Date published: 2025-9-7
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Flt-4 Antibody (E-3) - Western Blotting - Image 333365
Flt-4 抗体 (E-3): sc-514825. HEL 92. 1. 7 全细胞裂解液中 Flt-4 表达的 Western 印迹分析.
Flt-4 Antibody (E-3) - Western Blotting - Image 333365
Flt-4 抗体 (E-3): sc-514825. HEL 92. 1. 7 全细胞裂解液中 Flt-4 表达的 Western 印迹分析.
Flt-4 Antibody (E-3) - Western Blotting - Image 386653
用 Flt-4 抗体 (E-3) Alexa Fluor® 790: sc-514825 AF790 检测 HEL 92. 1. 7 全细胞裂解液中 Flt-4 的表达, 用β-Actin (C4) Alexa Fluor® 680: sc-47778 AF680 检测β-Actin的表达. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
Flt-4 Antibody (E-3) - Western Blotting - Image 375121
Flt-4 抗体 (E-3): sc-514825. TF-1 全细胞裂解液中 Flt-4 表达的 Western 印迹分析.
Flt-4 抗体 (E-3): sc-514825. HEL 92. 1. 7 全细胞裂解液中 Flt-4 表达的 Western 印迹分析.

Flt-4 抗体 (E-3): sc-514825

2.3(3)
寫評論提問

说明书
  • Flt-4 抗体 E-3 是小鼠单克隆 IgG1 κ，Flt-4抗体, 在5篇文献中引用，规格为200 µg/ml
  • 特定于mouse起源的Flt-4的C-terminus内的氨基酸1278-1299之间的表位图谱
  • Flt-4 抗体 (E-3) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Flt-4
  • 抗Flt-4抗体（E-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Flt-4 (E-3): sc-514825的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和m-IgGκ BP-HRP是Flt-4 Antibody (E-3) 适用于 WB 应用。 的首选辅助检测试剂，这些试剂现与Flt-4 Antibody (E-3) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外VEGFR / Flt抗体，包括Flt-4

Flt-4抗体（E-3）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人源Flt-4蛋白。抗 -Flt-4抗体（E-3）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Flt-4，也称为血管内皮生长因子受体3（VEGFR-3），在淋巴管生成和淋巴系统发育中发挥着关键作用，而淋巴管生成和淋巴系统发育对于维持体液平衡和免疫反应至关重要。Flt-4主要位于内皮细胞表面，特别是在淋巴管中，Flt-4可调节血管内皮生长因子（VEGF）的作用，并调节淋巴内皮细胞的增殖和迁移。Flt-4的正常功能对于新淋巴管形成和淋巴管完整性维护至关重要，因此Flt-4成为癌症转移和炎症性疾病研究的重要目标。Flt-4与VEGF-C和VEGF-D相互作用，而VEGF-C和VEGF-D是激活受体的重要配体，这进一步凸显了Flt-4在血管生物学和潜在治疗应用中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Flt-4 抗体 (E-3) 参考文献:

  1. 人类结肠粘膜肿瘤进展过程中的 Vegf, Vegf-B, Vegf-C 及其受体 KDR, FLT-1 和 FLT-4。  |  André, T., et al. 2000. Int J Cancer. 86: 174-81. PMID: 10738243
  2. FLT4 受体酪氨酸激酶包含七个免疫球蛋白样环，在多种人体组织和细胞系中表达。  |  Pajusola, K., et al. 1992. Cancer Res. 52: 5738-43. PMID: 1327515
  3. 非小细胞肺癌中 Flt-4 阳性内皮细胞密度及其临床意义  |  Chen, F., et al. 2004. Clin Cancer Res. 10: 8548-53. PMID: 15623638
  4. 过渡性膀胱癌中的 VEGF-C、VEGF-D 和 Flt-4：与临床病理参数和长期生存的关系。  |  Herrmann, E., et al. 2007. Anticancer Res. 27: 3127-33. PMID: 17970053
  5. Fms样酪氨酸激酶（Flt）-4信号参与骨质破坏（op/op）小鼠的破骨细胞分化。  |  Tsuka, N., et al. 2009. Biomed Res. 30: 31-7. PMID: 19265261
  6. 染料木素可抑制 FLT4 并抑制人类结直肠癌的转移。  |  Xiao, X., et al. 2015. Oncotarget. 6: 3225-39. PMID: 25605009
  7. 淋巴丝虫病中的 FOXC2 和 FLT4 基因变异。  |  Sheik, Y., et al. 2015. Lymphat Res Biol. 13: 112-9. PMID: 26091406
  8. FLT4靶向纳米载体的表面工程增强了细胞软化青光眼疗法。  |  Vincent, MP., et al. 2021. ACS Appl Mater Interfaces. 13: 32823-32836. PMID: 34232612
  9. 与两种血管内皮生长因子受体有关的蛋白水解受体酪氨酸激酶 FLT4 的信号特性。  |  Pajusola, K., et al. 1994. Oncogene. 9: 3545-55. PMID: 7970715
  10. 急性髓性白血病中 FLT4 及其配体 VEGF-C 的表达。  |  Fielder, W., et al. 1997. Leukemia. 11: 1234-7. PMID: 9264375

