Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FXR1 CRISPR/Cas9 KO Plasmid (m): sc-420430
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FXR1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FXR1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FXR1 Antibody (B-2): sc-374148
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FXR1 CRISPR/Cas9 KO Plasmid (m) | sc-420430 | 20 µg | $397.00 |
Overview
Fxr1 encodes fragile X mental retardation syndrome-related protein 1 (FXR1), an RNA-binding protein that regulates post-transcriptional gene expression through control of mRNA stability, localization, and translation. FXR1 associates with ribonucleoprotein granules and participates in RNA metabolism programs that shape cell growth, differentiation, and stress responses, including modulation of cytoskeletal and muscle-related transcripts. In mouse, Fxr1 contributes to tissue-specific gene expression and developmental processes where tight translational control is required. Dysregulation of FXR1-dependent RNA networks has been linked in the literature to altered proliferation and survival signaling and to phenotypes relevant to neurodevelopmental and neuromuscular biology.
FXR1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Fxr1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Fxr1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Fxr1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FXR1 protein expression.
This CRISPR knockout system enables efficient generation of Fxr1-deficient cell models for investigation of FXR1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Fxr1 exon(s) critical for FXR1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Fxr1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FXR1 CRISPR/Cas9 KO Plasmid (m) and FXR1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Fxr1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FXR1 HDR Plasmid (m) and FXR1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Fxr1 homology arms to support homology-directed repair at defined Fxr1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.