Date published: 2025-9-8
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Dvl-3/Dishevelled 3/DVL3 Antibody (4D3) - Western Blotting - Image 3419
Dvl-3 抗体 (4D3): sc-8027. MCF7 (A), ZR-75-1 (B) 和 BT-20 (C) 全细胞裂解液中 Dvl-3 表达的 Western 印迹分析.
Dvl-3 抗体 (4D3): sc-8027. MCF7 (A), ZR-75-1 (B) 和 BT-20 (C) 全细胞裂解液中 Dvl-3 表达的 Western 印迹分析.

Dvl-3/Dishevelled 3/DVL3 抗体 (4D3): sc-8027

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寫評論提問

说明书
  • Dvl-3/Dishevelled 3/DVL3抗体(4D3)是小鼠单克隆IgG1 κ, 在103篇文献中引用，规格为200 µg/ml
  • 免疫mouse来源Dvl-3位于C-terminus 的607-704氨基酸
  • 抗-Dvl-3/Dishevelled 3/DVL3 抗体 (4D3) 推荐用于 WB 和 IP，检测mouse, rat 和human 来源的 Dvl-3
  • 抗Dvl-3/Dishevelled 3/DVL3抗体（4D3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Dvl-3/Dishevelled 3/DVL3 (4D3): sc-8027的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是Dvl-3/Dishevelled 3/DVL3 Antibody (4D3) for WB applications. 的首选辅助检测试剂，这些试剂现与Dvl-3/Dishevelled 3/DVL3 Antibody (4D3) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Dishevelled抗体，包括Dvl, Dvl-1, Dvl-2和Dvl-3

Dvl-3抗体（4D3）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）和免疫沉淀（IP）检测小鼠、大鼠和人类样本中的Dvl-3。抗Dvl-3抗体（4D3）是针对小鼠来源的Dvl-3的氨基酸607-704制备的。Dvl-3 (4D3) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物，可满足不同实验需求。Dvl-3在Wnt信号传导通路中起着至关重要的作用，而Wnt信号传导通路对于细胞增殖、分化以及胚胎发育等细胞过程至关重要。Dvl-3主要位于细胞质中，参与将信号从毛状受体传导至下游效应器，从而影响发育和组织稳态过程中的细胞反应。抗Dvl-3抗体（4D3）使研究人员能够研究Wnt途径的机制，这一点尤为重要，因为该途径失调与包括癌症和神经退行性疾病在内的多种疾病有关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Dvl-3/Dishevelled 3/DVL3 抗体 (4D3) 参考文献:

  1. 果蝇节片极性基因 dishevelled 的小鼠同源物的分离和特征描述  |  Sussman, DJ., et al. 1994. Dev Biol. 166: 73-86. PMID: 7958461
  2. Dishevelled是果蝇褐飞虱信号通路的一个组成部分。  |  Krasnow, RE., et al. 1995. Development. 121: 4095-102. PMID: 8575310
  3. 在迪乔治综合征中，果蝇混乱片段-极性基因的人类同源序列被删除。  |  Pizzuti, A., et al. 1996. Am J Hum Genet. 58: 722-9. PMID: 8644734
  4. 果蝇散乱极性基因的两个人类同源物的 cDNA 表征和染色体图谱。  |  Pizzuti, A., et al. 1996. Hum Mol Genet. 5: 953-8. PMID: 8817329
  5. 小鼠 dishevelled-3 的分离和特征描述。  |  Tsang, M., et al. 1996. Dev Dyn. 207: 253-62. PMID: 8922524
  6. 一种frizzled同源物在脊椎动物Wnt信号通路中发挥作用  |  Yang-Snyder, J., et al. 1996. Curr Biol. 6: 1302-6. PMID: 8939578
  7. 人类鬅毛基因构成了一个含 DHR 的多基因家族。  |  Semënov, MV. and Snyder, M. 1997. Genomics. 42: 302-10. PMID: 9192851
  8. 在转染的哺乳动物细胞中，过表达小鼠 dishevelled-1 蛋白可抑制 GSK-3beta 介导的 tau 磷酸化。  |  Wagner, U., et al. 1997. FEBS Lett. 411: 369-72. PMID: 9271238

