Date published: 2025-9-6
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FOXM1 Antibody (G-5) - Western Blotting - Image 353178
FOXM1 抗体 (G-5): sc-376471. PC-12 (A), NIH/3T3 (B), U-2 OS (C), Caco-2 (D), F9 (E) 和 MCF7 (F) 全细胞裂解液中 FOXM1 表达的 Western 印迹分析.
FOXM1 抗体 (G-5): sc-376471. PC-12 (A), NIH/3T3 (B), U-2 OS (C), Caco-2 (D), F9 (E) 和 MCF7 (F) 全细胞裂解液中 FOXM1 表达的 Western 印迹分析.

FOXM1 抗体 (G-5): sc-376471

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说明书
  • FOXM1 抗体 G-5 是小鼠单克隆 IgG2b κ，FOXM1抗体, 在64篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的FOXM1的C-terminus的氨基酸734-763之间
  • FOXM1 抗体 (G-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 all isoforms of FOXM1
  • 抗FOXM1抗体（G-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-376471 X, 200 µg/0.1 ml)
  • 关于如何获取FOXM1 (G-5): sc-376471的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 FOXM1 抗体 (G-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与FOXM1 抗体 (G-5) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外FOX抗体，包括FOXC1, FOXC2, FOXD1, FOXD3, FOXE3, FOXF2, FOXJ1, FOXJ2, FOXK1, FOXL1, FOXL2, FOXM1, FOXN1, FOXN2, FOXN4和FOXP1

FOXM1抗体（G-5）是一种IgG2b κ小鼠单克隆FOXM1抗体（也被称为HNF3抗体、FKHL16抗体、forkhead box M1抗体），可通过WB、IP、IF、IHC(P)和ELISA方法检测小鼠、大鼠和人类来源的FOXM1蛋白。FOXM1抗体（G-5）既有非偶联形式的抗FOXM1抗体，也有多种偶联形式的抗FOXM1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Fox转录因子家族是一大类蛋白质，它们共享一个称为翼状螺旋或叉头域的常见DNA结合域。FOXM1，也被称为FKHL16、MPP2或Trident，主要在增殖细胞中表达。编码人类FOXM1的基因映射到12p13染色体上。将FOXM1表达限制在增殖细胞中的转录元件位于起始密码子上游300bp处。FOXM1在胸腺、睾丸、小肠和结肠中含量最为丰富。选择性剪接产生含有PEST区域的FOXM1A和FOXM1B异构体，这些区域参与快速蛋白质降解。FOXM1表达的减少与细胞增殖的年龄相关缺陷有关。相反，在老年转基因小鼠的肝脏中增加FOXM1B表达，可以将肝细胞DNA复制率恢复到年轻肝脏中的较高水平。FOXM1B激活细胞周期蛋白B1、细胞周期蛋白D1和Cdc25B的转录。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

FOXM1 抗体 (G-5)

sc-376471
200 µg/ml
$316.00

FOXM1 (G-5): m-IgG Fc BP-HRP 套装

sc-529873
200 µg Ab; 10 µg BP
$354.00

FOXM1 (G-5): m-IgGκ BP-HRP 套装

sc-522963
200 µg Ab, 40 µg BP
$354.00

FOXM1 (G-5): m-IgG2b BP-HRP 套装

sc-550146
200 µg Ab; 10 µg BP
$354.00

FOXM1 抗体 (G-5) X

sc-376471 X
200 µg/0.1 ml
$316.00

FOXM1 抗体 (G-5) AC

sc-376471 AC
500 µg/ml, 25% agarose
$416.00

FOXM1 抗体 (G-5) HRP

sc-376471 HRP
200 µg/ml
$316.00

FOXM1 抗体 (G-5) FITC

sc-376471 FITC
200 µg/ml
$330.00

FOXM1 抗体 (G-5) PE

sc-376471 PE
200 µg/ml
$343.00

FOXM1 抗体 (G-5) Alexa Fluor® 488

sc-376471 AF488
200 µg/ml
$357.00

FOXM1 抗体 (G-5) Alexa Fluor® 546

sc-376471 AF546
200 µg/ml
$357.00

FOXM1 抗体 (G-5) Alexa Fluor® 594

sc-376471 AF594
200 µg/ml
$357.00

FOXM1 抗体 (G-5) Alexa Fluor® 647

sc-376471 AF647
200 µg/ml
$357.00

FOXM1 抗体 (G-5) Alexa Fluor® 680

sc-376471 AF680
200 µg/ml
$357.00

FOXM1 抗体 (G-5) Alexa Fluor® 790

sc-376471 AF790
200 µg/ml
$357.00

FOXM1 (G-5) 中和勝肽

sc-376471 P
100 µg/0.5 ml
$68.00

FOXM1 G-5 is mouse monoclonal IgG2b. I would like to know if it can be used with conventional secondary anti-mouse IgG reagents.

Asked by: Gemo
Thank you for your question. Yes, you can use a conventional anti-mouse IgG secondary antibody for detection of this primary antibody. For more specific results, we do recommend using an isotype-specific secondary antibody (anti-mouse IgG2b), or our mouse IgGκ binding protein, e.g., m-IgGκ BP-HRP: sc-516102.
Answered by: Technical Support
Date published: 2020-03-09

Cantidad de anticuerpo FOXM1(G5) X TransCruz sc-376471 recomendado para usar en ChIP.

