Fra1 Antibody (B-10): sc-48424
- Fra1 Antibody (B-10) is a mouse monoclonal IgG1, cited in 2 publications, provided at 200 µg/ml
- recommended for detection of Fra-1 of mouse, rat and human origin by WB, IP, IF and ELISA
- See Fra1 (D-3): sc-376148 for Fra1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Fra1 Antibody (B-10). This work is in progress.
Fra-1 Antibody (B-10) is a mouse monoclonal IgG1 antibody raised against human Fra-1, a critical member of the activator protein-1 (AP-1) transcription factor family. Anti-Fra-1 antibody (B-10) demonstrates broad species cross-reactivity, detecting Fra-1 in human, mouse, and rat samples through Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) applications. As a nuclear phosphoprotein encoded by the FOSL1 gene, Fra-1 operates through a sophisticated mechanism involving its basic leucine zipper (bZIP) domain. This domain enables Fra-1 to form heterodimers with Jun family proteins, creating complexes that bind specific DNA elements to regulate gene expression. Fra-1′s significance becomes particularly evident in cancer biology, where dysregulation of Fra-1 contributes to cancer progression, notably in aggressive forms like triple-negative breast cancer (TNBC). Beyond cancer, Fra-1 serves as a crucial regulator of immune system function, influencing immune cell states and contributing to inflammatory conditions such as arthritis, psoriasis, and cardiovascular diseases. Fra-1′s effects show remarkable context-dependence, varying based on cell type, environmental conditions, and specific stimuli, making Fra-1 an important therapeutic target across various diseases. Through these diverse cellular functions, Fra-1 (B-10) antibody provides researchers with a valuable tool for investigating both normal physiology and disease mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Fra1 Antibody (B-10) References:
- Fos family members: regulation, structure and role in oncogenic transformation. | Tulchinsky, E. 2000. Histol Histopathol. 15: 921-8. PMID: 10963134
- Fra-1 replaces c-Fos-dependent functions in mice. | Fleischmann, A., et al. 2000. Genes Dev. 14: 2695-700. PMID: 11069886
- Transactivation of Fra-1 and consequent activation of AP-1 occur extracellular signal-regulated kinase dependently. | Young, MR., et al. 2002. Mol Cell Biol. 22: 587-98. PMID: 11756554
- The duration of ERK1/2 activity determines the activation of c-Fos and Fra-1 and the composition and quantitative transcriptional output of AP-1. | Chalmers, CJ., et al. 2007. Cell Signal. 19: 695-704. PMID: 17052890
- Ubiquitin-independent proteasomal degradation of Fra-1 is antagonized by Erk1/2 pathway-mediated phosphorylation of a unique C-terminal destabilizer. | Basbous, J., et al. 2007. Mol Cell Biol. 27: 3936-50. PMID: 17371847
- FIAT, the factor-inhibiting ATF4-mediated transcription, also represses the transcriptional activity of the bZIP factor FRA-1. | St-Arnaud, R., et al. 2010. Ann N Y Acad Sci. 1192: 338-43. PMID: 20392257
- Old players with a newly defined function: Fra-1 and c-Fos support growth of human malignant breast tumors by activating membrane biogenesis at the cytoplasm. | Motrich, RD., et al. 2013. PLoS One. 8: e53211. PMID: 23301044
- FRA-1 as a driver of tumour heterogeneity: a nexus between oncogenes and embryonic signalling pathways in cancer. | Dhillon, AS. and Tulchinsky, E. 2015. Oncogene. 34: 4421-8. PMID: 25381818
- Fra-1 induces apoptosis and neuroinflammation by targeting S100A8 to modulate TLR4 pathways in spinal cord ischemia/reperfusion injury. | Chen, Y., et al. 2023. Brain Pathol. 33: e13113. PMID: 36634215
- Fra-1, a Fos gene family member that activates atrial natriuretic peptide gene transcription. | Kovacic-Milivojevic, B. and Gardner, DG. 1995. Hypertension. 25: 679-82. PMID: 7721415
- A C-terminal domain in FosB, absent in FosB/SF and Fra-1, which is able to interact with the TATA binding protein, is required for altered cell growth. | Metz, R., et al. 1994. EMBO J. 13: 3832-42. PMID: 8070410
- Structure and chromosomal assignment of the mouse fra-1 gene, and its exclusion as a candidate gene for oc (osteosclerosis). | Schreiber, M., et al. 1997. Oncogene. 15: 1171-8. PMID: 9294610
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Fra1 Antibody (B-10) | sc-48424 | 200 µg/ml | $322.00 |