Date published: 2025-9-6
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ERCC1 Antibody (D-10) - Western Blotting - Image 354323
ERCC1 抗体 (D-10): sc-17809. NIH/3T3 (A), COLO 320DM (B), MCF7 (C), MDA-MB-231 (D) 和 AMJ2-C8 (E) 全细胞裂解液中 ERCC1 表达的 Western 印迹分析.
ERCC1 抗体 (D-10): sc-17809. NIH/3T3 (A), COLO 320DM (B), MCF7 (C), MDA-MB-231 (D) 和 AMJ2-C8 (E) 全细胞裂解液中 ERCC1 表达的 Western 印迹分析.

ERCC1 抗体 (D-10): sc-17809

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寫評論提問

说明书
  • ERCC1 抗体 D-10 是小鼠单克隆 IgG2b κ，ERCC1抗体, 在61篇文献中引用，规格为200 µg/ml
  • 与氨基酸 1-297 结合，代表全长 DNA 切除修复蛋白 1（ERCC1），源自 human
  • ERCC1 抗体 (D-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 ERCC1
  • 抗ERCC1抗体（D-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-17809 X, 200 µg/0.1 ml)
  • 关于如何获取ERCC1 (D-10): sc-17809的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 ERCC1 抗体 (D-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ERCC1 抗体 (D-10) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

ERCC1抗体（D-10）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA ）。抗ERCC1抗体（D-10）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。ERCC1在核苷酸切除修复途径中起着至关重要的作用，该途径通过修复紫外线辐射等环境因素导致的DNA损伤来维持基因组的稳定性。ERCC1与XPF形成异二聚体，XPF是一种结构特异性内切酶，在DNA修复过程中的切割步骤中至关重要。ERCC1-XPF复合物能够识别并切割受损的DNA，从而促进去除体积较大的DNA加合物，防止可能导致疾病的突变，例如色素性干皮症，这是一种对阳光极度敏感并易患皮肤癌的疾病。ERCC1与各种DNA修复蛋白的相互作用凸显了其在细胞防御机制中对抗遗传毒性应激的重要性，使ERCC1成为癌症生物学和治疗干预研究的重要目标。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ERCC1 抗体 (D-10) 参考文献:

  1. 人类 DNA 修复切除核酸酶的组装顺序。  |  Wakasugi, M. and Sancar, A. 1999. J Biol Chem. 274: 18759-68. PMID: 10373492
  2. 人类切除修复基因 ERCC-1 的分子特征：cDNA 克隆及与酵母 DNA 修复基因 RAD10 的氨基酸同源性。  |  van Duin, M., et al. 1986. Cell. 44: 913-23. PMID: 2420469
  3. ERCC1作为非小细胞肺癌中铂类药物疗效的预测因子。  |  Postel-Vinay, S. and Soria, JC. 2017. J Clin Oncol. 35: 384-386. PMID: 27893323
  4. ERCC1在膀胱癌中的临床前景。  |  Koutsoukos, K., et al. 2020. Int J Mol Sci. 21: PMID: 33266377
  5. ERCC1在早期和晚期宫颈癌中的前景：从实验到临床应用。  |  Du, P., et al. 2022. Front Immunol. 13: 1065379. PMID: 36713431
  6. 用纯化的蛋白质成分重组哺乳动物 DNA 核苷酸切除修复。  |  Aboussekhra, A., et al. 1995. Cell. 80: 859-68. PMID: 7697716
  7. XPA 基因突变会阻碍与 ERCC1 的结合，从而导致核苷酸切除修复功能缺陷。  |  Li, L., et al. 1995. Mol Cell Biol. 15: 1993-8. PMID: 7891694
  8. 体外共同纠正 ERCC1, ERCC4 和 F 组色素性角化病 DNA 修复缺陷。  |  Biggerstaff, M., et al. 1993. EMBO J. 12: 3685-92. PMID: 8253090
  9. 分离出纠正色素性红斑狼疮细胞七个互补组缺陷的蛋白质因子。  |  Tateishi, S., et al. 1995. J Biochem. 118: 819-24. PMID: 8576098
  10. XPG 和 XPF/ERCC1 内切酶在紫外线诱导成纤维细胞 PCNA 免疫染色中的作用  |  Miura, M., et al. 1996. Exp Cell Res. 226: 126-32. PMID: 8660947
  11. 由结构特异性 DNA 修复内切酶缺陷引起的 F 组色素沉着病。  |  Sijbers, AM., et al. 1996. Cell. 86: 811-22. PMID: 8797827
  12. 对紫外线敏感的中国仓鼠卵巢 (CHO) 细胞系中的 ERCC1 突变。  |  Hayashi, T., et al. 1998. Mutat Res. 407: 269-76. PMID: 9653453

