EAAT1抗体 (A-3) 推荐用于 mouse, rat 和 human 来源的EAAT1 WB, IP, IF 和 ELISA检测

EAAT1 抗体 (A-3): sc-515839

EAAT1抗体 (A-3) is rated 4.4 out of 5 by 5.
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说明书

    • 抗-EAAT1 抗体 A-3 是小鼠单克隆 IgG2a κ,EAAT1抗体, 在10篇文献中引用,规格为200 µg/ml
    • 免疫human来源EAAT1近N-terminus的1-50氨基酸
    • 抗-EAAT1 抗体 (A-3) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 EAAT1
    • 抗-EAAT1 抗体 (A-3) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取EAAT1 (A-3): sc-515839免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for EAAT1 Antibody (A-3) for WB applications. This reagent is now offered in a bundle with EAAT1 Antibody (A-3) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

My EAAT1 antibody did not show up on phuman primary astrocytes in 1:400 or 1:1000 dilution? What would you recommend me?

Asked by: bdpower
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-12-11

I recently bought the Antibody goat anti human Glast/EAAT1 (A-3): sc-515839. I want to detect different taste bud cells and therefor one suitable marker is Glast. I do not have any signal.

Asked by: Dominik1987
Thank you for your inquiry and your interest in this product. We want you to be successful in your research. Please contact our Technical Service in our European Support Office in Germany directly. You can reach us by phone at: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com We are happy to assist you.
Answered by: Technical Support Europe
Date published: 2019-07-23
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Rated 4 out of 5 by from Buono in immuno e wb Ho utilizzato questo anticorpo sia in immunofluorescenza (diluizione usata 1:100), che in wb (usato 1:1000) su midollo spinale di topo e sono rimasta molto soddisfatta del risultato ottenuto
Date published: 2020-10-06
Rated 4 out of 5 by from Buon funzionamento Ho avuto questo prodotto come campione omaggio Lo ho utilizzato per western blot (1:500; 4°C, ON su estratti di NSC-derived astrocyte umani a diversi giorni di diffrenziamento. Funziona bene, un unica banda del peso molecolare atteso che aumenta di intensita con il grado di differenziamento
Date published: 2019-07-10
Rated 5 out of 5 by from Clean Western Blot- 1 band, correct size Tested this antibody at 1:500 dilution o/n on whole cell lysates (25µg protein/well). Clean western with only one bad at the right size.
Date published: 2018-04-03
Rated 4 out of 5 by from Good antibody I tried this antibody for IF. It worked very well on mouse brain.
Date published: 2017-09-25
Rated 5 out of 5 by from Strong signal Strong signal in the IPL of the retina of 4dpf zebrafish embryo. Sodium Citrate ph6 antigen retrieval was used,
Date published: 2017-02-11
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