Date published: 2026-10-22

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Ezrin 抗体 (3C12): sc-58758

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说明书
  • Ezrin 抗体 3C12 是小鼠单克隆 IgG1 κ,Ezrin抗体, 在71篇文献中引用,规格为200 µg/ml
  • 免疫human物种的Ezrin的氨基酸362-585
  • Ezrin 抗体 (3C12) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat, human 和bovine 来源的 Ezrin
  • 抗Ezrin抗体(3C12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Ezrin Antibody (3C12) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Ezrin Antibody (3C12) 打包提供(请参阅下面的订购信息)。
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Ezrin抗体(3C12)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学检测小鼠、大鼠、人和牛的Ezrin蛋白。抗Ezrin抗体(3C12)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。Ezrin在连接细胞骨架和细胞膜方面起着至关重要的作用,这对于维持细胞形状和促进细胞信号传导至关重要。Ezrin主要位于质膜下方,与其他细胞骨架和膜蛋白相互作用,从而影响细胞粘附、迁移和形态等过程。值得注意的是,Ezrin是多种蛋白酪氨酸激酶(包括表皮生长因子受体)的主要细胞质底物,并作为A-激酶锚定蛋白(AKAP78)发挥作用。Ezrin与Moesin和Radixin的相互作用具有70%以上的同源性,这凸显了该蛋白家族在细胞动力学中的重要性,因为尽管这些蛋白具有相似性,但它们表现出不同的受体特异性磷酸化模式。这种特异性对于调节各种信号通路至关重要,使Ezrin成为细胞对外部刺激反应的关键参与者。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ezrin 抗体 (3C12) 参考文献:

  1. 由 ezrin, radixin 和 moesin 组成的基因家族。其特异性定位在肌动蛋白丝/质膜结合部位。  |  Sato, N., et al. 1992. J Cell Sci. 103 (Pt 1): 131-43. PMID: 1429901
  2. Moesin:4.1-talin-ezrin 蛋白家族的成员。  |  Lankes, WT. and Furthmayr, H. 1991. Proc Natl Acad Sci U S A. 88: 8297-301. PMID: 1924289
  3. 蛋白-酪氨酸激酶底物 ezrin 的 cDNA 克隆和测序显示其与带 4.1 同源。  |  Gould, KL., et al. 1989. EMBO J. 8: 4133-42. PMID: 2591371
  4. 星形胶质细胞中Ezrin和Radixin的前景:激酶、功能和病理。  |  Derouiche, A. and Geiger, KD. 2019. Int J Mol Sci. 20: PMID: 31382374
  5. 膜张力和Ezrin在吞噬过程中的作用。  |  Roberts, RE., et al. 2020. Adv Exp Med Biol. 1246: 83-102. PMID: 32399827
  6. Ezrin在哮喘相关气道炎症和重塑中的作用。  |  Zhao, S., et al. 2022. Mediators Inflamm. 2022: 6255012. PMID: 36530558
  7. Ezrin、radixin和moesin对于巨噬细胞迁移和细胞皮质力学是不可或缺的  |  Verdys, P., et al. 2024. EMBO J. 43: 4822-4845. PMID: 39026000
  8. ERM家族成员是细胞表面糖蛋白CD44和基于肌动蛋白的细胞骨架之间的分子连接体。  |  Tsukita, S., et al. 1994. J Cell Biol. 126: 391-401. PMID: 7518464
  9. Ezrin 具有在质膜上自我结合的特性。  |  Andréoli, C., et al. 1994. J Cell Sci. 107 (Pt 9): 2509-21. PMID: 7844168
  10. Ezrin 含有细胞骨架和膜结合结构域,因此被认为具有膜-骨架连接体的作用。  |  Algrain, M., et al. 1993. J Cell Biol. 120: 129-39. PMID: 8416983
  11. 酪氨酸激酶底物的 ezrin 样家族:受体特异性酪氨酸磷酸化模式及与恶性转化的关系。  |  Fazioli, F., et al. 1993. Oncogene. 8: 1335-45. PMID: 8479753
  12. Ezrin 是一种环磷酸腺苷依赖性蛋白激酶锚定蛋白。  |  Dransfield, DT., et al. 1997. EMBO J. 16: 35-43. PMID: 9009265

