Date published: 2025-9-8

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eIF5B 抗体 (D-9): sc-393564

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说明书
  • eIF5B 抗体 D-9 是小鼠单克隆 IgG1 κ,eIF5B抗体, 在6篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列721-1020位于human物种的eIF5B的C-terminus附近
  • eIF5B 抗体 (D-9) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 eIF5B; 也和以下物种反应,包括: 和 equine, canine and porcine
  • 抗eIF5B抗体(D-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取eIF5B (D-9): sc-393564免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是eIF5B Antibody (D-9) for WB applications. 的首选辅助检测试剂,这些试剂现与eIF5B Antibody (D-9) 打包提供(请参阅下面的订购信息)。

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関連項目

eIF5B抗体(D-9)是一种IgG1 κ型小鼠单克隆eIF5B抗体(也称为eIF5B抗体),它可以通过蛋白质印迹、免疫沉淀、免疫荧光和酶联免疫吸附试验检测小鼠、大鼠和人类来源的eIF5B蛋白。eIF5B抗体(D-9)既有非共轭形式的抗eIF5B抗体,也有多种共轭形式的抗eIF5B抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。在哺乳动物细胞中,翻译是通过起始因子在多肽链起始水平上进行控制的。真核翻译起始因子5(eIF5)催化与40S核糖体亚基结合的GTP的水解,这是随后40S和60S亚基结合形成80S起始复合物所必需的功能。eIF5B(真核翻译起始因子5B),也被称为翻译起始因子IF-2,是一种1120个氨基酸的细胞质蛋白,通过促进甲酰甲硫氨酸-tRNA与核糖体的结合,在一般翻译起始过程中发挥作用。eIF5B以钙和磷脂依赖的方式与抗凝蛋白膜联蛋白V相互作用。由于eIF5B在所有三个生命界中都是保守的,因此它也被称为通用起始因子。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

eIF5B 抗体 (D-9) 参考文献:

  1. 参与核糖体:翻译的通用 IF。  |  Roll-Mecak, A., et al. 2001. Trends Biochem Sci. 26: 705-9. PMID: 11738593
  2. 启动因子 eIF5B 催化真核生物翻译启动过程中第二个依赖 GTP 的步骤。  |  Lee, JH., et al. 2002. Proc Natl Acad Sci U S A. 99: 16689-94. PMID: 12471154
  3. 翻译起始复合体的结构洞察:通用起始复合体的幽灵。  |  Allen, GS. and Frank, J. 2007. Mol Microbiol. 63: 941-50. PMID: 17238926
  4. 通过定向羟基探针绘制真核生物引发剂 eIF5B 在 80S 核糖体上的位置图。  |  Unbehaun, A., et al. 2007. EMBO J. 26: 3109-23. PMID: 17568775
  5. 翻译起始因子 eIF5B 的分子遗传结构功能分析。  |  Shin, BS. and Dever, TE. 2007. Methods Enzymol. 429: 185-201. PMID: 17913624
  6. 白念珠菌和酿酒酵母翻译因子 eIF5B 的功能等同性。  |  Jun, KO., et al. 2008. Mol Cells. 25: 172-7. PMID: 18414002
  7. 肠道病毒 3C 蛋白酶裂解真核细胞启动因子 eIF5B。  |  de Breyne, S., et al. 2008. Virology. 378: 118-22. PMID: 18572216

订购信息

产品名称产品编号规格价格数量收藏夹

eIF5B 抗体 (D-9)

sc-393564
200 µg/ml
$316.00

eIF5B (D-9): m-IgG Fc BP-HRP 套装

sc-527694
200 µg Ab; 10 µg BP
$354.00

eIF5B (D-9): m-IgG1 BP-HRP 套装

sc-533067
200 µg Ab; 20 µg BP
$354.00

eIF5B 抗体 (D-9) AC

sc-393564 AC
500 µg/ml, 25% agarose
$416.00

eIF5B 抗体 (D-9) HRP

sc-393564 HRP
200 µg/ml
$316.00

eIF5B 抗体 (D-9) FITC

sc-393564 FITC
200 µg/ml
$330.00

eIF5B 抗体 (D-9) PE

sc-393564 PE
200 µg/ml
$343.00

eIF5B 抗体 (D-9) Alexa Fluor® 488

sc-393564 AF488
200 µg/ml
$357.00

eIF5B 抗体 (D-9) Alexa Fluor® 546

sc-393564 AF546
200 µg/ml
$357.00

eIF5B 抗体 (D-9) Alexa Fluor® 594

sc-393564 AF594
200 µg/ml
$357.00

eIF5B 抗体 (D-9) Alexa Fluor® 647

sc-393564 AF647
200 µg/ml
$357.00

eIF5B 抗体 (D-9) Alexa Fluor® 680

sc-393564 AF680
200 µg/ml
$357.00

eIF5B 抗体 (D-9) Alexa Fluor® 790

sc-393564 AF790
200 µg/ml
$357.00

What is the recommended fixation method for immunofluorescence staining with eIF5B (D-9): sc-393564 monoclonal antibody?

Asked by: Cweed
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from ExcellentI took excellent specific bands at the correct size
Date published: 2021-03-17
Rated 4 out of 5 by from Worked great in 293TUsed eIF5B at a 1:200 dilution for WB on NuPAGE gel using HEK293T WCE. Worked great. We did see a few background bands.
Date published: 2017-09-29
Rated 5 out of 5 by from Great antibodyAntibody works well - clear, specific band for western blot with 25 ug U87 cell lysates at 1:1000 primary antibody dilution.
Date published: 2017-09-10
Rated 5 out of 5 by from Good data seen in Western blot with NTERAGood data seen in Western blot with NTERA-2 cl.D1, HeLa, Jurkat and A549 whole cell lysates -SCBT QC
Date published: 2015-04-30
Rated 5 out of 5 by from Wonderful IF staining of the cytoplasm of methanolWonderful IF staining of the cytoplasm of methanol-fixed HeLa cells -SCBT QC
Date published: 2013-08-20
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eIF5B 抗体 (D-9) is rated 4.8 out of 5 by 5.
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