Date published: 2025-9-6

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EphB1 抗体 (5F10A4): sc-130054

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说明书
  • EphB1 抗体 5F10A4 是小鼠单克隆 IgG1 κ,EphB1抗体, 在2篇文献中引用,规格为200 µg/ml
  • 与重组蛋白结合,该重组蛋白与EphB1的19-133的氨基酸对应,来源于human
  • 推荐用于 mouse, rat 和 human 来源的EphB1 WB, IP, IF, IHC(P) 和 ELISA检测
  • 关于如何获取EphB1 (5F10A4): sc-130054免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 EphB1 抗体 (5F10A4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与EphB1 抗体 (5F10A4) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

EphB1抗体(5F10A4)是一种IgG1 κ小鼠单克隆EphB1抗体(也称为Eph受体B1抗体、NET抗体或ELK抗体),可通过WB、IP、IF、IHC(P)和ELISA检测来自小鼠、大鼠和人类的EphB1蛋白。EphB1抗体(5F10A4)可作为非偶联抗EphB1抗体使用。Eph亚家族代表了迄今为止发现的最大一组受体蛋白酪氨酸激酶。虽然这些受体的生物学活性尚待确定,但越来越多的证据表明它们参与了中枢神经系统功能和发育。人类来源的Eph亚家族受体(及其小鼠/鸟类同源物)包括EphA1(Eph)、EphA2(Eck)、EphA3(Hek4)、EphA4(Hek8)、EphA5(Hek7)、EphA6(Hek12)、EphA7(Hek11/MDK1)、EphA8(Hek3)、EphB1(Hek6)、EphB2(Hek5)、EphB3(Cek10,Hek2)、EphB4(Htk)、EphB5(Hek9)和EphB6(Mep)。Eph受体的配体包括ephrin-A4(LERK-4),它与EphA3和EphB1结合。此外,ephrin-A2(ELF-1)被描述为EphA4的配体,ephrin-A3(Ehk1-L)作为EphA5的配体,ephrin-B2(Htk-L)作为EphB4(Htk)的配体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

EphB1 抗体 (5F10A4) 参考文献:

  1. EphB1 在急性髓性白血病中的抑制作用:调节 DNA 损伤控制系统  |  Kampen, KR., et al. 2015. Mol Cancer Res. 13: 982-92. PMID: 25944917
  2. 恶性脑肿瘤中 EphB1 受体的悖论。  |  Wei, W., et al. 2017. Cancer Cell Int. 17: 21. PMID: 28194092
  3. 内皮细胞中 EphB1 与洞穴素-1 的相互作用可调节洞穴生物生成。  |  Tiruppathi, C., et al. 2020. Mol Biol Cell. 31: 1167-1182. PMID: 32238105
  4. EphB1能促进非小细胞肺癌树突状细胞的分化和成熟。  |  Xie, Y., et al. 2024. Cancer Lett. 582: 216567. PMID: 38070822
  5. EphB1 通过视网膜和视网膜 Müller 细胞的炎症途径导致视网膜损伤。  |  Liu, L., et al. 2024. Mol Vis. 30: 167-174. PMID: 38601015
  6. ELF-1的鉴定和克隆,ELF-1是Mek4和Sek受体酪氨酸激酶的发育表达配体。  |  Cheng, HJ. and Flanagan, JG. 1994. Cell. 79: 157-68. PMID: 7522971
  7. EPH相关受体蛋白酪氨酸激酶Htk配体的分子克隆。  |  Bennett, BD., et al. 1995. Proc Natl Acad Sci U S A. 92: 1866-70. PMID: 7534404
  8. 鉴定 Eph 受体酪氨酸激酶家族新成员 Ehk-3 的全长和截短形式。  |  Valenzuela, DM., et al. 1995. Oncogene. 10: 1573-80. PMID: 7731712
  9. 鉴定编码 MDK1(一种在小鼠神经系统中表达的新型受体酪氨酸激酶)变体的替代剪接 mRNA。  |  Ciossek, T., et al. 1995. Oncogene. 10: 97-108. PMID: 7824284
  10. 受体酪氨酸激酶 hek 和 elk 的配体:分离编码蛋白质家族的 cDNA。  |  Kozlosky, CJ., et al. 1995. Oncogene. 10: 299-306. PMID: 7838529
  11. 五种人类 EPH 样受体蛋白-酪氨酸激酶的 cDNA 克隆和组织分布。  |  Fox, GM., et al. 1995. Oncogene. 10: 897-905. PMID: 7898931
  12. eph相关酪氨酸激酶受体配体家族的分子特征。  |  Beckmann, MP., et al. 1994. EMBO J. 13: 3757-62. PMID: 8070404

订购信息

产品名称产品编号规格价格数量收藏夹

EphB1 抗体 (5F10A4)

sc-130054
200 µg/ml
$316.00

EphB1 (5F10A4): m-IgG Fc BP-HRP 套装

sc-528727
200 µg Ab; 10 µg BP
$354.00

EphB1 (5F10A4): m-IgGκ BP-HRP 套装

sc-521205
200 µg Ab, 40 µg BP
$354.00

EphB1 (5F10A4): m-IgG1 BP-HRP 套装

sc-543095
200 µg Ab; 20 µg BP
$354.00

Dear Santa Cruz I am wondering if you could address my two questions. 1) sc-9319 is described as being discontinued. This batch looks guaranteed for immunostaining so I am wondering whether you still have a stock to distribute or not.

Asked by: SetsuSahara
Thank you for your question. This goat polyclonal antibody, EphB1 (M-19): sc-9319, is now discontinued and no longer available for distribution. For a limited time, we are offering a free 10 µg sample of the suggested replacement for this antibody, EphB1 (5F10A4): sc-130054. Please contact our Technical Service Department if you would like to test the antibody before placing your order. Please call our European Support office toll-free at 00800 4573 8000, email europe@scbt.com, or contact us by live chat.
Answered by: Technical Support
Date published: 2017-10-02

I need to use an antibody to detect the protein in samples of avian origin. Do you know if sc-130054: EphB1 (5F10A4) mouse monoclonal antibody will work?

Asked by: DefinitelyNotMatt
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 1 out of 5 by from It didn't work for WBWe tried this antibody (1:200 dilution as suggested) with rat brain tissue. We couldn't see any band.
Date published: 2020-09-01
Rated 5 out of 5 by from works on cryosectionsI used a free sample of this antibody for IF on mouse brain sections. The signal is very clear, though an enhancement by TSA may be required.
Date published: 2017-10-26
Rated 5 out of 5 by from Positive detection of endogenous expressionPositive detection of endogenous expression of EphB1 by WB using MDA-MB-453, MCF-7 and T47-D whole cell lysates. -SCBT QC
Date published: 2013-03-02
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EphB1 抗体 (5F10A4) is rated 3.7 out of 5 by 3.
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