订购信息

产品名称产品编号规格价格数量收藏夹

Dvl-3/Dishevelled 3/DVL3 抗体 (4D3)

sc-8027
200 µg/ml
$316.00

Dvl-3/Dishevelled 3/DVL3 (4D3): m-IgG Fc BP-HRP 套装

sc-526510
200 µg Ab; 10 µg BP
$354.00

Dvl-3/Dishevelled 3/DVL3 (4D3): m-IgG1 BP-HRP 套装

sc-531883
200 µg Ab; 20 µg BP
$354.00

Dvl-3/Dishevelled 3/DVL3 抗体 (4D3) AC

sc-8027 AC
500 µg/ml, 25% agarose
$416.00

Dvl-3/Dishevelled 3/DVL3 抗体 (4D3) HRP

sc-8027 HRP
200 µg/ml
$316.00

Dvl-3/Dishevelled 3/DVL3 抗体 (4D3) FITC

sc-8027 FITC
200 µg/ml
$330.00

Dvl-3/Dishevelled 3/DVL3 抗体 (4D3) PE

sc-8027 PE
200 µg/ml
$343.00

Dvl-3/Dishevelled 3/DVL3 抗体 (4D3) Alexa Fluor® 488

sc-8027 AF488
200 µg/ml
$357.00

Dvl-3/Dishevelled 3/DVL3 抗体 (4D3) Alexa Fluor® 546

sc-8027 AF546
200 µg/ml
$357.00

Dvl-3/Dishevelled 3/DVL3 抗体 (4D3) Alexa Fluor® 594

sc-8027 AF594
200 µg/ml
$357.00

Dvl-3/Dishevelled 3/DVL3 抗体 (4D3) Alexa Fluor® 647

sc-8027 AF647
200 µg/ml
$357.00

Dvl-3/Dishevelled 3/DVL3 抗体 (4D3) Alexa Fluor® 680

sc-8027 AF680
200 µg/ml
$357.00

Dvl-3/Dishevelled 3/DVL3 抗体 (4D3) Alexa Fluor® 790

sc-8027 AF790
200 µg/ml
$357.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2017-01-09
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Rated 5 out of 5 by from Dvl-3 (4D3), sc-8027This antibody has worked perfectly for us in Western Blot on HUVEC lysates. It did not require any special optimization, but worked fine in 5% BSA at a suggested dilution (1:500). Upon stimulation with WNT3A ligand we detected an expected shift in bands.
Date published: 2018-12-13
Rated 5 out of 5 by from Excellent antibodyExcellent for WB (1:2500), IP (2ug) and IF (1:300). Specific for Dvl3 isoform in endothelial cells
Date published: 2018-03-09
Rated 5 out of 5 by from Superb westernGreat for western blot applications;strong, specific signal
Date published: 2017-01-27
Rated 5 out of 5 by from Reliable Antibody, Great for WBStrong, clear signal with little to no background. Great for WB. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-13
Rated 5 out of 5 by from CoIP using HEK293 cellsCoIP using HEK293 cells. -SCBT Publication Review
Date published: 2015-04-30
Rated 5 out of 5 by from Clean staining in paraffin embedded lungClean staining in paraffin embedded lung adenocarcinoma and squamous cell carcinoma sections using 1:50 dilution. -SCBT Publication Review
Date published: 2015-04-16
Rated 4 out of 5 by from Successful immunoprecipitation using 4D3Successful immunoprecipitation using 4D3 with Hek293T, Cos-7, and Cos-1 cells; verification via western blotting. -SCBT Publication Review
Date published: 2015-04-05
Rated 5 out of 5 by from Published western blot data in TCPublished western blot data in TC-32, 5838, and SKES whole cell lysates. -SCBT Publication Review
Date published: 2015-02-08
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Dvl-3/Dishevelled 3/DVL3 抗体 (4D3) is rated 4.9 out of 5 by 9.
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