Espin Antibody (31): sc-136136
- Espin Antibody (31) is a mouse monoclonal IgG2a, cited in 1 publications, provided at 50 µg/0.5 ml
- raised against amino acids 458-580 of Espin of rat origin
- recommended for detection of Espin of mouse, rat and human origin by WB and IP
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Espin Antibody (31). This work is in progress.
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Espin Antibody (31) is a mouse monoclonal IgG2a antibody that detects Espin in mouse, rat, and human samples through western blotting (WB) and immunoprecipitation (IP) applications. Espin, also known as autosomal recessive deafness type 36 protein (DFNB36), is an 854 amino acid cytoplasmic protein characterized by nine ANK repeats and one WH2 domain, which plays a crucial role in binding actin monomers and facilitating the assembly of actin bundles. This function is vital for maintaining the organization and dynamics of actin filament-rich structures that are essential for sensory transduction in various sensory cells, particularly in the inner ear. Proper functioning of Espin is critical, as defects in this protein lead to non-syndromic sensorineural deafness autosomal recessive type 36 (DFNB36), a condition that results from damage to the neural receptors of the inner ear or the associated nerve pathways to the brain. In humans, Espin is expressed in two isoforms due to alternative splicing, while in rodents, four major isoforms ranging from approximately 110 to 25 kDa have been identified, along with potential additional splice variants. Additionally, Espin has been shown to interact with proteins such as IRSp53 and Profilin-2, highlighting Espin′s importance in cellular signaling and structural integrity within sensory cells.
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Espin Antibody (31) References:
- Espin contains an additional actin-binding site in its N terminus and is a major actin-bundling protein of the Sertoli cell-spermatid ectoplasmic specialization junctional plaque. | Chen, B., et al. 1999. Mol Biol Cell. 10: 4327-39. PMID: 10588661
- Espin cross-links cause the elongation of microvillus-type parallel actin bundles in vivo. | Loomis, PA., et al. 2003. J Cell Biol. 163: 1045-55. PMID: 14657236
- Espin gene (ESPN) mutations associated with autosomal dominant hearing loss cause defects in microvillar elongation or organisation. | Donaudy, F., et al. 2006. J Med Genet. 43: 157-61. PMID: 15930085
- Espin actin-cytoskeletal proteins are in rat type I spiral ganglion neurons and include splice-isoforms with a functional nuclear localization signal. | Sekerková, G., et al. 2008. J Comp Neurol. 509: 661-76. PMID: 18551532
- Whirlin interacts with espin and modulates its actin-regulatory function: an insight into the mechanism of Usher syndrome type II. | Wang, L., et al. 2012. Hum Mol Genet. 21: 692-710. PMID: 22048959
- Actin-binding protein, Espin: a novel metastatic regulator for melanoma. | Yanagishita, T., et al. 2014. Mol Cancer Res. 12: 440-6. PMID: 24337068
- Espin distribution as revealed by super-resolution microscopy of stereocilia. | Qi, J., et al. 2020. Am J Transl Res. 12: 130-141. PMID: 32051742
- Espin overexpression causes stereocilia defects and provides an anti-capping effect on actin polymerization. | Zheng, L., et al. 2022. Cytoskeleton (Hoboken). 79: 64-74. PMID: 35844198
- Studies of functional properties of espin 1: Its interaction to actin filaments. | Yang, JB., et al. 2022. Front Cell Dev Biol. 10: 1022096. PMID: 36407097
- The role of Espin in the stereocilia regeneration and protection in Atoh1-overexpressed cochlear epithelium. | Yang, X., et al. 2023. Cell Prolif. 56: e13483. PMID: 37084708
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Espin Antibody (31) | sc-136136 | 50 µg/0.5 ml | $322.00 |