Date published: 2025-9-9

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Ezrin 抗体 (H-4): sc-398542

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说明书
  • Ezrin抗体(H-4)是小鼠单克隆IgG2a κ, 在1篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的Ezrin的C-terminus的氨基酸549-584之间
  • 抗-Ezrin 抗体 (H-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Ezrin, Ermin, Radixin and Moesin
  • 抗Ezrin抗体(H-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Ezrin (H-4): sc-398542免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRPm-IgGκ BP-HRP 是 Ezrin 抗体 (H-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Ezrin 抗体 (H-4) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Ezrin抗体(H-4)是一种IgG2aκ小鼠单克隆Ezrin抗体,可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源Ezrin、Ermin、Radixin和Moesin。Ezrin抗体(H-4)有非偶联抗Ezrin抗体形式,以及多种偶联形式的抗Ezrin抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Ezrin、Moesin和Radixin属于高同源肌动蛋白相关蛋白家族,仅位于质膜之下。这些蛋白质被认为参与介导细胞骨架和膜蛋白之间的相互作用。Ezrin是各种蛋白酪氨酸激酶的主要细胞质底物,包括表皮生长因子受体。Ezrin也被鉴定为cAMP依赖性蛋白激酶(A激酶)锚定蛋白,并被命名为AKAP78。Moesin和Radixin与Ezrin具有70%以上的同源性,并在各种细胞类型中共同表达。尽管同源性很高,但这三种蛋白质表现出独特的受体特异性磷酸化模式。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Ezrin 抗体 (H-4)

sc-398542
200 µg/ml
$316.00

Ezrin (H-4): m-IgG Fc BP-HRP 套装

sc-530837
200 µg Ab; 10 µg BP
$354.00

Ezrin (H-4): m-IgGκ BP-HRP 套装

sc-524383
200 µg Ab, 40 µg BP
$354.00

Ezrin (H-4): m-IgG2a BP-HRP 套装

sc-547607
200 µg Ab; 10 µg BP
$354.00

Ezrin 抗体 (H-4) AC

sc-398542 AC
500 µg/ml, 25% agarose
$416.00

Ezrin 抗体 (H-4) HRP

sc-398542 HRP
200 µg/ml
$316.00

Ezrin 抗体 (H-4) FITC

sc-398542 FITC
200 µg/ml
$330.00

Ezrin 抗体 (H-4) PE

sc-398542 PE
200 µg/ml
$343.00

Ezrin 抗体 (H-4) Alexa Fluor® 488

sc-398542 AF488
200 µg/ml
$357.00

Ezrin 抗体 (H-4) Alexa Fluor® 546

sc-398542 AF546
200 µg/ml
$357.00

Ezrin 抗体 (H-4) Alexa Fluor® 594

sc-398542 AF594
200 µg/ml
$357.00

Ezrin 抗体 (H-4) Alexa Fluor® 647

sc-398542 AF647
200 µg/ml
$357.00

Ezrin 抗体 (H-4) Alexa Fluor® 680

sc-398542 AF680
200 µg/ml
$357.00

Ezrin 抗体 (H-4) Alexa Fluor® 790

sc-398542 AF790
200 µg/ml
$357.00

Ezrin (H-4) 中和勝肽

sc-398542 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-398542 P appropriate for?

Asked by: AbPolly
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 3 out of 5 by from It seems to work in IF on HepG2 cellsI received a sample and tested it in IF at the following conditions: Cells: HepG2_20000 cell/well MW6 Blocking: BSA 5%_2h_RT Primary Ab: Ezrin (sc-398542)_1: 50 in BSA 1%_ ON+4 Secondary Ab: Anti mouse_FITC 1:1000 Hoechst: 1: 1000 ( 2 ug/ml) per 10' Comments: DAPI OK, Ezrin green signal is visible
Date published: 2021-09-29
Rated 5 out of 5 by from WBWe used Ezrin (H-4) antibody for WB in Mouse lung primary fibroblast, and it worked very well. We used anti-mouse IgG.
Date published: 2018-03-28
Rated 5 out of 5 by from Highly potent and very specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was very strong detection of the ~87 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested.
Date published: 2016-11-10
Rated 5 out of 5 by from Produced superb immunofluorescence membraneProduced superb immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-12
Rated 5 out of 5 by from Nice Western Blot data of Ezrin expression in ANice Western Blot data of Ezrin expression in A-431, Ramos, MCF7, HT-29 and K-562 whole cell lysates. -SCBT QC
Date published: 2013-12-16
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Ezrin 抗体 (H-4) is rated 4.6 out of 5 by 5.
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