Date published: 2025-9-8

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Ezrin 抗体 (3C12): sc-58758

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说明书
  • Ezrin 抗体 3C12 是小鼠单克隆 IgG1 κ,Ezrin抗体, 在71篇文献中引用,规格为200 µg/ml
  • 免疫human物种的Ezrin的氨基酸362-585
  • Ezrin 抗体 (3C12) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat, human 和bovine 来源的 Ezrin
  • 抗Ezrin抗体(3C12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Ezrin (3C12): sc-58758免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 Ezrin 抗体 (3C12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Ezrin 抗体 (3C12) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Ezrin抗体(3C12)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学检测小鼠、大鼠、人和牛的Ezrin蛋白。抗Ezrin抗体(3C12)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。Ezrin在连接细胞骨架和细胞膜方面起着至关重要的作用,这对于维持细胞形状和促进细胞信号传导至关重要。Ezrin主要位于质膜下方,与其他细胞骨架和膜蛋白相互作用,从而影响细胞粘附、迁移和形态等过程。值得注意的是,Ezrin是多种蛋白酪氨酸激酶(包括表皮生长因子受体)的主要细胞质底物,并作为A-激酶锚定蛋白(AKAP78)发挥作用。Ezrin与Moesin和Radixin的相互作用具有70%以上的同源性,这凸显了该蛋白家族在细胞动力学中的重要性,因为尽管这些蛋白具有相似性,但它们表现出不同的受体特异性磷酸化模式。这种特异性对于调节各种信号通路至关重要,使Ezrin成为细胞对外部刺激反应的关键参与者。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ezrin 抗体 (3C12) 参考文献:

  1. 由 ezrin, radixin 和 moesin 组成的基因家族。其特异性定位在肌动蛋白丝/质膜结合部位。  |  Sato, N., et al. 1992. J Cell Sci. 103 (Pt 1): 131-43. PMID: 1429901
  2. Moesin:4.1-talin-ezrin 蛋白家族的成员。  |  Lankes, WT. and Furthmayr, H. 1991. Proc Natl Acad Sci U S A. 88: 8297-301. PMID: 1924289
  3. 蛋白-酪氨酸激酶底物 ezrin 的 cDNA 克隆和测序显示其与带 4.1 同源。  |  Gould, KL., et al. 1989. EMBO J. 8: 4133-42. PMID: 2591371
  4. 星形胶质细胞中Ezrin和Radixin的前景:激酶、功能和病理。  |  Derouiche, A. and Geiger, KD. 2019. Int J Mol Sci. 20: PMID: 31382374
  5. 膜张力和Ezrin在吞噬过程中的作用。  |  Roberts, RE., et al. 2020. Adv Exp Med Biol. 1246: 83-102. PMID: 32399827
  6. Ezrin在哮喘相关气道炎症和重塑中的作用。  |  Zhao, S., et al. 2022. Mediators Inflamm. 2022: 6255012. PMID: 36530558
  7. Ezrin、radixin和moesin对于巨噬细胞迁移和细胞皮质力学是不可或缺的  |  Verdys, P., et al. 2024. EMBO J. 43: 4822-4845. PMID: 39026000
  8. ERM家族成员是细胞表面糖蛋白CD44和基于肌动蛋白的细胞骨架之间的分子连接体。  |  Tsukita, S., et al. 1994. J Cell Biol. 126: 391-401. PMID: 7518464
  9. Ezrin 具有在质膜上自我结合的特性。  |  Andréoli, C., et al. 1994. J Cell Sci. 107 (Pt 9): 2509-21. PMID: 7844168
  10. Ezrin 含有细胞骨架和膜结合结构域,因此被认为具有膜-骨架连接体的作用。  |  Algrain, M., et al. 1993. J Cell Biol. 120: 129-39. PMID: 8416983
  11. 酪氨酸激酶底物的 ezrin 样家族:受体特异性酪氨酸磷酸化模式及与恶性转化的关系。  |  Fazioli, F., et al. 1993. Oncogene. 8: 1335-45. PMID: 8479753
  12. Ezrin 是一种环磷酸腺苷依赖性蛋白激酶锚定蛋白。  |  Dransfield, DT., et al. 1997. EMBO J. 16: 35-43. PMID: 9009265

