Date published: 2025-9-6

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EP4 抗体 (C-4): sc-55596

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说明书
  • EP4 抗体 C-4 是小鼠单克隆 IgG2b κ,EP4抗体, 在34篇文献中引用,规格为200 µg/ml
  • 免疫human物种的EP4的氨基酸329-488
  • EP4 抗体 (C-4) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 EP4
  • 抗EP4抗体(C-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取EP4 (C-4): sc-55596免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2b BP-HRP">m-IgG2b BP-HRPm-IgGκ BP-HRP是EP4 Antibody (C-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与EP4 Antibody (C-4) 打包提供(请参阅下面的订购信息)。

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関連項目

EP4抗体(C-4)是一种小鼠单克隆IgG2b kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类的EP4。EP4 (C-4) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。EP4是E-前列腺素受体家族的一员,在介导前列腺素E2的作用方面发挥着关键作用。前列腺素E2是一种重要的脂质介质,参与包括肾功能在内的多种生理过程。EP4主要在肾小球和肾集合管中表达,在那里EP4与Gs蛋白结合,刺激腺苷酸环化酶活性。这一作用会导致环磷酸腺苷(cAMP)水平升高,而cAMP对于调节肾小球张力并影响肾素释放至关重要。EP4的正常功能对于维持体液和电解质平衡至关重要,失调与多种肾脏疾病有关。了解肾脏生理学中的EP4有助于为高血压和慢性肾病等疾病提供潜在的治疗靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

EP4 抗体 (C-4) 参考文献:

  1. 前列腺素 E 受体与肾脏  |  Breyer, MD. and Breyer, RM. 2000. Am J Physiol Renal Physiol. 279: F12-23. PMID: 10894784
  2. 选择性激活 E 型前列腺素(3)受体可缩小心肌梗死面积。前列腺素对心脏保护作用的新见解。  |  Thiemermann, C. and Zacharowski, K. 2000. Pharmacol Ther. 87: 61-7. PMID: 10924742
  3. 前列腺素 E 受体 EP4 亚型在大鼠肾上腺肾小球上的表达:参与醛固酮的释放。  |  Muro, S., et al. 2000. Endocr J. 47: 429-36. PMID: 11075723
  4. 通过在人类子宫中表达的两种前列腺素 E 受体 EP3 亚型异构体的多种信号转导途径。  |  Kotani, M., et al. 2000. J Clin Endocrinol Metab. 85: 4315-22. PMID: 11095474
  5. G 蛋白偶联类固醇受体与肾脏。  |  Breyer, MD. and Breyer, RM. 2001. Annu Rev Physiol. 63: 579-605. PMID: 11181968
  6. 一种新型前列腺素 E 受体 4 (EP4) 小分子拮抗剂可诱导关节软骨再生。  |  Jin, Y., et al. 2022. Cell Discov. 8: 24. PMID: 35256606
  7. PGE2 可激活软骨下骨破骨细胞中的 EP4,从而调节骨关节炎。  |  Jiang, W., et al. 2022. Bone Res. 10: 27. PMID: 35260562
  8. 巨噬细胞通过 PGE2/EP4 通路唤起肝星状细胞自噬,促进非酒精性脂肪肝小鼠的肝纤维化。  |  Cao, Y., et al. 2022. Cell Mol Life Sci. 79: 303. PMID: 35588334
  9. PGE2-EP2/EP4信号通过驱动炎症性肿瘤微环境中的mregDC-Treg轴来引发免疫抑制。  |  Thumkeo, D., et al. 2022. Cell Rep. 39: 110914. PMID: 35675777
  10. EP2和EP4受体在恶性胶质瘤中的互补作用。  |  Qiu, J., et al. 2023. Br J Pharmacol. 180: 2623-2640. PMID: 37232020
  11. [前列腺素 E 受体的分子生物学研究--前列腺素 E 受体亚型和同工酶的多功能表达]。  |  Ichikawa, A. 1998. Nihon Rinsho. 56: 1813-8. PMID: 9702058
  12. 前列腺素 E 受体对肾功能的调节。  |  Breyer, MD., et al. 1998. Kidney Int Suppl. 67: S88-94. PMID: 9736261

订购信息

产品名称产品编号规格价格数量收藏夹

EP4 抗体 (C-4)

sc-55596
200 µg/ml
$316.00

EP4 (C-4): m-IgG Fc BP-HRP 套装

sc-528607
200 µg Ab; 10 µg BP
$354.00

EP4 (C-4): m-IgGκ BP-HRP 套装

sc-521027
200 µg Ab, 40 µg BP
$354.00

EP4 (C-4): m-IgG2b BP-HRP 套装

sc-550056
200 µg Ab; 10 µg BP
$354.00

EP4 抗体 (C-4) AC

sc-55596 AC
500 µg/ml, 25% agarose
$416.00

EP4 抗体 (C-4) HRP

sc-55596 HRP
200 µg/ml
$316.00

EP4 抗体 (C-4) FITC

sc-55596 FITC
200 µg/ml
$330.00

EP4 抗体 (C-4) PE

sc-55596 PE
200 µg/ml
$343.00

EP4 抗体 (C-4) Alexa Fluor® 488

sc-55596 AF488
200 µg/ml
$357.00

EP4 抗体 (C-4) Alexa Fluor® 546

sc-55596 AF546
200 µg/ml
$357.00

EP4 抗体 (C-4) Alexa Fluor® 594

sc-55596 AF594
200 µg/ml
$357.00

EP4 抗体 (C-4) Alexa Fluor® 647

sc-55596 AF647
200 µg/ml
$357.00

EP4 抗体 (C-4) Alexa Fluor® 680

sc-55596 AF680
200 µg/ml
$357.00

EP4 抗体 (C-4) Alexa Fluor® 790

sc-55596 AF790
200 µg/ml
$357.00

does this antibody block the binding of PGE2 to EP4?

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2023-11-07

Is there a blocking peptide available for this antibody?

Asked by: AbPolly
Thank you for your question. Unfortunately as this antibody is raised against a recombinant protein and not a short synthetic peptide it is not possible to design a blocking peptide for it.
Answered by: Technical Support 9
Date published: 2017-01-18
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Rated 5 out of 5 by from Works wellI used this antibody for both WB and immunofluorescence and I am satisfied of the results
Date published: 2018-11-27
Rated 5 out of 5 by from Worked well for WB applicationGave a nice band at 1:200 when probing mouse tissue lysates.
Date published: 2018-03-23
Rated 5 out of 5 by from Superb productVery nice western blot data; strong signal with low background; also great IHC staining data
Date published: 2017-02-01
Rated 5 out of 5 by from WBRight band size was detected in human derived samples, and almost there was no background.
Date published: 2017-01-17
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of EP4 expression in MOLT-4, CCRF-CEM, RBL-1 and C6 whole cell lysates.
Date published: 2017-01-10
Rated 5 out of 5 by from Works wellI used this antibody for both WB and immunofluorescence and I am satisfied of the results
Date published: 2016-12-13
Rated 4 out of 5 by from Publishable data in WB using rat brain &Publishable data in WB using rat brain & kidney lysate. -SCBT Publication Review
Date published: 2015-06-23
Rated 4 out of 5 by from Publishable data in WB using human HOGsPublishable data in WB using human HOGs -SCBT Publication Review
Date published: 2015-06-06
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EP4 抗体 (C-4) is rated 4.5 out of 5 by 13.
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