订购信息

产品名称产品编号规格价格数量收藏夹

Flt-4 抗体 (E-3)

sc-514825
200 µg/ml
$316.00

Flt-4 (E-3): m-IgG Fc BP-HRP 套装

sc-531227
200 µg Ab; 10 µg BP
$354.00

Flt-4 (E-3): m-IgGκ BP-HRP 套装

sc-524854
200 µg Ab, 40 µg BP
$354.00

Flt-4 抗体 (E-3) AC

sc-514825 AC
500 µg/ml, 25% agarose
$416.00

Flt-4 抗体 (E-3) HRP

sc-514825 HRP
200 µg/ml
$316.00

Flt-4 抗体 (E-3) FITC

sc-514825 FITC
200 µg/ml
$330.00

Flt-4 抗体 (E-3) PE

sc-514825 PE
200 µg/ml
$343.00

Flt-4 抗体 (E-3) Alexa Fluor® 488

sc-514825 AF488
200 µg/ml
$357.00

Flt-4 抗体 (E-3) Alexa Fluor® 546

sc-514825 AF546
200 µg/ml
$357.00

Flt-4 抗体 (E-3) Alexa Fluor® 594

sc-514825 AF594
200 µg/ml
$357.00

Flt-4 抗体 (E-3) Alexa Fluor® 647

sc-514825 AF647
200 µg/ml
$357.00

Flt-4 抗体 (E-3) Alexa Fluor® 680

sc-514825 AF680
200 µg/ml
$357.00

Flt-4 抗体 (E-3) Alexa Fluor® 790

sc-514825 AF790
200 µg/ml
$357.00

Flt-4 (E-3) 中和勝肽

sc-514825 P
100 µg/0.5 ml
$68.00

Dear Santa Cruz, I need an antibody to detect VEGFR3 in canine tissue. I found sc-321 used in dogs, but it's rveplaced with E-3. Does E-3 cross-react with canine VEGFR3? If not, can sc-321 be custom ordered? May I ask your email to share more info?

Asked by: kang
Thank you for your question. sc-514825 Flt-4 (E-3) has not been validated for cross reactivity for canine. The antibody is recommended for mouse,rat and human samples. If you have additional questions please contact technical service by emailing scbt@scbt.com.
Answered by: BlakeJ
Date published: 2025-04-18

Dear Santa Cruz, I need an antibody to detect VEGFR3 in canine tissue. I found sc-321 used in dogs, but it's replaced with E-3. Does E-3 cross-react with canine VEGFR3? If not, can sc-321 be custom ordered? May I ask your email to share more info?

Asked by: kang
Thank you for your question. Flt-4 Antibody (E-3) is recommended for detection of Flt-4 of mouse, rat and human origin by WB, IP, IF and ELISA. The product SC-321 has been discontinued and is no longer available for ordering or custom production.
Answered by: Ling Chen
Date published: 2025-04-17

What application is the blocking peptide sc-514825 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Not valid for IF on rat frozen liver sectionsRat liver frozen sections were subjected to immunofluorescence using Flt-4 (E-3) antibody. Antigen retrieval methods were optimized between without antigen retrieval, heat-induced citrate buffer, and trypsin-induced antigen retrieval. However, a positive signal was not clear on each slide.
Date published: 2018-03-01
Rated 4 out of 5 by from It works well.I purchased this antibody last month to perform an Immunofluorescence in zebrafish embryos and it was efficient.
Date published: 2017-12-13
Rated 2 out of 5 by from no specific staining was detected in human testiswe use e-3 for IHC in human testis at 1:100 dilution but no specific staining was detected
Date published: 2017-06-01
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Flt-4 抗体 (E-3) is rated 2.3 out of 5 by 3.
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