订购信息

产品名称产品编号规格价格数量收藏夹

Ezrin 抗体 (3C12)

sc-58758
200 µg/ml
$316.00

Ezrin (3C12): m-IgG Fc BP-HRP 套装

sc-528629
200 µg Ab; 10 µg BP
$354.00

Ezrin (3C12): m-IgGκ BP-HRP 套装

sc-521059
200 µg Ab, 40 µg BP
$354.00

Ezrin (3C12): m-IgG1 BP-HRP 套装

sc-543039
200 µg Ab; 20 µg BP
$354.00

Ezrin 抗体 (3C12) AC

sc-58758 AC
500 µg/ml, 25% agarose
$416.00

Ezrin 抗体 (3C12) HRP

sc-58758 HRP
200 µg/ml
$316.00

Ezrin 抗体 (3C12) FITC

sc-58758 FITC
200 µg/ml
$330.00

Ezrin 抗体 (3C12) PE

sc-58758 PE
200 µg/ml
$343.00

Ezrin 抗体 (3C12) Alexa Fluor® 488

sc-58758 AF488
200 µg/ml
$357.00

Ezrin 抗体 (3C12) Alexa Fluor® 546

sc-58758 AF546
200 µg/ml
$357.00

Ezrin 抗体 (3C12) Alexa Fluor® 594

sc-58758 AF594
200 µg/ml
$357.00

Ezrin 抗体 (3C12) Alexa Fluor® 647

sc-58758 AF647
200 µg/ml
$357.00

Ezrin 抗体 (3C12) Alexa Fluor® 680

sc-58758 AF680
200 µg/ml
$357.00

Ezrin 抗体 (3C12) Alexa Fluor® 790

sc-58758 AF790
200 µg/ml
$357.00

Does this antibody works on mouse tissue? does it works better in paraffin or OCT sections for IF?

Asked by: Pia Gr
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

For SC58758 AC, I’d like to ask if it conjugate Ezrin antibody through protein A/G, or directly conjugate the antibody? Thanks!

Asked by: Anonymous
Thank you for your question. Our antibodies are covalently bound to the agarose beads via primary amines. The protein G/A/L is covalently linked to the agarose bead.
Answered by: Tech Service
Date published: 2019-08-23

What is the recommended fixation method for IF with Ezrin Antibody (3C12): sc-58758?

Asked by: jenni
We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support 8
Date published: 2017-01-06
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Rated 4 out of 5 by from Very good in Western BlottingWetstern blotting Dilution 1:500 Very strong and specfic signal in HEK cells. No signal in CFBE cells (low endogenous expression?)
Date published: 2020-06-18
Rated 4 out of 5 by from Good staining of apical RPE cells of the mouse eyeUsing a normal staining protocol for IF staining of 10um eye slides from mouse, I got a nicely stained Retinal pigmented epithelium cells.
Date published: 2019-07-17
Rated 3 out of 5 by from FUNCIONAEl anticuerpo funciona pero usándolo a grandes concentraciones.
Date published: 2019-06-28
Rated 5 out of 5 by from Highly specific antibody for westernblotWesternblot of cell lysates works very nice and very specific!
Date published: 2018-05-14
Rated 4 out of 5 by from Overall good antibodyThis antibody works very well for western blot analysis of mouse cerebellar extracts. When probing mixed primary cerebellar cultures, there is varied staining. This appears to have a filament like staining in primary culture, which is likely non-specific background.
Date published: 2018-01-30
Rated 5 out of 5 by from excellent ezrin pattern on the cell surfaceCells from a monolayer got stained. However, the cells that did get stained showed excellent ezrin pattern on the cell surface.
Date published: 2017-02-15
Rated 5 out of 5 by from GoodProducts have a good auxiliary effect, worthy of attention
Date published: 2017-01-10
Rated 5 out of 5 by from Pleased with resultsOutstanding results for all applications including WB, IF and IHC
Date published: 2017-01-03
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Ezrin 抗体 (3C12) is rated 4.7 out of 5 by 18.
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