Date published: 2025-9-7
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EPLIN Antibody (20) - Western Blotting - Image 359394
EPLIN 抗体 (20): sc-136399. HeLa (A), COLO 320DM (B) 和 Hep G2 (C) 全细胞裂解液中 EPLIN 表达的 Western 印迹分析.
EPLIN 抗体 (20): sc-136399. HeLa (A), COLO 320DM (B) 和 Hep G2 (C) 全细胞裂解液中 EPLIN 表达的 Western 印迹分析.

EPLIN 抗体 (20): sc-136399

4.5(4)
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说明书
  • EPLIN 抗体 20 是小鼠单克隆 IgG1 κ，EPLIN抗体, 在13篇文献中引用，规格为200 µg/ml
  • 针对与human来源的EPLIN的α同源物对应的氨基酸1-141
  • 推荐用于 mouse, rat, human 和 canine 来源的EPLIN WB, IP, IF 和 IHC(P)检测
  • 还可偶联琼脂糖 用于IP; 偶联HRP 用于WB, IHC(P) 和ELISA
  • 关于如何获取EPLIN (20): sc-136399的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 EPLIN 抗体 (20) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与EPLIN 抗体 (20) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

EPLIN抗体（20）是一种IgG1 κ小鼠单克隆EPLIN抗体（也称为EPLIN抗体），可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学（IHC（P））检测小鼠、大鼠、人类和犬类的EPLIN蛋白。EPLIN抗体（20）既有非偶联形式的抗EPLIN抗体，也有多种偶联形式的抗EPLIN抗体，包括琼脂糖、HRP。上皮蛋白在肿瘤中丢失（EPLIN）是一种与细胞骨架相关的蛋白，其特征是存在一个位于中央的lin-11、isl-1和mec-3（LIM）结构域。它还包含至少两个肌动蛋白结合域，其中C端结构域的结合效果优于N端结构域。通过与肌动蛋白单体和细丝结合，EPLIN参与肌动蛋白细胞骨架的调节，增加肌动蛋白应力纤维的数量和大小，延迟细丝成核，减少分支细丝的形成和肌动蛋白细丝的捆绑。它还抑制膜褶皱和肌动蛋白细丝解聚。EPLIN在胎盘、肾脏、胰腺、前列腺、卵巢、脾脏和心脏中强烈表达，在肺、肝脏、大脑、骨骼肌、胸腺、睾丸和肠道中表达程度较低。它以两种异构体形式表达，即EPLIN-alpha和EPLIN-beta。EPLIN-alpha表达的下调可能有助于侵袭性肿瘤细胞的运动能力。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

EPLIN 抗体 (20) 参考文献:

  1. EPLIN，肿瘤中丢失的上皮细胞蛋白。  |  Maul, RS. and Chang, DD. 1999. Oncogene. 18: 7838-41. PMID: 10618726
  2. 人类 EPLIN（肿瘤中丢失的上皮细胞蛋白）基因的特征研究揭示了两种 EPLIN 异构体不同的启动子。  |  Chen, S., et al. 2000. Gene. 248: 69-76. PMID: 10806352
  3. 小鼠肿瘤上皮细胞蛋白丢失（EPLIN）的特征以及哺乳动物和斑马鱼 EPLIN 的比较。  |  Maul, RS., et al. 2001. Gene. 262: 155-60. PMID: 11179679
  4. 瘤中上皮丢失蛋白（EPLIN）抑制转化的 NIH3T3 细胞的锚定依赖性生长需要 EPLIN 定位于肌动蛋白细胞骨架。  |  Song, Y., et al. 2002. Mol Biol Cell. 13: 1408-16. PMID: 11950948
  5. EPLIN 通过交联和稳定细丝来调节肌动蛋白的动态。  |  Maul, RS., et al. 2003. J Cell Biol. 160: 399-407. PMID: 12566430
  6. 类 NK 细胞细胞骨架的组成特征。  |  Meng, X. and Wilkins, JA. 2005. J Proteome Res. 4: 2081-7. PMID: 16335953
  7. EPLIN是RasV12转化细胞外排的关键调节因子。  |  Ohoka, A., et al. 2015. J Cell Sci. 128: 781-9. PMID: 25609711
  8. 胃癌中的EPLIN表达及其对预后和化疗耐药性的影响。  |  Gong, W., et al. 2021. Biomolecules. 11: PMID: 33917939
  9. 肿瘤中丢失的上皮蛋白EPLIN，癌症中的细胞和分子前景。  |  Zeng, J., et al. 2021. Biomolecules. 11: PMID: 34356662
  10. EPLIN是结直肠癌中的一种假定性肿瘤抑制因子，在耐药性中的意义  |  Zeng, J., et al. 2022. Int J Mol Sci. 23: PMID: 36499558
  11. EPLIN-β 是鸟氨酸脱羧酶抗原1的一种新型底物，可调节细胞迁移。  |  Li, D., et al. 2023. J Cell Sci. 136: PMID: 37325974
  12. SEPT9和EPLIN的共同作用可调节人类成纤维细胞的粘附和迁移。  |  Hecht, M., et al. 2024. Life Sci Alliance. 7: PMID: 38719752

订购信息

产品名称产品编号规格价格数量收藏夹

EPLIN 抗体 (20)

sc-136399
200 µg/ml
$316.00

EPLIN (20): m-IgG Fc BP-HRP 套装

sc-528821
200 µg Ab; 10 µg BP
$354.00

EPLIN (20): m-IgGκ BP-HRP 套装

sc-521365
200 µg Ab, 40 µg BP
$354.00

EPLIN (20): m-IgG1 BP-HRP 套装

sc-543152
200 µg Ab; 20 µg BP
$354.00

EPLIN 抗体 (20) AC

sc-136399 AC
500 µg/ml, 25% agarose
$416.00

EPLIN 抗体 (20) HRP

sc-136399 HRP
200 µg/ml
$316.00

Could you please send us a quote with estimated delivery time and shipping costs (to Tartu, Estonia) for: sc- 136399, EPLIN Antibody (20) ; sc- 136399. Qty: 200 ug Is it only possible to order 200 ug or are there other options available?

Asked by: Johanna
Thank you for contacting Santa Cruz Biotechnology. To request a price quote and ship date please email scbt@scbt.com
Answered by: BlakeJ
Date published: 2024-09-09

In which organism was the antigen produced, E.Coli or human cell lines?

Asked by: Kaarle
Thank you for your question. The antigen is produced from human.
Answered by: BlakeJ
Date published: 2024-03-13

Can EPLIN (20): sc-136399 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, EPLIN (20): sc-136399 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining.
Answered by: Technical Support 7
Date published: 2017-01-17
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Rated 4 out of 5 by from Good signal for WB and IFThe antibody was used for Western Blot and IF. The bands were visible
Date published: 2022-09-06
Rated 5 out of 5 by from Good antibody for WB and IHC(P)EPLIN (20) gave beautiful IHC(P) data in human stomach tissue.
Date published: 2017-01-14
Rated 5 out of 5 by from Great antibodyI obtain this antibody to use for Western blots few months ago. I have used Hek 293 cells and it has been working well so far.
Date published: 2016-12-14
Rated 4 out of 5 by from Produced great immunoperoxidase cytoskeletalProduced great immunoperoxidase cytoskeletal staining in formalin fixed, paraffin-embedded human lower stomach tissue. -SCBT QC
Date published: 2015-05-12
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EPLIN 抗体 (20) is rated 4.5 out of 5 by 4.
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