Date published: 2025-9-11

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Egr-3 抗体 (A-7): sc-390967

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说明书
  • Egr-3 抗体 A-7 是小鼠单克隆 IgG2b κ,Egr-3抗体, 在12篇文献中引用,规格为200 µg/ml
  • 免疫human物种的Egr-3的氨基酸1-180
  • Egr-3 抗体 (A-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Egr-3
  • 抗Egr-3抗体(A-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-390967 X, 200 µg/0.1 ml)
  • 关于如何获取Egr-3 (A-7): sc-390967免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP 是 Egr-3 抗体 (A-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Egr-3 抗体 (A-7) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Egr-3抗体(A-7)是一种IgG2b κ小鼠单克隆Egr-3抗体(也被指定为EGR3抗体),能通过WB, IP, IF, IHC(P) 和 ELISA检测小鼠、大鼠和人类来源的Egr-3蛋白。Egr-3抗体(A-7)可以以非偶联的抗Egr-3抗体形式提供,也可以以多种偶联形式的抗Egr-3抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Egr-1、Egr-2、Egr-3和Egr-4是属于Egr C2H2型锌指蛋白家族的核转录因子,含有三个C2H2型锌指。作为即早蛋白,Egr转录因子能被多种细胞外刺激迅速诱导。它们通过转录、翻译和翻译后(包括糖基化、磷酸化和氧化还原)机制以及蛋白质-蛋白质相互作用等多个层面的多种机制受到严格的差异控制。Egr-3参与肌梭的发育,并在T细胞激活后20分钟表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Egr-3 抗体 (A-7) 参考文献:

  1. IL-2 通过 SP1 和 NFAT 而非 Egr-2 和 Egr-3 激活 FasL 启动子。  |  Xiao, S., et al. 1999. Eur J Immunol. 29: 3456-65. PMID: 10556800
  2. 慢性乙醇对大鼠大脑中 Egr-1 和 Egr-3 DNA 结合活性和蛋白质表达具有区域选择性影响。  |  Depaz, IM., et al. 2000. Neurochem Int. 37: 473-82. PMID: 10871699
  3. 整合 G 蛋白偶联受体信号途径以激活转录因子(EGR-3)。  |  Tan, X., et al. 2003. Genomics Proteomics Bioinformatics. 1: 173-9. PMID: 15629029
  4. Egr-2 和 Egr-3 是 T 细胞活化的负调控因子。  |  Safford, M., et al. 2005. Nat Immunol. 6: 472-80. PMID: 15834410
  5. 早期生长应答蛋白 EGR-4 和 EGR-3 与免疫炎症介质 NF-kappaB p50 和 p65 相互作用。  |  Wieland, GD., et al. 2005. J Cell Sci. 118: 3203-12. PMID: 16014385
  6. 对依赖 Egr3 的活动诱导基因的鉴定揭示了与 DNA 损伤反应和精神分裂症有关的基因。  |  Marballi, KK., et al. 2022. Transl Psychiatry. 12: 320. PMID: 35941129
  7. egr3是心脏瓣膜形态发生所需的机械敏感性转录因子基因。  |  da Silva, AR., et al. 2024. Sci Adv. 10: eadl0633. PMID: 38748804
  8. EGR3 通过诱导许旺细胞样分化抑制肿瘤进展  |  Chen, CH., et al. 2024. Adv Sci (Weinh). 11: e2400066. PMID: 38973154
  9. 神经系统中的 Egr 转录因子。  |  Beckmann, AM. and Wilce, PA. 1997. Neurochem Int. 31: 477-510; discussion 517-6. PMID: 9307998
  10. 人类锌指蛋白 EGR-4 是一种自动调节转录抑制因子。  |  Zipfel, PF., et al. 1997. Biochim Biophys Acta. 1354: 134-44. PMID: 9396630
  11. 电休克刺激后海马颗粒细胞中 Egr-1 和 Egr-3 的顺序表达。  |  O'Donovan, KJ., et al. 1998. J Neurochem. 70: 1241-8. PMID: 9489747
  12. 环孢素 A 敏感转录因子 Egr-3 可调节 Fas 配体的表达。  |  Mittelstadt, PR. and Ashwell, JD. 1998. Mol Cell Biol. 18: 3744-51. PMID: 9632757

订购信息

产品名称产品编号规格价格数量收藏夹

Egr-3 抗体 (A-7)

sc-390967
200 µg/ml
$316.00

Egr-3 (A-7): m-IgG Fc BP-HRP 套装

sc-530319
200 µg Ab; 10 µg BP
$354.00

Egr-3 (A-7): m-IgGκ BP-HRP 套装

sc-523756
200 µg Ab, 40 µg BP
$354.00

Egr-3 抗体 (A-7) X

sc-390967 X
200 µg/0.1 ml
$316.00

Egr-3 抗体 (A-7) AC

sc-390967 AC
500 µg/ml, 25% agarose
$416.00

Egr-3 抗体 (A-7) HRP

sc-390967 HRP
200 µg/ml
$316.00

Egr-3 抗体 (A-7) FITC

sc-390967 FITC
200 µg/ml
$330.00

Egr-3 抗体 (A-7) PE

sc-390967 PE
200 µg/ml
$343.00

Egr-3 抗体 (A-7) Alexa Fluor® 488

sc-390967 AF488
200 µg/ml
$357.00

Egr-3 抗体 (A-7) Alexa Fluor® 546

sc-390967 AF546
200 µg/ml
$357.00

Egr-3 抗体 (A-7) Alexa Fluor® 594

sc-390967 AF594
200 µg/ml
$357.00

Egr-3 抗体 (A-7) Alexa Fluor® 647

sc-390967 AF647
200 µg/ml
$357.00

Egr-3 抗体 (A-7) Alexa Fluor® 680

sc-390967 AF680
200 µg/ml
$357.00

Egr-3 抗体 (A-7) Alexa Fluor® 790

sc-390967 AF790
200 µg/ml
$357.00

Egr-3 Antibody (A-7): sc-390967 Can we use this product for supershift on EMSA?

Asked by: Kaoru
Thank you for your question. sc-390967 Egr-3 (A-7) is recommended for WB, IP, PAR,and ELISA.
Answered by: BlakeJ
Date published: 2025-04-04

Representative pics for Egr3 A7 antibody on the web site show different molecular weight with Western blot. Which size is a correct one?? I am using this Ab and always found the band at 50-52kD but estimated Egr3 should be 43 in mouse with Western blot.

Asked by: cell specimen
Thank you for your question. The predicted molecular weight of Egr-3 is 43 kDa. However, we have observed a higher molecular weight signal around 50-52 kDa in rat brain tissue extract and RPMI2650 whole cell lysate. The lower molecular weight signal detected in FHs 173We whole cell lysate appears lower due to a discrepancy with the molecular weight markers used for this test. We are going to retest the antibody and provide any updated data on our website as soon as possible. In the meantime, please feel free to contact our Technical Service Department for support in troubleshooting the antibody.
Answered by: Technical Support
Date published: 2017-06-29

I am using Egr-3 (A-7): sc-390967 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 2 out of 5 by from 非特異的バンドが多かったHeLa細胞を様々な増殖因子,ストレス,サイトカインなどで刺激したサンプルでWesternBlotを行ったが,ノンスぺのバンドが多く,目的の分子量付近に明確なバンドは検出できなかmった。ただし,HeLaでRgr-3が発現しているか否かの知見はないので,抗体の良否は判定できない。
Date published: 2024-05-31
Rated 5 out of 5 by from works very wellThis antibody works very well for immunofluorescence staining in mouse endothelial cell line. Dilution 1:200. Fixation was done with 4% paraformaldehyde or methanol.
Date published: 2017-07-23
Rated 5 out of 5 by from Exceptional immunoperoxidase nuclear andExceptional immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human gall bladder tissue. -SCBT QC
Date published: 2015-04-25
Rated 5 out of 5 by from Great specificity in WB against FHs 173WeGreat specificity in WB against FHs 173We whole cell lysate. -SCBT QC
Date published: 2015-04-15
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Egr-3 抗体 (A-7) is rated 4.3 out of 5 by 4.
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