E6-AP Antibody (E-5): sc-166532

E6-AP Antibody (E-5) is a mouse monoclonal IgG1 antibody that detects E6-AP in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). E6-AP, also known as ubiquitin protein ligase E3A (UBE3A), plays a crucial role in the ubiquitin-mediated proteolytic pathway, which maintains cellular homeostasis by selectively targeting proteins for degradation via the 26S proteasome. UBE3A antibody (E-5) recognizes a protein that conjugates ubiquitin to various substrates through enzymatic activity as an E3 ubiquitin ligase. E6-AP has been shown to interact with key proteins such as the tumor suppressor p53, forming a complex that enhances p53 ubiquitination, thereby regulating cellular levels. This interaction is particularly significant because accumulation of p53 due to impaired E6-AP activity can lead to severe consequences, including the development of Angelman syndrome, a neurodevelopmental disorder characterized by motor dysfunction and cognitive impairment. Anti-E6-AP antibody (E-5) serves as an invaluable tool for researchers studying protein degradation dynamics and E6-AP implications in various diseases, including cancer and genetic disorders.

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E6-AP Antibody (E-5) References:

1. E6-associated protein (E6-AP) is a dual function coactivator of steroid hormone receptors.  |  Ramamoorthy, S. and Nawaz, Z. 2008. Nucl Recept Signal. 6: e006. PMID: 18432313
2. UBE3A/E6-AP regulates cell proliferation by promoting proteasomal degradation of p27.  |  Mishra, A., et al. 2009. Neurobiol Dis. 36: 26-34. PMID: 19591933
3. E3 ubiquitin protein ligase, E6-associated protein (E6-AP) regulates PI3K-Akt signaling and prostate cell growth.  |  Srinivasan, S. and Nawaz, Z. 2011. Biochim Biophys Acta. 1809: 119-27. PMID: 20826237
4. Tristetraprolin and E6-AP: Killing the messenger in cervical cancer.  |  Sanduja, S. and Dixon, DA. 2010. Cell Cycle. 9: 3135-6. PMID: 20865819
5. E6-AP facilitates efficient transcription at estrogen responsive promoters through recruitment of chromatin modifiers.  |  Catoe, HW. and Nawaz, Z. 2011. Steroids. 76: 897-902. PMID: 21530567
6. Overexpression of ligase defective E6-associated protein, E6-AP, results in mammary tumorigenesis.  |  Ramamoorthy, S., et al. 2012. Breast Cancer Res Treat. 132: 97-108. PMID: 21553290
7. E6-AP association promotes SOD1 aggresomes degradation and suppresses toxicity.  |  Mishra, A., et al. 2013. Neurobiol Aging. 34: 1310.e11-23. PMID: 23040663
8. E6-AP/UBE3A protein acts as a ubiquitin ligase toward SOX9 protein.  |  Hattori, T., et al. 2013. J Biol Chem. 288: 35138-48. PMID: 24155239
9. LOC644656 promotes cisplatin resistance in cervical cancer by recruiting ZNF143 and activating the transcription of E6-AP.  |  Li, M., et al. 2024. Cell Signal. 117: 111115. PMID: 38395183
10. Ubiquitin, E6-AP, and their role in p53 inactivation.  |  Scheffner, M. 1998. Pharmacol Ther. 78: 129-39. PMID: 9690814

The preferred E6-AP antibody is E6-AP Antibody (E-4): sc-166689.