订购信息

产品名称产品编号规格价格数量收藏夹

ERCC1 抗体 (D-10)

sc-17809
200 µg/ml
$316.00

ERCC1 (D-10): m-IgG Fc BP-HRP 套装

sc-528322
200 µg Ab; 10 µg BP
$354.00

ERCC1 (D-10): m-IgGκ BP-HRP 套装

sc-520667
200 µg Ab, 40 µg BP
$354.00

ERCC1 (D-10): m-IgG2b BP-HRP 套装

sc-550029
200 µg Ab; 10 µg BP
$354.00

ERCC1 抗体 (D-10) X

sc-17809 X
200 µg/0.1 ml
$316.00

ERCC1 抗体 (D-10) AC

sc-17809 AC
500 µg/ml, 25% agarose
$416.00

ERCC1 抗体 (D-10) HRP

sc-17809 HRP
200 µg/ml
$316.00

ERCC1 抗体 (D-10) FITC

sc-17809 FITC
200 µg/ml
$330.00

ERCC1 抗体 (D-10) PE

sc-17809 PE
200 µg/ml
$343.00

ERCC1 抗体 (D-10) Alexa Fluor® 488

sc-17809 AF488
200 µg/ml
$357.00

ERCC1 抗体 (D-10) Alexa Fluor® 546

sc-17809 AF546
200 µg/ml
$357.00

ERCC1 抗体 (D-10) Alexa Fluor® 594

sc-17809 AF594
200 µg/ml
$357.00

ERCC1 抗体 (D-10) Alexa Fluor® 647

sc-17809 AF647
200 µg/ml
$357.00

ERCC1 抗体 (D-10) Alexa Fluor® 680

sc-17809 AF680
200 µg/ml
$357.00

ERCC1 抗体 (D-10) Alexa Fluor® 790

sc-17809 AF790
200 µg/ml
$357.00

Can i get this ERCC1 Antibody (D-10): sc-17809 conjugated with Alexa Fluor® 647 ? if so, can i know how long, how many, and how much it would be?

Asked by: Minjung
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2024-01-25

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue? Or even double or triple staining on the same samples?

Asked by: AbPolly
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to agarose (sc-17809 AC), HRP (sc-17809 HRP), phycoerythrin (sc-17809 PE), fluorescein (sc-17809 FITC), Alexa Fluor® 488 (sc-17809 AF488), and Alexa Fluor® 647 (sc-17809 AF647).
Answered by: Technical Support 7
Date published: 2017-01-09
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Rated 5 out of 5 by from Good antibodyThe antibody ERCC1 was used in immunofluorescence at 1:100 dilution. The nuclear staining was clear in paraformaldehyde fixed cells.
Date published: 2019-10-20
Rated 5 out of 5 by from Excellent antibodyVery good antibody, used at 1:500 dilution, low background and strong bands. Recognizes both human and murine ERCC1.
Date published: 2018-08-01
Rated 5 out of 5 by from perfectI used this antiboty 1:1000 for WB in DU145、C42 cell ,the band is clear
Date published: 2017-09-23
Rated 5 out of 5 by from GreatThe antibody is pretty good for IF staining. The background is very low.
Date published: 2017-08-02
Rated 3 out of 5 by from okGood antibody for western blot, but with a lot of background
Date published: 2017-02-09
Rated 5 out of 5 by from Outstanding antibody !!Great data for multiple applications including western blotting, IHC and IF
Date published: 2017-01-12
Rated 5 out of 5 by from Versatile AntibodyThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-06
Rated 3 out of 5 by from genericI use it in experiment,and found that the antibody has multiple bands.
Date published: 2016-11-10
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ERCC1 抗体 (D-10) is rated 4.7 out of 5 by 14.
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