Asked by: femili12
Gracias por sus preguntas. Recomendamos el uso de 100-500 μg de sobrenadante y 0,1-1 μl de reactivo TransCruz (0,2-2 μg). Para obtener más información, haga clic en este enlace de nuestro protocolo ChIP: https://www.scbt.com/scbt/resources/protocols/chromatin-immunoprecipitation-assays Thank you for your questions. We recommend using 100–500 µg supernatant and 0.1-1 µl TransCruz reagent (0.2–2 µg). For further information, please click on this link to our ChIP protocol: https://www.scbt.com/scbt/resources/protocols/chromatin-immunoprecipitation-assays
Answered by: Technical Support
Date published: 2018-12-31

Which type of FOXM1  give good results for IHC on parrafin bock,human tissue breast cancer

Asked by: Madyan
Thank you for your question. We would recommend using our mouse monoclonal antibody, FOXM1 (G-5): sc-376471. We have several pieces of IHC(P) data in human tissue using this antibody on our website.
Answered by: Technical Support
Date published: 2017-08-22

Does the FOXM1 Antibody (G-5): sc-376471 detect isoform “C” of the FOXM1 target?

Asked by: Trav11
Thank you for your question. The FOXM1 Antibody (G-5): sc-376471 will detect all isoforms (A, B, & C) for the FOXM1 protein target. If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 15
Date published: 2017-01-16

Does FOXM1 Antibody (G-5): sc-376471 react with Zebrafish samples?

Asked by: Trav11
Thank you for your question. For additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or by using the Live Chat function on our website and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Technical Support 13
Date published: 2017-01-13
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Rated 5 out of 5 by from 功能不错，敏感度很高，很好跑半年前试用了一周，感觉效果很棒，再次购买发现没让人失望，效果真的很不错，以后还会再买他家的抗体，都可以试试。
Date published: 2023-10-11
Rated 4 out of 5 by from 经理BACKGROUND The Fox family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. FOXM1, also known as FKHL16, MPP2 or Trident, is primarily expressed in proliferating cells. The gene encoding human FOXM1 maps to chromosome 12p13.33. The transcription element that restricts FOXM1 expression to prolif- erating cells is located 300 bp upstream of the start codon. FOXM1 is most abundant in thymus, testis, small intestine and colon. Alternative splicing generates FOXM1A and FOXM1B isoforms that contain PEST regions involved in rapid protein degradation. A decrease in FOXM1 expression is associated with age-related defects in cellular proliferation. Conversely, an increase in FOXM1B expression in the livers of older transgenic mice restore hepatocyte DNA replication rates to the higher rate present in young livers. FOXM1B activates the transcription of cyclin B1, cyclin D1 and Cdc25B
Date published: 2021-06-23
Rated 5 out of 5 by from the greatest Ab i ever had!it works very well in both KD and OE systems i set up. believe me, commercial ABs of FOXM1 from other companies dont work at all. this showed clear band, at 1:250 dilution with senescent dermal fibroblast cell samples.
Date published: 2020-07-22
Rated 1 out of 5 by from Not specific for FOXM1 - Do not use this antibody!As noticed by other users before, the FOXM1 G-5 antibody detects a protein with the same molecular weight as FOXM1 in Western Blots, but
Date published: 2018-07-18
Rated 4 out of 5 by from Great antibodiesI got a free sample from company. I used antibodies in WB in dilution 1:1000. It works good.
Date published: 2018-07-14
Rated 1 out of 5 by from Not sure if observed band is FOXM1Like the previous comments below, our group has also confirmed that this antibody may not be recognizing FOXM1. We observe a strong clear band at around 125-130 kDa with this antibody in our cell lines. However, upon using both siRNA and shRNA approaches with 3 different species, we could not observe a clear knockdown of FOXM1 with this antibody. In contrast, FOXM1 (A-11) sc-271746 showed knockdown of FOXM1 protein. Additionally, while this antibody can recognize overexpressed FOXM1, the exogenous FOXM1 appears as a second at around 110 kDa. The FOXM1 (A-11) antibody only recognizes a single band with much greater intensity. The FOXM1 (A-11) antibody also gave consistent results with CS #D12D5 and an Abcam antibody.
Date published: 2018-05-11
Rated 3 out of 5 by from Gives good band, but uncertain about specificityThe mouse G-5 antibody appears to detect primarily nuclear FOXM1; whereas the SC-500 rabbit antibody detects mainly cytoplasmic (and to some extent nuclear). The expression pattern in western blots, ICC, and IHC have confirmed this (images attached). Possibly due to the G-5 antibody unable to detect FOXM1 in cytoplasm if dimerized? It seems to detect FOXM1 differently in regards to localization; however, the MW appears to be correct. I still haven't quite figured this one out. In any case, I used the antibody at a 1:1000 dilution and secondary at 1:2500. I've noticed most SC antibodies work well at 1:250/500 primary and secondary at 1:2500. It has worked well with overexpression (human FOXM1b isoform) as well as siRNA knockdown in rat cells.
Date published: 2018-03-26
Rated 1 out of 5 by from Not sure the band we detect is FOXM1We have observed similar results than the one showed by elenaenzo with two different siRNA against FOXM1 in HeLa cells (an antibody against FOXM1 from another firm was used as a positive control on the same lysates).
Date published: 2018-03-16
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FOXM1 抗体 (G-5) is rated 4.0 out of 5 by 28.
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