Date published: 2025-9-7
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FAK Antibody (D-1) - Immunohistochemistry - Image 352997
FAK 抗体 (D-1): sc-271126. 福尔马林固定, 石蜡包埋的人上胃组织的免疫过氧化物酶染色, 显示腺细胞的胞浆染色.
FAK 抗体 (D-1): sc-271126. 福尔马林固定, 石蜡包埋的人上胃组织的免疫过氧化物酶染色, 显示腺细胞的胞浆染色.

FAK 抗体 (D-1): sc-271126

4.3(25)
寫評論提問

说明书
  • FAK 抗体 D-1 是小鼠单克隆 IgG1 κ，FAK抗体, 在50篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的FAK的N-terminus的氨基酸2-31之间
  • FAK 抗体 (D-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 FAK p125; 也和以下物种反应，包括： 和 equine, canine and porcine
  • 抗FAK抗体（D-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素偶联抗体，可用于WB、IHC(P)和ELISA实验
  • 关于如何获取FAK (D-1): sc-271126的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是FAK Antibody (D-1) for WB and IHC(P) applications. 的首选辅助检测试剂，这些试剂现与FAK Antibody (D-1) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

FAK抗体（D-1）是一种IgG1 κ小鼠单克隆FAK抗体（也称为PTK2抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类来源的FAK蛋白。FAK抗体（D-1）既可作为非共轭的抗FAK抗体形式提供，也可作为多种共轭形式的抗FAK抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。粘着斑激酶最初被鉴定为Src编码的pp60固有蛋白酪氨酸激酶活性的主要底物。FAK p125的推导氨基酸序列显示，它是一种细胞质蛋白酪氨酸激酶，其序列和结构组织与迄今为止描述的其他蛋白相比是独特的。通过免疫荧光定位p125表明，它主要存在于细胞粘着斑中，因此被命名为粘着斑激酶（FAK）。在修复烧伤伤口时，FAK仅集中在那些积极迁移和快速增殖的基底角质形成细胞的基底边缘，并被激活并定位到培养中扩散的角质形成细胞的粘着斑中。因此，据推测，FAK可能在人类伤口的再上皮化中具有重要的体内作用。已证明，在使用通过G蛋白偶联受体起作用的促有丝分裂神经肽或神经递质刺激细胞生长时，FAK蛋白酪氨酸激酶活性也会增加。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

FAK 抗体 (D-1)

sc-271126
200 µg/ml
$316.00

FAK (D-1): m-IgG Fc BP-HRP 套装

sc-527208
200 µg Ab; 10 µg BP
$354.00

FAK (D-1): m-IgG1 BP-HRP 套装

sc-532581
200 µg Ab; 20 µg BP
$354.00

FAK 抗体 (D-1) AC

sc-271126 AC
500 µg/ml, 25% agarose
$416.00

FAK 抗体 (D-1) HRP

sc-271126 HRP
200 µg/ml
$316.00

FAK 抗体 (D-1) FITC

sc-271126 FITC
200 µg/ml
$330.00

FAK 抗体 (D-1) PE

sc-271126 PE
200 µg/ml
$343.00

FAK 抗体 (D-1) Alexa Fluor® 488

sc-271126 AF488
200 µg/ml
$357.00

FAK 抗体 (D-1) Alexa Fluor® 546

sc-271126 AF546
200 µg/ml
$357.00

FAK 抗体 (D-1) Alexa Fluor® 594

sc-271126 AF594
200 µg/ml
$357.00

FAK 抗体 (D-1) Alexa Fluor® 647

sc-271126 AF647
200 µg/ml
$357.00

FAK 抗体 (D-1) Alexa Fluor® 680

sc-271126 AF680
200 µg/ml
$357.00

FAK 抗体 (D-1) Alexa Fluor® 790

sc-271126 AF790
200 µg/ml
$357.00

FAK 抗体 (D-1) B

sc-271126 B
200 µg/ml
$320.00

FAK (D-1) 中和勝肽

sc-271126 P
100 µg/0.5 ml
$68.00

Good afternoon, I wanted to inquire whether the FAK antibody (D-1): sc-271126 exclusively labels the non-phosphorylated FAK protein or if it labels total FAK protein?

Asked by: jenni
Thank you for contacting Santa Cruz Biotechnology. For assistance please contact technical service. please email scbt@scbt.com or call 800-457-3801.
Answered by: BlakeJ
Date published: 2025-01-16

What application is the blocking peptide sc-271126 P appropriate for?

Asked by: jenniferc
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-10-23
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Rated 4 out of 5 by from Works well for adipose samples in western blotI had not had success with other FAK antibodies in adipose tissue samples (almost no signal) and could only get P-FAK antibodies to work. This one worked well for my murine adipose samples, not strong, but good enough. I used nitrocellulose, 5% milk TBST block, and 1% milk TBST antibody solution.
Date published: 2021-03-01
Rated 4 out of 5 by from Works well in WBThis antibody works well in WB, I used it to detect FAK in Huh7 cells, dil: 1:500
Date published: 2020-02-21
Rated 4 out of 5 by from Good and specific signal in western blottingDilution 1:1000 Western blotting of GN11 neuronal cell lysate Good and specific signal with low background signal
Date published: 2019-12-13
Rated 5 out of 5 by from Good antibodyI bought this one and is much more sensitive than the earlier one
Date published: 2019-06-04
Rated 5 out of 5 by from 之前用的抗体都很好之前用过成骨的抗体,这次选择一些信号通路的抗体，但还在选择中，需要一些抗体试用，进行信号通路的筛选。
Date published: 2018-04-28
Rated 5 out of 5 by from Works great in WB!One clear band detected by WB, around 125 kDa, no background.
Date published: 2018-03-07
Rated 5 out of 5 by from great featuresI applied for the samples several days ago and they gave it to me immediately. very good and sensitive.
Date published: 2018-01-31
Rated 5 out of 5 by from Sehr gute Ergebnisse im Western Blot.Hab das Sample ausprobiert und sowohl CML Zellinien (rechts vom Marker) als auch MDAMB231 (links vom Marker) als Kontrollzellen (im Datenblatt angegeben) aufgetragen. Im Western Blot wird jeweils nur eine spezifische Bande bei 125 kDa detektiert. Passt
Date published: 2017-12-12
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FAK 抗体 (D-1) is rated 4.3 out of 5 by 25.
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