Date published: 2025-9-6

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FOXC2 抗体 (G-7): sc-515234

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说明书
  • FOXC2 抗体 G-7 是小鼠单克隆 IgG2b κ,FOXC2抗体, 在8篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列31-70位于human物种的FOXC2的N-terminus附近
  • FOXC2 抗体 (G-7) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 FOXC2
  • 抗FOXC2抗体(G-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取FOXC2 (G-7): sc-515234免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRPm-IgGκ BP-HRP是FOXC2 Antibody (G-7) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与FOXC2 Antibody (G-7) 打包提供(请参阅下面的订购信息)。

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関連項目

FOXC2抗体(G-7)是一种小鼠单克隆IgG2b kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类的FOXC2蛋白。FOXC2 单克隆抗体(G-7)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种 Alexa Fluor® 结合物。FOXC2是叉头/翼螺旋转录因子家族的一员,通过改变脂肪细胞PKA全酶的组成,提高β-肾上腺素能-cAMP-蛋白激酶A(PKA)信号通路的敏感性,从而在调节脂肪细胞代谢中发挥关键作用。这一功能尤为重要,因为高脂肪饮食通常会导致FOXC2水平升高,从而缓解许多肥胖相关症状,这凸显了FOXC2作为代谢紊乱治疗靶标的潜力。此外,FOXC2在体内和体外均与软骨分化早期阶段有关,而FOXC2单基因不足与淋巴水肿-双排睫毛(LD)有关,这是一种常染色体显性遗传病,其特征是肢体淋巴水肿和双排睫毛。此外,研究表明,FOXC2对于骨骼组织发育至关重要,缺乏FOXC2的突变小鼠的轴向和颅骨骨骼发生缺陷证明了这一点。值得注意的是,FOXC2与FOXC1在Notch信号通路中相互作用,该通路对于肾脏和心脏发育至关重要,这强调了FOXC2在代谢调节和发育过程中的多方面作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

FOXC2 抗体 (G-7) 参考文献:

  1. 小鼠叉头/翼螺旋基因Foxc1(Mf1)和Foxc2(Mfh1)是肾脏和泌尿道早期器官形成所必需的。  |  Kume, T., et al. 2000. Development. 127: 1387-95. PMID: 10704385
  2. 叉头家族转录因子 FOXC2(MFH-1)的突变是遗传性淋巴水肿-肢体畸形综合征的罪魁祸首。  |  Fang, J., et al. 2000. Am J Hum Genet. 67: 1382-8. PMID: 11078474
  3. FOXC2 是一种翼螺旋基因,可对抗肥胖, 高甘油三酯血症和饮食引起的胰岛素抵抗。  |  Cederberg, A., et al. 2001. Cell. 106: 563-73. PMID: 11551504
  4. 小鼠翼螺旋转录因子 Foxc1 和 Foxc2 都是心血管发育和体细胞发生所必需的。  |  Kume, T., et al. 2001. Genes Dev. 15: 2470-82. PMID: 11562355
  5. 小鼠中胚层细胞系 C1 和骨骼前体细胞中叉头/翼螺旋转录因子 Foxc2 (Mfh1) 的骨形态发生蛋白调控。  |  Nifuji, A., et al. 2001. J Bone Miner Res. 16: 1765-71. PMID: 11585339

订购信息

产品名称产品编号规格价格数量收藏夹

FOXC2 抗体 (G-7)

sc-515234
200 µg/ml
$316.00

FOXC2 (G-7): m-IgGκ BP-HRP 套装

sc-524983
200 µg Ab, 40 µg BP
$354.00

FOXC2 (G-7): m-IgG2b BP-HRP 套装

sc-549203
200 µg Ab; 10 µg BP
$354.00

FOXC2 抗体 (G-7) AC

sc-515234 AC
500 µg/ml, 25% agarose
$416.00

FOXC2 抗体 (G-7) HRP

sc-515234 HRP
200 µg/ml
$316.00

FOXC2 抗体 (G-7) FITC

sc-515234 FITC
200 µg/ml
$330.00

FOXC2 抗体 (G-7) PE

sc-515234 PE
200 µg/ml
$343.00

FOXC2 抗体 (G-7) Alexa Fluor® 488

sc-515234 AF488
200 µg/ml
$357.00

FOXC2 抗体 (G-7) Alexa Fluor® 546

sc-515234 AF546
200 µg/ml
$357.00

FOXC2 抗体 (G-7) Alexa Fluor® 594

sc-515234 AF594
200 µg/ml
$357.00

FOXC2 抗体 (G-7) Alexa Fluor® 647

sc-515234 AF647
200 µg/ml
$357.00

FOXC2 抗体 (G-7) Alexa Fluor® 680

sc-515234 AF680
200 µg/ml
$357.00

FOXC2 抗体 (G-7) Alexa Fluor® 790

sc-515234 AF790
200 µg/ml
$357.00

I was wondering if this antibody has been tested for Immunohistochemistry. I have grown two tumor xenografts group, one as a control and the other one is a FOXC2 Knock-down cell line. I want to stain for FOXC2 on FFPE blocks.

Asked by: JoelRT
Thank you for your question. Anti-FOXC2 Antibody (G-7) is only recommended for detection of FOXC2 of mouse, rat and human origin by WB, IP, IF and ELISA. We do not recommend it for IHC(P) applications based on our tests with this antibody.
Answered by: Technical Support Europe
Date published: 2020-10-23

What is the recommended starting dilution for Western blot for sc-515234: FOXC2 (G-7) monoclonal antibody?

Asked by: jerojero
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Good for Western blotAntibody works for Western blot with human dermal lymphatic endothelial cell (hdLECs) lysate in published research. -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from Nice stainingNice staining in ICC using mouse lymphatic endothelial cells
Date published: 2022-09-01
Rated 4 out of 5 by from Good productIt is good antibodies in WB. It works in dilytion 1:1000
Date published: 2018-07-14
Rated 3 out of 5 by from FOXC2 antibody EvaluationFOXC2 antibody was used in WB with 1:1000 concentration, this is the result:
Date published: 2017-01-31
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FOXC2 抗体 (G-7) is rated 4.3 out of 5 by 4.
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