cell lysates at 1:1000 primary antibody dilution.
Date published: 2017-09-10
Rated 5 out of 5 by from GREAT for WBIt is good for wb,and it is 1:500 in 5% bsa,4℃ overnight。
Date published: 2017-08-09
Rated 5 out of 5 by from the antibody was very nice for WB and IFthe antibody gives a nice signal in the boar sperm
Date published: 2017-03-06
Rated 5 out of 5 by from GreatIt is a very clean antibody for western blotting. 1:200 dilution, works well.
Date published: 2017-02-07
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G6PD 抗体 (G-12) is rated 4.5 out of 5 by 11.
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