Date published: 2025-9-5
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Fibronectin Antibody (EP5) - Immunohistochemistry - Image 349132
纤连蛋白抗体 (EP5): sc-8422. 福尔马林固定, 石蜡包埋的人胎盘组织的免疫过氧化物酶染色显示细胞外基质染色.
纤连蛋白抗体 (EP5): sc-8422. 福尔马林固定, 石蜡包埋的人胎盘组织的免疫过氧化物酶染色显示细胞外基质染色.

Fibronectin 抗体 (EP5): sc-8422

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说明书
  • Fibronectin 抗体 EP5 是小鼠单克隆 IgG1 κ，Fibronectin抗体, 在328篇文献中引用，规格为200 µg/ml
  • 针对human的T细胞白血病活检
  • Fibronectin 抗体 (EP5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Fibronectin
  • 抗Fibronectin抗体（EP5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Fibronectin (EP5): sc-8422的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Fibronectin 抗体 (EP5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Fibronectin 抗体 (EP5) 组合成套装提供。 (请参阅下面的订购信息)。

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研究信息

関連項目

纤连蛋白抗体（EP5）是一种小鼠单克隆IgG1κ轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的纤连蛋白。抗纤维蛋白原抗体（EP5）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。纤维连接蛋白是一种重要的细胞外基质糖蛋白，在细胞粘附、运动和伤口愈合中发挥着重要作用，是组织修复和再生所必需的。这种高分子量异二聚体蛋白存在于大多数细胞表面、细胞外液和血浆中，纤维连接蛋白的存在对于维持组织的结构完整性至关重要。纤维连接蛋白的结构特征是具有多个结构域，可促进与各种细胞表面受体和其他细胞外基质成分的相互作用，这对于细胞信号传导和组织结构非常重要。纤维粘连蛋白的替代剪接和糖基化可产生不同的同工型，其中一些与特定组织或病理状况（如恶性肿瘤）有关。在杜普伊特伦氏病中，肌成纤维细胞表型的形成与糖基化纤维粘连蛋白和剪接变体的存在有关，这凸显了纤维粘连蛋白在正常生理和疾病过程中的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Fibronectin 抗体 (EP5) 参考文献:

  1. 纤连蛋白, 层粘连蛋白和四连蛋白在人类乳腺癌中的分布，特别关注细胞外基质。  |  Christensen, L. 1992. APMIS Suppl. 26: 1-39. PMID: 1576006
  2. 单克隆抗体鉴定纤维粘连蛋白中央细胞结合域在细胞粘附, 细胞迁移和基质组装过程中发挥作用所需的两个远端位点。  |  Nagai, T., et al. 1991. J Cell Biol. 114: 1295-305. PMID: 1716636
  3. 纤连蛋白与病毒发病机制  |  Keski-Oja, J., et al. 1987. Rev Infect Dis. 9 Suppl 4: S404-11. PMID: 3326136
  4. 纤连蛋白： 分子结构、纤维结构和机械化学信号。  |  Dalton, CJ. and Lemmon, CA. 2021. Cells. 10: PMID: 34572092
  5. 能区分人体细胞和血浆纤维粘连蛋白的单克隆抗体。  |  Keen, J., et al. 1984. Mol Biol Med. 2: 15-27. PMID: 6083431
  6. 纤维粘连蛋白的结构及其在细胞粘附中的作用。  |  Akiyama, SK., et al. 1981. J Supramol Struct Cell Biochem. 16: 345-8. PMID: 6273596
  7. 纤连蛋白  |  Ruoslahti, E. 1981. J Oral Pathol. 10: 3-13. PMID: 6782207
  8. 纤维粘连蛋白的分子和生物相互作用。  |  Ruoslahti, E., et al. 1982. J Invest Dermatol. 79 Suppl 1: 65s-68s. PMID: 7045246
  9. 结节性掌状纤维瘤病中纤维粘连蛋白剪接变体, 同胎盘糖基化纤维粘连蛋白和层粘连蛋白同工酶的差异表达。  |  Kosmehl, H., et al. 1995. Pathol Res Pract. 191: 1105-13. PMID: 8822112
  10. 可溶性和不可溶性纤维连接蛋白可促进体外肺泡上皮伤口愈合。  |  Garat, C., et al. 1996. Am J Physiol. 271: L844-53. PMID: 8944729
  11. 纤连蛋白-整合素相互作用。  |  Johansson, S., et al. 1997. Front Biosci. 2: d126-46. PMID: 9159220
  12. 纤维粘连蛋白信使 RNA 在纤维化人肝和肝细胞癌中的表达, 定位和替代剪接模式。  |  Matsui, S., et al. 1997. J Hepatol. 27: 843-53. PMID: 9382972

