Filamin 2 CRISPR/Cas9 KO Plasmid (h): sc-402358
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Filamin 2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Filamin 2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Filamin 2 CRISPR/Cas9 KO Plasmid (h) | sc-402358 | 20 µg | $397.00 |
Overview
FLNC encodes filamin 2, a large actin-binding scaffold that crosslinks F-actin and couples the cytoskeleton to membrane receptors and signaling complexes. Filamin 2 coordinates mechanotransduction, cell shape, and adhesion dynamics by organizing actin networks and interacting with integrins and associated focal adhesion machinery. In striated muscle, FLNC supports sarcomeric and myofibrillar integrity and contributes to force transmission and stress responses. Genetic perturbation of FLNC is associated with cardiomyopathies and myofibrillar myopathies, making it a relevant target for studying cytoskeletal remodeling and muscle disease mechanisms.
Filamin 2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the FLNC gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the FLNC together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the FLNC open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Filamin 2 protein expression.
This CRISPR knockout system enables efficient generation of FLNC-deficient cell models for investigation of Filamin 2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting FLNC exon(s) critical for Filamin 2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple FLNC genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Filamin 2 CRISPR/Cas9 KO Plasmid (h) and Filamin 2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the FLNC locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Filamin 2 HDR Plasmid (h) and Filamin 2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by FLNC homology arms to support homology-directed repair at defined FLNC target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.