Date published: 2025-10-23

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ERGIC-53 抗体 (F-3): sc-398777

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说明书
  • ERGIC-53 抗体 F-3 是小鼠单克隆 IgG1 κ,ERGIC-53抗体, 在5篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的ERGIC-53的N-terminus附近的氨基酸67-91之间
  • ERGIC-53 抗体 (F-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 ERGIC-53
  • 抗ERGIC-53抗体(F-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是ERGIC-53 Antibody (F-3) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与ERGIC-53 Antibody (F-3) 打包提供(请参阅下面的订购信息)。

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関連項目

ERGIC-53抗体(F-3)是一种IgG1 κ小鼠单克隆ERGIC-53抗体(也称为ERGIC53抗体、Gp58抗体、内质网-高尔基体中间区室蛋白53抗体、ER-高尔基体中间区室53 KDa蛋白抗体、细胞内甘露糖特异性凝集素MR60抗体、蛋白ERGIC-53抗体、F5F8D抗体、FMFD1抗体、MCFD1抗体、MR60抗体、凝集素甘露糖结合1抗体或凝集素甘露糖结合1抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的ERGIC-53蛋白。ERGIC-53抗体(F-3)可提供多种偶联形式,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。凝集素甘露糖结合1,也称为囊泡膜整合蛋白(VIP36),以及凝集素甘露糖结合2,也称为ER-高尔基体中间区室(ERGIC-53),它们组成了一个膜结合、普遍存在的蛋白家族,这些蛋白参与新合成的糖蛋白从内质网(ER)到ER-高尔基体中间区室(ERGIC)的选择性转运。VIP36在早期分泌途径中充当细胞内凝集素。它参与携带高甘露糖型聚糖的糖蛋白的分选和转运。ERGIC-53是一种甘露糖特异性凝集素,可识别糖蛋白和糖脂的糖残基。它介导蛋白质和/或脂质的分选和再循环。ERGIC-53(也称为LMAN1)的无表达会导致罕见的常染色体隐性出血性疾病,这种疾病会同时导致凝血因子V和VIII的缺乏。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ERGIC-53 抗体 (F-3) 参考文献:

  1. 对35个患有V因子和VIII因子缺乏症的家庭的ERGIC-53基因进行分子分析。  |  Neerman-Arbez, M., et al. 1999. Blood. 93: 2253-60. PMID: 10090934
  2. 19个第五和第八因子缺乏症合并家庭的ERGIC-53基因结构和突变分析。  |  Nichols, WC., et al. 1999. Blood. 93: 2261-6. PMID: 10090935
  3. ERGL是与ERGIC-53相关的新基因,在正常和肿瘤前列腺以及其他一些组织中高度表达。  |  Yerushalmi, N., et al. 2001. Gene. 265: 55-60. PMID: 11255007
  4. 糖蛋白受体 ERGIC-53 的寡聚化和细胞间定域键与二硫键无关。  |  Neve, EP., et al. 2005. J Mol Biol. 354: 556-68. PMID: 16257008
  5. 在成纤维细胞生长因子受体3的早期分泌途径中,ERGIC-53会发生瞬态二聚化和相互作用。  |  Lievens, PM., et al. 2008. Int J Biochem Cell Biol. 40: 2649-59. PMID: 18577465
  6. p97适配器UBXD1的蛋白质相互作用分析表明该复合物在调节ERGIC-53运输方面发挥作用。  |  Haines, DS., et al. 2012. Mol Cell Proteomics. 11: M111.016444. PMID: 22337587
  7. COOH 端赖氨酸残基在 ERGIC-53 的前高尔基滞留和内吞过程中发挥双重作用。  |  Kappeler, F., et al. 1994. J Biol Chem. 269: 6279-81. PMID: 8119975
  8. ERGIC-53是ER-高尔基中间区的一种膜蛋白,带有ER保留基序。  |  Schindler, R., et al. 1993. Eur J Cell Biol. 61: 1-9. PMID: 8223692
  9. 凝集素ERGIC-53在HeLa细胞内靶向内质网的错误,会损害溶酶体酶的分泌。  |  Vollenweider, F., et al. 1998. J Cell Biol. 142: 377-89. PMID: 9679138
  10. 蛋白质ERGIC-53的回收途径和ER-高尔基中间区的动态。  |  Klumperman, J., et al. 1998. J Cell Sci. 111 (Pt 22): 3411-25. PMID: 9788882