订购信息

产品名称产品编号规格价格数量收藏夹

Fibronectin 抗体 (EP5)

sc-8422
200 µg/ml
$316.00

Fibronectin (EP5): m-IgG Fc BP-HRP 套装

sc-528228
200 µg Ab; 10 µg BP
$354.00

Fibronectin (EP5): m-IgGκ BP-HRP 套装

sc-520554
200 µg Ab, 40 µg BP
$354.00

Fibronectin (EP5): m-IgG1 BP-HRP 套装

sc-542827
200 µg Ab; 20 µg BP
$354.00

Fibronectin 抗体 (EP5) AC

sc-8422 AC
500 µg/ml, 25% agarose
$416.00

Fibronectin 抗体 (EP5) HRP

sc-8422 HRP
200 µg/ml
$316.00

Fibronectin 抗体 (EP5) FITC

sc-8422 FITC
200 µg/ml
$330.00

Fibronectin 抗体 (EP5) PE

sc-8422 PE
200 µg/ml
$343.00

Fibronectin 抗体 (EP5) Alexa Fluor® 488

sc-8422 AF488
200 µg/ml
$357.00

Fibronectin 抗体 (EP5) Alexa Fluor® 546

sc-8422 AF546
200 µg/ml
$357.00

Fibronectin 抗体 (EP5) Alexa Fluor® 594

sc-8422 AF594
200 µg/ml
$357.00

Fibronectin 抗体 (EP5) Alexa Fluor® 647

sc-8422 AF647
200 µg/ml
$357.00

Fibronectin 抗体 (EP5) Alexa Fluor® 680

sc-8422 AF680
200 µg/ml
$357.00

Fibronectin 抗体 (EP5) Alexa Fluor® 790

sc-8422 AF790
200 µg/ml
$357.00

Could you reccomend a product for staining fibronectin in cell culture?

Asked by: Il777
Thank you for your question. You can find our recommended protocol under this link: https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining.
Answered by: Tech Support Europe
Date published: 2024-07-16

Hola. Necesito un anticuerpo anti fibronectina que no sea producido en conejo y sirva para muestras de raton. Uds tienen?

Asked by: Anonymous
Thank you for your question. You can find all our Fibronectin Antibodies by following this link: https://www.scbt.com/browse/fibronectin-Antibodies/_/N-1ler6yd
Answered by: Tech Support Europe
Date published: 2024-10-19

Could you share a protocol for visualising fibronectin development in cell culture using fibronectin immunostaining?

Asked by: Il777
Thank you for your question. Here is our immunofluorescence staining protocol : https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining
Answered by: BlakeJ
Date published: 2024-07-12

Can anyone share a working protocol for immunostaining for sc-8422 AF488 for mouse tissue?

Asked by: shihan
Thank you for your question. Please refer to our IF protocol for direct staining (using a directly conjugated primary antibody, without any secondary antibody for detection). https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2025-01-16

This antibody (EP-5) is suggested as a replacement for the polyclonal N-20 antibody. Where is the epitope for EP-5? Will it recognize the 70 kDa N-terminal fragment of human fibronectin?

Asked by: KlaraB
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined. We have not specifically evaluated this antibody for reactivity with the 70 kDa N-terminal fragment of human fibronectin, but based on the Western blot data on our website, we have not observed any 70 kDa fragment specifically in the samples we have tested.
Answered by: Technical Support
Date published: 2024-12-02

Dear Santa Cruz, I wanted to ask you if is possible to have a free sample of this antibody. I am working with myocardial slices from swine and I would like to see if this antibody can work with my samples. kind regards Davide Maselli

Asked by: DavideMaselli
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: SCBT Heidelberg Support
Date published: 2024-10-28

Dear Santa Cruz: Glad to know that your company has this Fn ab from the mouse. I wonder if is possible to have a free 10 ug sample of this antibody. If it works well, we surely will buy one. Best regards, Zheng

Asked by: Beer
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2024-08-13

Is it possible to use the Fibronectin Antibody (EP5) for immunohistochemistry applications if I am working with mice tissue samples?

Asked by: Trav11
Yes, this is absolutely possible. We recommend using our directly conjugated mouse monoclonal primary antibodies for instances like this. Currently, the Fibronectin Antibody (EP5): sc-8422 is available conjugated to HRP (sc-8422 HRP), phycoerythrin (sc-8422 PE), fluorescein (sc-8422 FITC), Alexa Fluor® 488 (sc-8422 AF488) or Alexa Fluor® 647 (sc-8422 AF647).
Answered by: Tech Service 8
Date published: 2024-09-27
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Rated 5 out of 5 by from Good productsThis is my first time purchasing antibodies for my research, and my friend strongly recommended that I use SANTA CRUZ products.
Date published: 2024-10-03
Rated 5 out of 5 by from Suitable for western blottingIt can detect a very good band in western blotting.
Date published: 2024-08-18
Rated 5 out of 5 by from Ottimo prottoHo comprato questo articolo in po di tempo fa e sono molto soddisfatta
Date published: 2024-06-12
Rated 5 out of 5 by from Excellent for WBIt is a good antibody in WB. It works in dilution 1:1000.
Date published: 2024-03-19
Rated 5 out of 5 by from Good antibody for ICCWorks for GBM tissue and cell line. At least for the ICC
Date published: 2022-07-22
Rated 3 out of 5 by from 検出できます。WBで使用しました。非特異的なバンドが検出されますが問題ないと思います。目的の分子は検出できます。バンドの大きさもデータシートと同じ位置です。
Date published: 2020-12-01
Rated 4 out of 5 by from よいサンプルでいただきましたが、免疫染色できれいに染まりました。ウエスタンでも確認したいと思います。ありがとうございました。
Date published: 2020-08-19
Rated 5 out of 5 by from Good for immunohistochemistryTested in immunohistochemistry on mouse paraffin kidney sections. Good signal.
Date published: 2020-08-10
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Fibronectin 抗体 (EP5) is rated 4.3 out of 5 by 49.
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