ECHS1 CRISPR/Cas9 KO Plasmid (h): sc-406053
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ECHS1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ECHS1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ECHS1 CRISPR/Cas9 KO Plasmid (h) | sc-406053 | 20 µg | $397.00 |
Overview
ECHS1 encodes short-chain enoyl-CoA hydratase 1, a mitochondrial matrix enzyme in the fatty acid β-oxidation spiral that catalyzes hydration of short-chain trans-2-enoyl-CoA intermediates. Through its role in energy metabolism, ECHS1 supports mitochondrial acetyl-CoA production and intersects with pathways that sustain redox balance and oxidative phosphorylation under nutrient stress. Perturbation of ECHS1 function disrupts mitochondrial substrate utilization and is associated with inborn errors of metabolism featuring lactic acidosis and neurodegenerative phenotypes. In cell models, ECHS1 is commonly studied for its impact on mitochondrial bioenergetics, metabolite flux, and stress-responsive signaling.
ECHS1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ECHS1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ECHS1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ECHS1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ECHS1 protein expression.
This CRISPR knockout system enables efficient generation of ECHS1-deficient cell models for investigation of ECHS1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ECHS1 exon(s) critical for ECHS1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ECHS1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ECHS1 CRISPR/Cas9 KO Plasmid (h) and ECHS1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ECHS1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ECHS1 HDR Plasmid (h) and ECHS1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ECHS1 homology arms to support homology-directed repair at defined ECHS1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.