FMO1 Double Nickase Plasmid (h): sc-404620-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FMO1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- FMO1 Double Nickase Plasmid (h) and FMO1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting FMO1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FMO1 Antibody (H-10): sc-376924
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FMO1 Double Nickase Plasmid (h) | sc-404620-NIC | 20 µg | $410.00 | |||
FMO1 Double Nickase Plasmid (h2) | sc-404620-NIC-2 | 20 µg | $410.00 |
Flavin-containing monooxygenase 1 (FMO1) is a microsomal NADPH-dependent monooxygenase that catalyzes oxygenation of diverse nucleophilic substrates, including xenobiotics and endogenous amines, as part of phase I metabolic clearance. In human tissues, FMO1 contributes to redox and detoxification processes within the endoplasmic reticulum and interfaces functionally with broader drug-metabolism networks alongside cytochrome P450 enzymes. Variation in FMO1 expression or activity can influence biotransformation capacity, affecting susceptibility to chemical stress and shaping exposure-dependent cellular phenotypes. FMO1 is also relevant to mechanistic studies of metabolic perturbation, oxidative byproduct handling, and inter-individual differences in xenobiotic response.
FMO1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the FMO1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within FMO1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt FMO1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of FMO1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.