ERGIC-53 Antibody (F-3): sc-398777

ERGIC-53 Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects ERGIC-53 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). ERGIC-53 (F-3) antibody is available in various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ERGIC-53 protein, also known as LMAN1, plays a crucial role in intracellular transport of glycoproteins, specifically those with high mannose-type glycans, from the endoplasmic reticulum to the ER-Golgi intermediate compartment. ERGIC-53 functions as a mannose-specific lectin, recognizing sugar residues on glycoproteins and glycolipids, thereby facilitating their sorting and recycling within the cell. Proper functioning of ERGIC-53 is vital for maintaining cellular homeostasis and ensuring efficient protein trafficking; ERGIC-53 deficiency can lead to severe consequences, such as a rare autosomal recessive bleeding disorder characterized by a combined deficiency of coagulation factors V and VIII. Using anti-ERGIC-53 antibody (F-3) in research can provide valuable insights into glycoprotein transport mechanisms and ERGIC-53 dysfunction implications in various diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ERGIC-53 Antibody (F-3) References:

1. Molecular analysis of the ERGIC-53 gene in 35 families with combined factor V-factor VIII deficiency.  |  Neerman-Arbez, M., et al. 1999. Blood. 93: 2253-60. PMID: 10090934
2. ERGIC-53 gene structure and mutation analysis in 19 combined factors V and VIII deficiency families.  |  Nichols, WC., et al. 1999. Blood. 93: 2261-6. PMID: 10090935
3. ERGL, a novel gene related to ERGIC-53 that is highly expressed in normal and neoplastic prostate and several other tissues.  |  Yerushalmi, N., et al. 2001. Gene. 265: 55-60. PMID: 11255007
4. Oligomerization and interacellular localization of the glycoprotein receptor ERGIC-53 is independent of disulfide bonds.  |  Neve, EP., et al. 2005. J Mol Biol. 354: 556-68. PMID: 16257008
5. Transient dimerization and interaction with ERGIC-53 occur in the fibroblast growth factor receptor 3 early secretory pathway.  |  Lievens, PM., et al. 2008. Int J Biochem Cell Biol. 40: 2649-59. PMID: 18577465
6. Protein interaction profiling of the p97 adaptor UBXD1 points to a role for the complex in modulating ERGIC-53 trafficking.  |  Haines, DS., et al. 2012. Mol Cell Proteomics. 11: M111.016444. PMID: 22337587
7. A dual role for COOH-terminal lysine residues in pre-Golgi retention and endocytosis of ERGIC-53.  |  Kappeler, F., et al. 1994. J Biol Chem. 269: 6279-81. PMID: 8119975
8. ERGIC-53, a membrane protein of the ER-Golgi intermediate compartment, carries an ER retention motif.  |  Schindler, R., et al. 1993. Eur J Cell Biol. 61: 1-9. PMID: 8223692
9. Mistargeting of the lectin ERGIC-53 to the endoplasmic reticulum of HeLa cells impairs the secretion of a lysosomal enzyme.  |  Vollenweider, F., et al. 1998. J Cell Biol. 142: 377-89. PMID: 9679138
10. The recycling pathway of protein ERGIC-53 and dynamics of the ER-Golgi intermediate compartment.  |  Klumperman, J., et al. 1998. J Cell Sci. 111 (Pt 22): 3411-25. PMID: 9788882

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ERGIC-53 Antibody (F-3) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-ERGIC-53 Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ERGIC-53 Antibody (F-3) Specifications

Alternate Product Name: LMAN1

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 67-91

Epitope Target: ERGIC-53

Epitope Species: human

Product Reactivity/Detection: ERGIC-53

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for ERGIC-53 Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred ERGIC-53 antibody is ERGIC-53 Antibody (C-6): sc-365158.