订购信息

产品名称产品编号规格价格数量收藏夹

Ezrin 抗体 (3C12)

sc-58758
200 µg/ml
$322.00

Ezrin (3C12): m-IgG Fc BP-HRP 套装

sc-528629
200 µg Ab; 10 µg BP
$361.00

Ezrin (3C12): m-IgGκ BP-HRP 套装

sc-521059
200 µg Ab, 40 µg BP
$361.00

Ezrin (3C12): m-IgG1 BP-HRP 套装

sc-543039
200 µg Ab; 20 µg BP
$361.00

Ezrin 抗体 (3C12) AC

sc-58758 AC
500 µg/ml, 25% agarose
$424.00

Ezrin 抗体 (3C12) HRP

sc-58758 HRP
200 µg/ml
$322.00

Ezrin 抗体 (3C12) FITC

sc-58758 FITC
200 µg/ml
$336.00

Ezrin 抗体 (3C12) PE

sc-58758 PE
200 µg/ml
$349.00

Ezrin 抗体 (3C12) Alexa Fluor® 488

sc-58758 AF488
200 µg/ml
$364.00

Ezrin 抗体 (3C12) Alexa Fluor® 546

sc-58758 AF546
200 µg/ml
$364.00

Ezrin 抗体 (3C12) Alexa Fluor® 594

sc-58758 AF594
200 µg/ml
$364.00

Ezrin 抗体 (3C12) Alexa Fluor® 647

sc-58758 AF647
200 µg/ml
$364.00

Ezrin 抗体 (3C12) Alexa Fluor® 680

sc-58758 AF680
200 µg/ml
$364.00

Ezrin 抗体 (3C12) Alexa Fluor® 790

sc-58758 AF790
200 µg/ml
$364.00

Does this antibody works on mouse tissue? does it works better in paraffin or OCT sections for IF?

Asked by: Pia Gr
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

For SC58758 AC, I’d like to ask if it conjugate Ezrin antibody through protein A/G, or directly conjugate the antibody? Thanks!

Asked by: Anonymous
Thank you for your question. Our antibodies are covalently bound to the agarose beads via primary amines. The protein G/A/L is covalently linked to the agarose bead.
Answered by: Tech Service
Date published: 2019-08-23

What is the recommended fixation method for IF with Ezrin Antibody (3C12): sc-58758?

Asked by: jenni
We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support 8
Date published: 2017-01-06
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Rated 4 out of 5 by MAO123 from Very good in Western BlottingWetstern blotting Dilution 1:500 Very strong and specfic signal in HEK cells. No signal in CFBE cells (low endogenous expression?)
Date published: 2020-06-18
Rated 4 out of 5 by Esteban from Good staining of apical RPE cells of the mouse eyeUsing a normal staining protocol for IF staining of 10um eye slides from mouse, I got a nicely stained Retinal pigmented epithelium cells.
Date published: 2019-07-17
Rated 3 out of 5 by dbaeza from FUNCIONAEl anticuerpo funciona pero usándolo a grandes concentraciones.
Date published: 2019-06-28
Rated 5 out of 5 by XGEG07 from Highly specific antibody for westernblotWesternblot of cell lysates works very nice and very specific!
Date published: 2018-05-14
Rated 4 out of 5 by mrca from Overall good antibodyThis antibody works very well for western blot analysis of mouse cerebellar extracts. When probing mixed primary cerebellar cultures, there is varied staining. This appears to have a filament like staining in primary culture, which is likely non-specific background.
Date published: 2018-01-30
Rated 5 out of 5 by cousins from excellent ezrin pattern on the cell surfaceCells from a monolayer got stained. However, the cells that did get stained showed excellent ezrin pattern on the cell surface.
Date published: 2017-02-15
Rated 5 out of 5 by Ellenn866 from GoodProducts have a good auxiliary effect, worthy of attention
Date published: 2017-01-10
Rated 5 out of 5 by Bill28 from Pleased with resultsOutstanding results for all applications including WB, IF and IHC
Date published: 2017-01-03
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Ezrin 抗体 (3C12) is rated 4.7 out of 5 by 18.
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