E2F-4 Antibody (WUF11): sc-56669

E2F-4 Antibody (WUF11) is a mouse monoclonal IgG1 antibody that detects E2F-4 in mouse, rat, and human samples through western blotting (WB) and immunoprecipitation (IP) applications. E2F-4 is a crucial member of the E2F family of transcription factors, which play a significant role in regulating the cell cycle, particularly during the transition from G1 phase to S phase. This regulation is vital for proper cell proliferation and differentiation, as dysregulation of E2F activity can lead to uncontrolled cell growth and is often implicated in various cancers. E2F-4 forms heterodimers with DP-1 protein, creating an active transcriptional regulatory complex that binds to specific DNA sequences to activate or repress target genes involved in cell cycle progression. E2F-4′s interaction with other proteins, including retinoblastoma (Rb) protein, further underscores E2F-4′s importance in cell cycle control, as Rb acts as a tumor suppressor by inhibiting E2F activity when unphosphorylated. The dynamic interplay between E2F-4 and Rb, along with other regulatory proteins, highlights the complexity of cell cycle regulation and the potential consequences of disruption, making E2F-4 a critical target for research in cancer biology and therapeutic interventions.

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E2F-4 Antibody (WUF11) References:

1. Expression of E2F-4 in invasive breast carcinomas is associated with poor prognosis.  |  Rakha, EA., et al. 2004. J Pathol. 203: 754-61. PMID: 15221934
2. E2F4 function in G2: maintaining G2-arrest to prevent mitotic entry with damaged DNA.  |  Plesca, D., et al. 2007. Cell Cycle. 6: 1147-52. PMID: 17507799
3. A screening of a library of T7 phage-displayed peptide identifies E2F-4 as an etoposide-binding protein.  |  Takami, M., et al. 2011. Molecules. 16: 4278-94. PMID: 21610657
4. Stable overexpression of p130/E2F4 affects the multipotential abilities of bone-marrow-derived mesenchymal stem cells.  |  Zhang, X., et al. 2018. J Cell Physiol. 233: 9739-9749. PMID: 29987913
5. Interplay between NRF1, E2F4 and MYC transcription factors regulating common target genes contributes to cancer development and progression.  |  Bhawe, K. and Roy, D. 2018. Cell Oncol (Dordr). 41: 465-484. PMID: 30047092
6. Activation of Wnt/β‑Catenin‑p130/E2F4 promotes the differentiation of bone marrow‑derived mesenchymal stem cells into type II alveolar epithelial cells through cell cycle arrest.  |  Zhang, X., et al. 2023. Exp Ther Med. 26: 330. PMID: 37346406
7. E2F-4 switches from p130 to p107 and pRB in response to cell cycle reentry.  |  Moberg, K., et al. 1996. Mol Cell Biol. 16: 1436-49. PMID: 8657117
8. E2F-1 but not E2F-4 can overcome p16-induced G1 cell-cycle arrest.  |  Mann, DJ. and Jones, NC. 1996. Curr Biol. 6: 474-83. PMID: 8723352
9. E2F4-RB and E2F4-p107 complexes suppress gene expression by transforming growth factor beta through E2F binding sites.  |  Li, JM., et al. 1997. Proc Natl Acad Sci U S A. 94: 4948-53. PMID: 9144170
10. The subcellular localization of E2F-4 is cell-cycle dependent.  |  Lindeman, GJ., et al. 1997. Proc Natl Acad Sci U S A. 94: 5095-100. PMID: 9144196

The preferred E2F-4 antibody is E2F-4 Antibody (RK-13): sc-511.