Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
EDG-1/S1P1/S1PR1 CRISPR/Cas9 KO Plasmid (h): sc-400413
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EDG-1/S1P1/S1PR1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the EDG-1/S1P1/S1PR1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EDG-1/S1P1/S1PR1 Antibody (A-6): sc-48356
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EDG-1/S1P1/S1PR1 CRISPR/Cas9 KO Plasmid (h) | sc-400413 | 20 µg | $397.00 |
Overview
S1PR1 (EDG-1/S1P1) encodes a G protein–coupled receptor for sphingosine-1-phosphate (S1P) that transduces extracellular lipid signaling into intracellular programs controlling cell migration, survival, and barrier function. Through coupling to Gi and downstream effectors such as PI3K–AKT, MAPK/ERK, and Rho family GTPases, S1PR1 regulates cytoskeletal dynamics, adhesion, and endothelial permeability. It is a central node in S1P pathway biology that influences vascular development and immune cell trafficking, with dysregulation implicated in inflammation-associated vascular dysfunction and tumor microenvironment remodeling. As a membrane receptor with context-dependent signaling outputs, S1PR1 is widely studied in models of angiogenesis, leukocyte egress, and endothelial homeostasis.
EDG-1/S1P1/S1PR1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the S1PR1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the S1PR1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the S1PR1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish EDG-1/S1P1/S1PR1 protein expression.
This CRISPR knockout system enables efficient generation of S1PR1-deficient cell models for investigation of EDG-1/S1P1/S1PR1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting S1PR1 exon(s) critical for EDG-1/S1P1/S1PR1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple S1PR1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by EDG-1/S1P1/S1PR1 CRISPR/Cas9 KO Plasmid (h) and EDG-1/S1P1/S1PR1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the S1PR1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by EDG-1/S1P1/S1PR1 HDR Plasmid (h) and EDG-1/S1P1/S1PR1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by S1PR1 homology arms to support homology-directed repair at defined S1PR1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.