订购信息

产品名称产品编号规格价格数量收藏夹

ERGIC-53 抗体 (F-3)

sc-398777
200 µg/ml
$316.00

ERGIC-53 (F-3): m-IgG Fc BP-HRP 套装

sc-530925
200 µg Ab; 10 µg BP
$354.00

ERGIC-53 (F-3): m-IgGκ BP-HRP 套装

sc-524480
200 µg Ab, 40 µg BP
$354.00

ERGIC-53 (F-3): m-IgG1 BP-HRP 套装

sc-544321
200 µg Ab; 20 µg BP
$354.00

ERGIC-53 抗体 (F-3) AC

sc-398777 AC
500 µg/ml, 25% agarose
$416.00

ERGIC-53 抗体 (F-3) HRP

sc-398777 HRP
200 µg/ml
$316.00

ERGIC-53 抗体 (F-3) FITC

sc-398777 FITC
200 µg/ml
$330.00

ERGIC-53 抗体 (F-3) PE

sc-398777 PE
200 µg/ml
$343.00

ERGIC-53 抗体 (F-3) Alexa Fluor® 488

sc-398777 AF488
200 µg/ml
$357.00

ERGIC-53 抗体 (F-3) Alexa Fluor® 546

sc-398777 AF546
200 µg/ml
$357.00

ERGIC-53 抗体 (F-3) Alexa Fluor® 594

sc-398777 AF594
200 µg/ml
$357.00

ERGIC-53 抗体 (F-3) Alexa Fluor® 647

sc-398777 AF647
200 µg/ml
$357.00

ERGIC-53 抗体 (F-3) Alexa Fluor® 680

sc-398777 AF680
200 µg/ml
$357.00

ERGIC-53 抗体 (F-3) Alexa Fluor® 790

sc-398777 AF790
200 µg/ml
$357.00

ERGIC-53 (F-3) 中和勝肽

sc-398777 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-398777 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Didn't work for my WBI used this AB for WesternBlots with MCF7 whole cell lysate : nothing detected with ECL, several unspecific bands with high sensitivity detection reagent.
Date published: 2020-07-31
Rated 5 out of 5 by from Works fine for IF on HeLa cellsI tested this antibody for immunostaining HeLa cells. Worked better for PFA fixed HeLa cells at 1:60 dilution. 1:75 dilution gave little dispersed and faint staining.
Date published: 2018-02-16
Rated 3 out of 5 by from Not for mewas not successful with HFF in IF. maybe better in WB
Date published: 2017-12-19
Rated 3 out of 5 by from fainttested at 1:200 (mouse primary motor neurons) and 1:400 (mouse N2A cell line) and both resulted in very faint signal. Difficult to determine wether it is specific and will be tested again. Cells were fixed with PFA.
Date published: 2017-10-05
Rated 5 out of 5 by from Specific and robust in WBThis ERGIC-53 antibody is really strong and reliable for Western blot. Great choice!
Date published: 2017-02-08
Rated 5 out of 5 by from Used in WB and IF in mouse sample and itUsed in WB and IF in mouse sample and it works fine.
Date published: 2015-09-16
Rated 5 out of 5 by from Good antibody for westernGood antibody for western, clean background and specific band in human ERGIC-53 transfected 293T, HeLa, Hep G2, A549 and Jurkat whole cell lysates. - SCBT QC
Date published: 2015-06-16
Rated 5 out of 5 by from Clean blot with single strong band at expectedClean blot with single strong band at expected size using JAR whole cell lysate. -SCBT QC
Date published: 2015-04-02
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ERGIC-53 抗体 (F-3) is rated 4.1 out of 5 by 9.
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