ENT1 Antibody (F-12): sc-377283

ENT1 Antibody (F-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects ENT1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). ENT1 (F-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The equilibrative nucleoside transporter 1 (ENT1), also known as solute carrier family 29 (nucleoside transporters), member 1, plays a crucial role in cellular uptake and release of nucleosides, which are essential for numerous physiological processes. Located primarily in the plasma membrane, ENT1 facilitates transport of hydrophilic nucleosides, such as adenosine, across cell membranes, thereby influencing critical functions like neurotransmission and platelet aggregation. This transport is vital for maintaining cellular homeostasis and regulating nucleoside availability to cell surface receptors, which can impact various signaling pathways. Additionally, ENT1 is significant in pharmacology, as ENT1 is involved in uptake of antiviral and anticancer nucleoside drugs, making ENT1 a target of interest in therapeutic research. With broad species reactivity and multiple applications, anti-ENT1 antibody (F-12) is an invaluable tool for researchers studying nucleoside transport mechanisms and their implications in health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ENT1 Antibody (F-12) References:

1. Localization of human equilibrative nucleoside transporters, hENT1 and hENT2, in renal epithelial cells.  |  Mangravite, LM., et al. 2003. Am J Physiol Renal Physiol. 284: F902-10. PMID: 12527552
2. Uridine recognition motifs of human equilibrative nucleoside transporters 1 and 2 produced in Saccharomyces cerevisiae.  |  Vickers, MF., et al. 2004. Nucleosides Nucleotides Nucleic Acids. 23: 361-73. PMID: 15043160
3. Subtype-specific regulation of equilibrative nucleoside transporters by protein kinase CK2.  |  Stolk, M., et al. 2005. Biochem J. 386: 281-9. PMID: 15500446
4. Residue 33 of human equilibrative nucleoside transporter 2 is a functionally important component of both the dipyridamole and nucleoside binding sites.  |  Visser, F., et al. 2005. Mol Pharmacol. 67: 1291-8. PMID: 15644498
5. Identification and mutational analysis of amino acid residues involved in dipyridamole interactions with human and Caenorhabditis elegans equilibrative nucleoside transporters.  |  Visser, F., et al. 2005. J Biol Chem. 280: 11025-34. PMID: 15649894

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ENT1 Antibody (F-12) has 26 citations in a variety of scientific publications.

Primary antibodies like Anti-ENT1 Antibody (F-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ENT1 Antibody (F-12) Specifications

Alternate Product Name: ENT1

Product Clone Name: F-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 226-340

Epitope Target: ENT1

Epitope Species: human

Product Reactivity/Detection: ENT1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 26 citations in various scientific publications

Available Conjugates/Forms for ENT1 Antibody (F-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin