EF-1 δ Antibody (G-7): sc-393797

EF-1 δ Antibody (G-7) is a mouse monoclonal IgG3 antibody that detects EF-1 δ in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). EF-1 δ, also known as EEF1D, is a crucial 281 amino acid subunit of the elongation factor-1 complex, which is essential for the accurate delivery of aminoacyl-tRNAs to the ribosome, thereby facilitating the elongation of mRNA during protein synthesis. EF-1 δ functions as a guanine nucleotide exchange factor, promoting the exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP) on EF-1 α, which is vital for maintaining the efficiency of translation. Notably, EF-1 δ has been implicated in interactions with HIV-1 Tat, suggesting a role in the repression of host-cell mRNA transcription, which can have significant implications for viral pathogenesis. Furthermore, EF-1 δ overexpression has been linked to oesophageal carcinoma and may negatively influence the prognosis of medulloblastomas, highlighting its importance in the regulation of the cell cycle and transcriptional elongation. Multiple isoforms of EF-1 δ, resulting from alternative splicing events, add another layer of complexity to its functional roles in cellular processes. Anti-EF-1 δ antibody (G-7) is an invaluable tool for researchers investigating the intricate mechanisms of protein synthesis and the pathological implications of EF-1 δ dysregulation.

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EF-1 δ Antibody (G-7) References:

1. Kinectin anchors the translation elongation factor-1 delta to the endoplasmic reticulum.  |  Ong, LL., et al. 2003. J Biol Chem. 278: 32115-23. PMID: 12773547
2. Comparative proteomic analysis of all-trans-retinoic acid treatment reveals systematic posttranscriptional control mechanisms in acute promyelocytic leukemia.  |  Harris, MN., et al. 2004. Blood. 104: 1314-23. PMID: 15142884
3. Proteomic analysis of the soluble and the lysosomal+mitochondrial fractions from rat pancreas: Implications for cerulein-induced acute pancreatitis.  |  García-Hernández, V., et al. 2012. Biochim Biophys Acta. 1824: 1058-67. PMID: 22713802
4. cdc2 kinase sets a memory phosphorylation signal on elongation factor EF-1 delta during meiotic cell division, which perdures in early development.  |  Minella, O., et al. 1994. Cell Mol Biol (Noisy-le-grand). 40: 521-5. PMID: 8061568
5. Elongation factor 1 delta is enhanced following exposure to ionizing radiation.  |  Jung, M., et al. 1994. Cancer Res. 54: 2541-3. PMID: 8168075
6. Reconstitution in vitro of the valyl-tRNA synthetase-elongation factor (EF) 1 beta gamma delta complex. Essential roles of the NH2-terminal extension of valyl-tRNA synthetase and of the EF-1 delta subunit in complex formation.  |  Bec, G., et al. 1994. J Biol Chem. 269: 2086-92. PMID: 8294461
7. DNA binding activity of the mammalian translation elongation complex: recognition of chromium- and transplatin-damaged DNA.  |  Wang, JF., et al. 1997. Arch Toxicol. 71: 450-4. PMID: 9209691
8. Interaction of the second coding exon of Tat with human EF-1 delta delineates a mechanism for HIV-1-mediated shut-off of host mRNA translation.  |  Xiao, H., et al. 1998. Biochem Biophys Res Commun. 244: 384-9. PMID: 9514931
9. Phosphorylation of elongation factor-1 (EF-1) by cdc2 kinase.  |  Bellé, R., et al. 1995. Prog Cell Cycle Res. 1: 265-70. PMID: 9552369
10. Modulation of elongation factor-1 delta (EF-1 delta) expression by oncogenes in human epithelial cells.  |  Kolettas, E., et al. 1998. Anticancer Res. 18: 385-92. PMID: 9568107

The preferred EF-1 δ antibody is EF-1 δ Antibody (A-5): sc-393731.