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Ep-CAM 抗体 (VU-1D9): sc-51681

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说明书
  • Ep-CAM抗体(VU-1D9)是小鼠单克隆IgG1 κ, 在8篇文献中引用,规格为200 µg/ml
  • 针对human来源的小细胞肺癌细胞系HG9
  • 推荐用于 human 来源的Ep-CAM WB, IP, IF, IHC(P) 和 FCM检测
  • 还可偶联藻红蛋白 以及 FITC 用于IF, IHC(P) 和 FCM
  • 看 Ep-CAM (C-10): sc-25308,可查看有标记的 Ep-CAM 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Ep-CAM Antibody (VU-1D9) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Ep-CAM Antibody (VU-1D9) 打包提供(请参阅下面的订购信息)。
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関連項目

上皮细胞粘附分子Ep-CAM,也称为肿瘤相关钙信号转导子1和MK-1,是一种单体膜糖蛋白,在大多数正常人类上皮和癌细胞中表达。人类Ep-CAM基因编码一种314个氨基酸的蛋白质,以两种形式表达,一种主要形式和一种次要形式,在用氨基糖苷类抑制剂衣霉素处理后减少。 Ep-CAM在多种癌症中过表达,因此是人类实体瘤可视化和治疗的潜在靶点。 Ep-CAM包含一个含有两个表皮生长因子样重复序列的细胞外结构域,随后是一个半胱氨酸贫乏区,这是分子粘附特性所必需的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ep-CAM 抗体 (VU-1D9) 参考文献:

  1. 上皮细胞粘附分子在正常和恶性结肠组织中的表达模式。  |  Xie, X., et al. 2005. World J Gastroenterol. 11: 344-7. PMID: 15637741
  2. 上皮细胞粘附分子(EpCAM)复合蛋白促进转录因子介导的多能性重编程。  |  Huang, HP., et al. 2011. J Biol Chem. 286: 33520-32. PMID: 21799003
  3. 上皮细胞粘附分子在低咽癌中过度表达,下调后抑制疾病的转移和扩散。  |  Mu, Y., et al. 2014. Oncol Lett. 8: 175-182. PMID: 24959240
  4. 上皮细胞粘附分子在部分肉瘤中表达,并与平滑肌肉瘤的细胞学非典型程度相关。  |  Ward, K., et al. 2015. Mol Clin Oncol. 3: 31-36. PMID: 25469266
  5. 上皮细胞粘附分子过度表达通过PTEN/AKT/mTOR途径调节鼻咽癌细胞的上皮-间质转化、干细胞性和转移。  |  Wang, MH., et al. 2018. Cell Death Dis. 9: 2. PMID: 29305578
  6. 肝细胞癌中上皮细胞粘附分子和甲胎蛋白的免疫组织化学共表达。  |  Lima, LDP., et al. 2018. Can J Gastroenterol Hepatol. 2018: 5970852. PMID: 30112355
  7. 膜相关上皮细胞粘附分子在其细胞内结构域被蛋白酶体有效降解之前,会被γ-分泌酶缓慢裂解。  |  Huang, Y., et al. 2019. J Biol Chem. 294: 3051-3064. PMID: 30598504
  8. 人类结肠腺癌细胞肿瘤球中上皮细胞粘附分子(EpCAM)的表达。  |  Gisina, AM., et al. 2020. Bull Exp Biol Med. 170: 135-141. PMID: 33231806
  9. 抗上皮细胞粘附分子RNA适配体结合脂质体阿霉素作为结肠癌小鼠肿瘤模型的有效靶向治疗。  |  Mashreghi, M., et al. 2021. Biotechnol Prog. 37: e3116. PMID: 33369269
  10. Ep-CAM:一种人类上皮抗原,是一种同源细胞粘附分子。  |  Litvinov, SV., et al. 1994. J Cell Biol. 125: 437-46. PMID: 8163559

订购信息

产品名称产品编号规格价格数量收藏夹

Ep-CAM 抗体 (VU-1D9)

sc-51681
200 µg/ml
$322.00

Ep-CAM (VU-1D9): m-IgG Fc BP-HRP 套装

sc-536850
200 µg Ab; 10 µg BP
$361.00

Ep-CAM (VU-1D9): m-IgGκ BP-HRP 套装

sc-534017
200 µg Ab; 40 µg BP
$361.00

Ep-CAM (VU-1D9): m-IgG1 BP-HRP 套装

sc-544857
200 µg Ab; 20 µg BP
$361.00

Ep-CAM 抗体 (VU-1D9) FITC

sc-51681 FITC
200 µg/ml
$336.00

Ep-CAM 抗体 (VU-1D9) PE

sc-51681 PE
200 µg/ml
$349.00

We are going to use this antibody first time with IHC -P application, which antigen retrivel can be used for this antibody (Acidic, citrate or alkaline, ph ? )please suggest Thank you

Asked by: Kaushal
Thank you for your question. You can find our recommended protocol with instructions on how to perform antigen unmasking under this link https://www.scbt.com/resources/protocols/immunoperoxidase-staining. We recommend using heat treatment with citrate buffer.
Answered by: Tech Support Europe
Date published: 2024-11-22

Can Ep-CAM (VU-1D9): sc-51681 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Ep-CAM (VU-1D9): sc-51681 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by LM from Worked in WB in MCF7 and AWorked in WB in MCF7 and A-431 whole cell lysates. -SCBT QC
Date published: 2015-04-22
Rated 5 out of 5 by LR from Robust signal in WB in human transfectedRobust signal in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-04-15
Rated 5 out of 5 by JM from Positive signal in WB in human transfectedPositive signal in WB in human transfected 293T and A-431 whole cell lysates. -SCBT QC
Date published: 2015-04-14
Rated 5 out of 5 by NZ from Good specificity in WB in MCF7 whole cell lysateGood specificity in WB in MCF7 whole cell lysate. -SCBT QC
Date published: 2015-04-10
Rated 5 out of 5 by SM from Beautiful immunoperoxidase membrane and cytoplasmicBeautiful immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human rectum tissue. -SCBT QC
Date published: 2015-02-13
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Ep-CAM 抗体 (VU-1D9) is rated 5.0 out of 5 by 5.
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