Date published: 2025-9-7
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Fli-1 Antibody (F-12) - Western Blotting - Image 354033
Fli-1 抗体 (F-12): sc-365294. BJAB (A), HEL 92. 1. 7 (B) 和 Jurkat (C) 全细胞裂解液中 Fli-1 表达的 Western 印迹分析.
Fli-1 抗体 (F-12): sc-365294. BJAB (A), HEL 92. 1. 7 (B) 和 Jurkat (C) 全细胞裂解液中 Fli-1 表达的 Western 印迹分析.

Fli-1 抗体 (F-12): sc-365294

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说明书
  • Fli-1 抗体 F-12 是小鼠单克隆 IgM κ，Fli-1抗体, 在4篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于mouse物种的Fli-1的C-terminus的氨基酸427-452之间
  • 推荐用于 mouse, rat 和 human 来源的Fli-1 WB, IP, IF 和 ELISA检测
  • 可用于ChIP的TransCruz试剂(sc-365294 X, 200 µg/0.1 ml)
  • 关于如何获取Fli-1 (F-12): sc-365294的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Fli-1 抗体 (F-12)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Fli 抗体包括 Fli-1

Fli-1抗体（F-12）是一种IgM κ小鼠单克隆Fli-1抗体（也称为FLI1抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的Fli-1蛋白。Fli-1抗体（F-12）为非偶联形式的抗Fli-1抗体。Ets-1是基于与从E26成红细胞增多症病毒中分离出的v-Ets癌基因的同源性而鉴定出的一系列基因的原型成员。目前，该基因家族包括Ets-1、Ets-2、Erg-1、Erg-2、Elk、E74、Fli-1、PU.1和PEA3。Ets基因家族的成员显示出不同的组织表达模式，并共享一个高度保守的羧基末端结构域，该结构域包含与SV40大T抗原核定位信号序列相关的序列。这个保守结构域对于Ets-1与DNA的结合至关重要，并且可能是Ets基因家族所有成员DNA结合活性的原因。其中一些蛋白质已被证明可以识别DNA中的相似基序，这些基序共享一个位于中心的5′-GGAA-3′元件。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Fli-1 抗体 (F-12) 参考文献:

  1. PEA3的分子克隆和特征描述，PEA3是Ets癌基因家族的一个新成员，在小鼠胚胎细胞中有不同表达。  |  Xin, JH., et al. 1992. Genes Dev. 6: 481-96. PMID: 1547944
  2. 尤文氏肉瘤融合蛋白, EWS/Fli-1 和 Fli-1 蛋白通过与 5' 启动子的 ETS 结构域结合，诱导 PLD2 而非 PLD1 基因表达。  |  Kikuchi, R., et al. 2007. Oncogene. 26: 1802-10. PMID: 16964281
  3. Caveolin-1（CAV1）是 EWS/FLI-1 的靶标，也是决定尤文氏肉瘤细胞致癌表型和致瘤性的关键因素。  |  Tirado, OM., et al. 2006. Cancer Res. 66: 9937-47. PMID: 17047056
  4. 果蝇 74EF 早期浮肿包含 E74，这是一个复杂的蜕皮激素诱导基因，编码两种与蜕皮激素相关的蛋白质。  |  Burtis, KC., et al. 1990. Cell. 61: 85-99. PMID: 2107982
  5. 麋鹿，X 和 14 号染色体上与组织特异性等位基因相关的基因，靠近易位断点。  |  Rao, VN., et al. 1989. Science. 244: 66-70. PMID: 2539641
  6. 红白血病细胞中Fli-1的过度表达会促进红细胞分化，而Spi-1/PU.1则具有相反的作用。  |  Vecchiarelli-Federico, LM., et al. 2017. Int J Oncol. 51: 456-466. PMID: 28586009
  7. Fli-1在鼠脓毒症模型中控制周细胞功能障碍。  |  Li, P., et al. 2018. J Infect Dis. 218: 1995-2005. PMID: 30053030
  8. c-ets 癌基因编码的 54,000 细胞质蛋白在胸腺和法氏囊淋巴细胞中的鉴定和优先表达。  |  Ghysdael, J., et al. 1986. Proc Natl Acad Sci U S A. 83: 1714-8. PMID: 3006066
  9. 21 号染色体上的人类 ets 相关基因 erg：替代剪接, 多腺苷酸化和翻译。  |  Rao, VN., et al. 1987. Science. 237: 635-9. PMID: 3299708
  10. 确定Mir-182-3p/FLI-1轴作为宫颈癌免疫反应中的关键信号： A Comprehensive Bioinformatic Analysis.  |  Salmerón-Bárcenas, EG., et al. 2023. Int J Mol Sci. 24: PMID: 37047006
  11. Ets1 转录因子在小鼠胚胎发育过程中广泛表达，与参与器官形成等形态发生过程的中胚层细胞有关。  |  Kola, I., et al. 1993. Proc Natl Acad Sci U S A. 90: 7588-92. PMID: 7689222
  12. PU.1 磷酸化对与 NF-EM5 的相互作用和转录激活的影响。  |  Pongubala, JM., et al. 1993. Science. 259: 1622-5. PMID: 8456286

订购信息

产品名称产品编号规格价格数量收藏夹

Fli-1 抗体 (F-12)

sc-365294
200 µg/ml
$316.00

Fli-1 (F-12) 中和勝肽

sc-365294 P
100 µg/0.5 ml
$68.00

Fli-1 抗体 (F-12) X

sc-365294 X
200 µg/0.1 ml
$316.00

What application is the blocking peptide sc-365294 P appropriate for?

Asked by: Trav11
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Does not detect EWS-FLI1 FusionI bought this antibody to detect the fusion protein of Ewing sarcoma. It is supposed to detect the C-terminus of FLI1, but it was unable to detect the fusion protein in any cell lines.
Date published: 2020-01-03
Rated 1 out of 5 by from Did not detect Fli1-Ews fusionGot the trial size to detect the fli1-ews fusion protein in Ewing sarcoma. No signal detected
Date published: 2017-12-21
Rated 5 out of 5 by from good resultworking with this antibody.Great band obtained in WB on human liver tissue samples.
Date published: 2017-04-08
Rated 5 out of 5 by from Strong and ClearMethods The cellular localization of Fli-1 antibody in mouse kidney tissue was detected by immunoblot and immunohistochemistry. The analysis results of Western blot were very strong and clear.
Date published: 2017-02-08
Rated 5 out of 5 by from Good for WBThis antibody works well in Western Blot with a variety of cell lysates including BJAB, HEL 92.1.7 and Jurkat.
Date published: 2017-01-27
Rated 5 out of 5 by from worked goodUsed on Western Blot with mouse samples,recommended 1:500 dilution,worked good.
Date published: 2016-12-18
Rated 5 out of 5 by from Produced superb immunofluorescence nuclearProduced superb immunofluorescence nuclear and cytoplasmic staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-02-09
Rated 5 out of 5 by from Produced positive Western Blot data of FliProduced positive Western Blot data of Fli-1 expression in U-937 and RAW 264.7 whole cell lysates. -SCBT QC
Date published: 2013-02-10
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Fli-1 抗体 (F-12) is rated 4.0 out of 5 by 8.
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