Date published: 2025-9-10
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eIF2Bδ Antibody (H-4) - Western Blotting - Image 136984
eIF2Bδ (H-4): sc-271795. K-562 (A), KNRK (B) 和 ES-2 (C) 全细胞裂解液中 eIF2Bδ 表达的 Western 印迹分析.
eIF2Bδ (H-4): sc-271795. K-562 (A), KNRK (B) 和 ES-2 (C) 全细胞裂解液中 eIF2Bδ 表达的 Western 印迹分析.

eIF2Bδ 抗体 (H-4): sc-271795

4.7(3)
寫評論提問

说明书
  • eIF2Bδ抗体(H-4)是小鼠单克隆IgG1 κ, 在2篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列224-503位于human物种的eIF2Bδ的C-terminus附近
  • 推荐用于 mouse, rat 和 human 来源的eIF2Bδ WB, IP, IF 和 ELISA检测
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是eIF2Bδ Antibody (H-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与eIF2Bδ Antibody (H-4) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加eIF抗体，包括eIF1, eIF1AY, eIF2 α, eIF2B α, eIF2B β, eIF2B δ, eIF2B ε, eIF2 β, eIF2B γ, eIF2C, eIF2C2, eIF2C4, eIF2D, eIF3 p110, eIF3 α和eIF3 β

真核细胞中的蛋白质合成启动受到蛋白质起始因子与RNA分子之间相互作用的调控。真核起始因子复合物eIF2B是一个由五个亚基组成的复合体，包括eIF2Bα、eIF2Bβ、eIF2Bγ、eIF2Bδ和eIF2Bε。在eIF2/GTP与40S核糖体亚基相互作用后，eIF2B复合物会催化eIF2复合物上的GDP与GTP的交换。仅eIF2Bε亚基就具有鸟苷酸交换因子（GEF）的活性，但在所有五个eIF2B亚基都存在的情况下，该活性会更强。eIF2的磷酸化会抑制eIF2B的GEF活性，而这种抑制需要eIF2Bα亚基的参与。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

eIF2Bδ 抗体 (H-4)

sc-271795
200 µg/ml
$316.00

eIF2Bδ (H-4): m-IgG Fc BP-HRP 套装

sc-537694
200 µg Ab; 10 µg BP
$354.00

eIF2Bδ (H-4): m-IgGκ BP-HRP 套装

sc-535042
200 µg Ab; 40 µg BP
$354.00

eIF2Bδ (H-4): m-IgG1 BP-HRP 套装

sc-545312
200 µg Ab; 20 µg BP
$354.00

What is the recommended fixation method for immunofluorescence staining with eIF2Bδ (H-4): sc-271795 monoclonal antibody?

Asked by: Cweed
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Use it for Western Blot (1:500) detectingUse it for Western Blot (1:500) detecting total protein lysates (20 micro-gram) from mouse embryonic fibroblast cell line. Very specific (one band only) and reasonably strong. Size is accurate, slightly above 55 Kd marker. I am satisfied with this antibody.
Date published: 2015-10-19
Rated 5 out of 5 by from Cytoplasmic localization of the eIF2B proteinCytoplasmic localization of the eIF2B protein shown by immunofluorescence of methanol fixed HeLa cells. -SCBT QC
Date published: 2015-02-26
Rated 5 out of 5 by from Robust signal obtained by Western Blot using KRobust signal obtained by Western Blot using K-562, KNRK and ES-2 whole cell lysates. Clean background. -SCBT QC
Date published: 2013-02-28
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eIF2Bδ 抗体 (H-4) is rated 4.7 out of 5 by 3.
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